Description
Book SynopsisFilling a gap in the literature for a hands-on guide focusing on everyday laboratory challenges, this English edition has been expanded and revised using the feedback received on the successful German precursor. Throughout the book, Professor Mascher draws on his 30 years of
experience and provides abundant practical advice, troubleshooting and other hints highlighted in boxes, as well as a broad selection of walkthrough case studies. Based on a course taught by the author, the first part of the book intuitively explains all steps of routine bioanalysis and explains how to set up a robust, inexpensive and effi cient method for a given substance. In the second part he includes 20 worked example cases that highlight common challenges and how to overcome them.
With its appendix containing tried-and-tested analytical methods for 100 clinically relevant substances from the author`s own laboratory,
complete with spectral and MS data as well as literature references and basic pharmacokinetic information, this is a life-long companion for
everyone working in clinical, pharmaceutical and biochemical analysis.
Comments to the German book:
"The book comes to life through its examples, showing not only what did work in the author's laboratory, but also what didn't."
ChemieReport
"Indispensable for novices, while even old hands will be able to expand their knowledge. A collection of analytical data for ca. 100 substances completes the book's offering, leaving almost nothing to be desired."
pharmind
Trade Review"The book is clearly written and comprehensive. A lot of practical advice and tips makes it helpful for any practically oriented clinical laboratory and it can be counted as a valuable source for everyone working with chromatographic techniques in a bioanalytical laboratory." (Analytical and Bioanalytical Chemistry, 13 November 2012)
“This book is more than a very successful and useful user guide, and is a valuable tool for the laboratorywork, not only for clinical analysts, but also for biochemists, pharmacists, etc. In addition to a compact targeted representation of the most important theoretical foundations for the planning and execution of (clinical) analysis (sample preparation, HPLC separation, usage of different modes, detection capabilities, derivatization techniques to the point of validation), the readers receive valuable information for their work, which are explained on the basis of practical examples [pages 1–64].” (ChemMedChem, 1 November 2012)
Table of ContentsPreface
INTRODUCTION
First Question: Determination of Ibuprofen in Plasma
Second Question: Determination of Tryptophan in Urine
Third Question: Determination of Paclitaxel in Tissue
PLANNING OF ANALYSES
Introduction
Limit of Detection (LOD) and Determination (LLOQ)
Detectors
Structure of the Analyte
Solubility of an Analyte
Selection of the Detector
SAMPLE PREPARATION
Dilution
Protein Precipitation, Overview
Extraction
HPLC SEPARATION
HPLC Pumps
Degassers
Injector
HPLC Columns
DETECTION
Detection in the Pharmaceutical/Bioanalytical Area
Detection in the "Clinical Area" (Therapy Control/Compliance)
CHEMICAL DERIVATISATION FOR DETECTION ENHANCEMENT
VALIDATION CONCEPTS
Introduction
Realisation of the FDA Guideline
PRACTICAL HINTS CONCERNING STABILITY, DESTRUCTION AND DEGRADATION PRODUCTS
METABOLITES
INTERNAL STANDARDS
CASE STUDIES WITH INTENSIVE DISCUSSION FOR EACH SUBSTANCE
Acetylcarnitine in Plasma
Acetylcysteine in Plasma
Acyclovir in Plasma and Urine
Caffeine in Plasma
Diazepam in Plasma
Diclofenac in Plasma
Dihydralazine in Plasma
Duramycin (Moli1901) in Plasma
Fluticasone Propionate in Plasma
Hydroxytriamterene Sulfate and Triamterene in Plasma and Urine
Ibuprofen in Plasma (also Enantiomeric Separation)
Minocycline in plasma
Norfloxacine in Plasma and Urine
Paclitaxel in Plasma, Urine, and Tissue
Paracetamol (Acetaminophen) in Plasma
Pimelic Acid in Plasma and Urine
8-Prenylnaringenin in Plasma and in Different Types of Tissues
Silibinin in Plasma
Valnemulin in Plasma, Different Tissue Types and Animal Feed
Vitamin B1 (Total Thiamine) in Plasma
APPENDIX
Short Description of Determination for about 100 Substances
Substances listed in the Appendix
A Short Explanation of Tables Presented in the Appendix
