Description
Book SynopsisThis volume provides readers with essential protocols for dissecting the host-fungus interaction, and engages researchers in study of mammalian disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Host-Fungal Interactions: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Table of Contents1. Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans
Carol A. Woolford and Aaron P. Mitchell
2. Serological Proteome Analysis for the Characterisation of Secreted Fungal Protein Antigens
Juliane Macheleidt and Olaf Kniemeyer
3. Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification
Quynh T. Phan and Scott G. Filler
4. CRISPR-Cas9 Mediated Gene Silencing in Cultured Human Epithelia Sara Gago, Nicola LD Overton, and Paul Bowyer
5. Analysis of Epithelial Cell Responses to Microbial Pathogens
Spyridoula Nikou, Jemima Ho, Olivia Hepworth, Nicole Ponde, Ruth Dickenson, Jonathan P. Richardson, and Julian R. Naglik
6. Single-cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluorescent Staining and Imaging Flow Cytometry
Margherita Bertuzzi and Gareth J. Howell
7.
8. Fungal Bioreporters to Monitor Outcomes of Aspergillus – Host Cell Interactions
Neta Shlezinger, Jeffrey Scott Fites, Bruce S. Klein, and Tobias M. Hohl
9. Candida albicans Interaction with Oral Epithelial Cells: Adhesion, Invasion, and Damage Assays
Selene Mogavero and Bernhard Hube
10. Experimental Evolution of Candida by Serial Passaging in Host Cells
Katja Graf, Bernhard Hube, and Sascha Brunke
11. Quantifying Receptor-mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells
Patawee Asamaphan, Gordon D. Brown, and Janet Willment
12. Measuring In Vivo Neutrophil Trafficking Responses during Fungal Infection Using Mixed Bone Marrow Chimeras
Rebecca A. Drummond and Michail S. Lionakis
13. Detection of Low Oxygen Microenvironments in a Murine Model of Invasive Pulmonary Aspergillosis Using Pimonidazole
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14. Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections
Jorge Amich
15. A Murine Model for Chronic A. fumigatus Airway Infections
Benjamin Ralph and Donald C. Sheppard
16. Automated Quantitative Analysis of Airway Epithelial Cell Detachment upon Fungal Challenge
Sayema Rahman, Darren D. Thomson, and Margherita Bertuzzi
