Description
Book SynopsisThis second edition details new and updated chapters on key methodologies and breakthroughs in the mass spectrometry imaging (MSI) field. Chapters guide readers through nano-Desorption Electrospray Ionisation (nDESI), Matrix Assisted Laser Desorption Ionisation-2 (MALDI-2), Laser Ablation - Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) ,Imaging Mass Cytometry (IMC) with a variety of diverse samples including eye tissue, crop analysis, 3D cell culture models, and counterfeit goods analysis. Written in the format of the highly successfulMethods in Molecular Biologyseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge,Imaging Mass Spectrometry: Methods and Protocols, Second Edition aims to be auseful and practical guide to new researchersand experts looking to expand their knowledge.
Table of Contents1. MALDI and Trace Metal Analysis in Age Related Macular Degeneration
Joshua Millar, Susan Campbell, Catherine Duckett, Sarah Doyle, and Laura M. Cole
2. HistoSnap: a novel software tool to extract m/z-specific images from large MSHC datasets
K. Verheggen, N. Bhattacharya, M. Verhaert, B. Goossens, R. Sciot, and P. Verhaert
3. Spatially resolved quantitation of drug in skin equivalents using Mass Spectrometry Imaging (MSI)
Cristina Russo and Malcolm R. Clench
4. Update DESI Mass Spectrometry Imaging (MSI)
Emmanuelle Claude, Mark Towers, and Emrys Jones
5. Update Liquid Extraction Surface Analysis Mass Spectrometry Imaging of Denatured Intact Proteins
Emma K. Sisley, James W. Hughes, Oliver J. Hale, and Helen J. Cooper
6. MALDI MS imaging of cucumbers
Robert Bradshaw
7. The adaptation of the QV600 LLI Milli-Fluidics System to house ex vivo gastrointestinal tissue suitable for drug absorption and permeation studies, utilising MALDI-MSI and LC-MS/MS
Chloe E. Spencer, Catherine J Duckett, Stephen Rumbelow, and Malcolm R Clench
8. Ambient Mass Spectrometry Imaging by Water-Assisted Laser Desorption Ionization For Ex Vivo And In Vivo Applications
Nina Ogrinc, Paul Chaillou, Alexandre Kruszewski, Cristian Duriez, Michel Salzet, and Isabelle Fournier
9. Cytological cytospin preparation for the spatial proteomics analysis of thyroid nodules using MALDI-MSI
Isabella Piga, Fabio Pagni, Fulvio Magni, and Andrew Smith
10. Matrix effects free imaging of thin tissue sections using pneumatically assisted nano-DESI MSI
Leonidas Mavroudakis and Ingela Lanekoff
11. Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Materials
Joseph Ready and Callie Seaman
12. Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging
Maria K. Andersen, Marco Giampà, Elise Midtbust, Therese S. Høiem, Sebastian Krossa, and May-Britt Tessem
13. Multimodal Mass Spectrometry Imaging of an Aggregated 3D Cell Culture Model
Lucy Flint
14. Visualization of Small Intact Proteins in Breast Cancer FFPE tissue
Marco Giampà, Maria K. Andersen, Sebastian Krossa, Vanna Denti, Andrew Smith, and Siver Andreas Moestue
15. Negative Ion-mode N-glycan Mass Spectrometry Imaging by MALDI-2-TOF-MS
Jens Soltwisch and Bram Heijs
16. MS1-based data analysis approaches for FFPE tissue imaging of endogenous peptide ions by mass spectrometry histochemistry (MSHC)
Nivedita Bhattacharya, Konstantin Nagornov, Kenneth Verheggen, Marthe Verhaert, Raf Sciot, and Peter Verhaert
17. Mass Spectrometry Imaging: The Next Five Years
Malcolm R. Clench and Laura M. Cole
