Description
Book SynopsisAnalytical techniques based on separation processes, such aschromatography and electrophoresis, are finding a growing range ofapplications in chemical, pharmaceutical and clinical laboratories.The Wiley Separation Science Series provides the analyst in theselaboratories with well focused books covering individualtechniques, so that they can be applied more efficiently andeffectively to contemporary analytical problems. In biotechnology,biochemistry and molecular biology, the characterization andanalysis of biomolecules such as proteins, oligosaccharides andnucleic acids are of great importance. High performance liquidchromatography (HPLC) has become one of the key analytical tools inthese fields, providing rapid purification and quantitativeanalysis of biomolecules.
High Performance Liquid Chromatography: Principles and Methods inBiotechnology covers the most important theoretical and practicalaspects of the application of HPLC to the biosciences, includingsample preparation,
Table of ContentsIntroduction to Chromatography in Biotechnology (C. Simpson).
The Principles of Separation by High Performance LiquidChromatography (R. Scott).
Column Selection in High Performance Liquid Chromatography (R.Eksteen).
Detection and Identification in Biochromatography (I. Krull, etal.).
Sample Preparation (A. Rizzi & I. Maurer-Fogy).
The Preparative HPLC of Biomolecules (G. Cox).
The Application of HPLC for Nucleic Acid Analysis (J. Wages &E. Katz).
High-pH Anion Exchange Chromatography of Recombinant GlycoproteinGlycans (R. Townsend).
The Application of HPLC for Proteins (K. Benedek).
Alternative Bioseparation Techniques (P. Righetti, et al.).
Index.
