Description
Book SynopsisIn situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes:
hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
hybridization prior to embedding (pre-embedding)
hybridization on ultrathin sections of frozen tissue (frozen tissue method).
For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.
The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is a
Table of ContentsGeneral Introduction. Abbreviations. Probes. Principles of Methodology. Sample Preparation. Post-Embedding Technique. Pre-Embedding Technique. Frozen Tissue Technique. Semithin Sections. Controls and Problems. Examples of Results. Appendices. Glossary. Index.
