Description
Book SynopsisOver the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms.
Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement.
Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Table of Contents
S.No
Tentative Chapter Title
Author Details
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Genome engineering as a tool for enhancing crop traits: Lessons from
CRISPR/Cas9
Tariq Shah and Aziz Khan*
College of Agriculture, Guangxi University, Nanning, China
Email: azizkhanturlandi@gmail.com
-
Commercialization of CRISPR derived Crops: Current Status and future prospects
Dr. Martin A Lema
Universidad Nacional de Quilmes, Roque Sáenz Peña 180, B1876BXD Bernal, Buenos Aires, Argentina. Email: mlema@unq.edu.ar
-
Targeted allele recombination for precise mapping in plant breeding
Dr. Eyal Fridman
Department of vegetables and field crops, Institute of Plant Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion 7505101, Israel
Email: fridmane@volcani.agri.gov.il
Hindex: 28
-
Applications of gene drive for weeds and pest management using CRISPR/CAS9 system in plants
Dr. Kathleen Hefferon
Food Science and Technology, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA
Email: klh22@cornell.edu Hindex: 14
-
Genome editing by Ribonucleorotein based delivery of CAS9 system in plants
Karina Morales, Michael J. Thomson
Professor and HM Beachell Endowed Chair in International Rice Improvement
Dept. of Soil and Crop Sciences, Texas A&M University / Texas A&M AgriLife Research
370 Olsen Blvd., 2474 TAMU, College Station, TX 77843-2474
Email: email: m.thomson@tamu.edu
Hindex: 28
-
Virus mediated delivery of CRISPR/CAS9 system in plants
Dr. Monika Bansal, Women scientist-A (completed)
School of Agricultural biotechnology,
Punjab Agricultural university, Ludhiana Dr. Shabir Hussain Wani
Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India
Email:shabirhwani@skuastkashmir.ac.in
Hindex: 24
Epigenetic and chromosomal Rearrangements in genome editing
Neha Goel (Ph.D.)
Genetics and Tree Improvement
Forest Research Institute, Dehradun Email:goelneha6@gmail.con
Dr. Shabir Hussain Wani
Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India
Email:shabirhwani@skuastkashmir.ac.in
Hindex: 24
-
Multiplexed genome editing in plants for improvement of various traits using CRISPR/CAS9 based system
Dr. Goetz Hensel
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Plant Reproductive Biology
Corrensstr. 3
D-06466 Seeland/OT Gatersleben
GERMANY
Email: hensel@ipk-gatersleben.de Hindex: 33
-
Application of CRISPR CAS9 for regulation of epigenetic traits in plants
Abdullah Makhzoum
Department of Biological Sciences and Biotechnology, Botswana International University of Science and Technology, Palapye, Botswana; Correspondenceabmakhzoum@gmail.com
Hindex: 10
-
New Cas9 Variants and other nucleases broadening the scope of CRISPR Toolbox Dr. Goetz Hensel
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Plant Reproductive Biology
Corrensstr. 3
D-06466 Seeland/OT Gatersleben
GERMANY
Email: hensel@ipk-gatersleben.de
Hindex: 33
-
Transgene free genome editing in plants using CRISPR/CAS9 Dr. Niaz Ahmad Dr. Penny Hundleby Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK
Email: penny.hundleby@jic.ac.uk
Prof. Mehboob Rahman
Group Leader Plant genomics & Mol. Breeding Lab, National Institute for Biotechnology & Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan.
Email: mehboob_pbd@yahoo.com
H index 23
Genome editing in plants for improvement of resistance in plants against fungal and viral pathogens Dr. Kathleen Hefferon
Food Science and Technology, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA
Email: klh22@cornell.edu H index 14
-
Characterization of gene edited crops via metabolomics. Dr. Muhammad Qudrat Ullah Farooqi
School of Agriculture and Environment; Faculty of Science
The University of Western Australia, Perth WA 6009 Australia
Email: muhammadqudratullah.farooqi@uwa.edu.au
-
Genome editing in plants for improvement of resistance in plants against bacterial pathogen Dr. Rajesh Mehrotra
Birla Institute of Technology and Science (BITS-Pilani), Rahashthan, India
Email:
rmehrotra@pilani.bits-pilani.ac.in
Dr. Purvalohan Bhalothia
Email: purvalohan@gmail.com
H index 16
-
Improvement of resistance in plants against Insect-pest using genome editing tools Dr Sandeep Kumar,
Assistant Professor, Department of Zoology,
KU SSJ Campus, Almora, Uttarakhand,India
Email: sandeep.ento@gmail.com Comparing the efficiency of different delivery methods of CRISPR/CAS9 toolkits into plants Dr Ananda Sarkar
National Institute of Plant Genome Research (NIPGR), Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi, Delhi, 110067, India
Email:aksarkar@nipgr.ac.in
Hindex 17
-
Recent trends in targeting genome editing of tomato for abiotic stress tolerance Dr. P. Hima Kumari, Jawaharlal Nehru Technological University, Hyderabad, 500085 phimakumari@gmail.com
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Genome editing approaches for improving nitrogen use efficiency and deciphering mineral nutrient homeostasis Viswanathan Chinnusamy Principal Scientist & Head Division of Plant Physiology
ICAR-Indian Agricultural Research Institute, New Delhi, India Email: viswa_iari@hotmail.com
Hindex : 37
-
Recent advances and application of CRISPR base editors for improvement of various traits in crops Dr Seema Pradhan and Dr Ajay Parida,
Director,
Institute of Life Sciences, Bhubaneswar, India
Email: drajayparida@gmail.com, director@ils.res.in
Dr. Seema Pradhan Email: seemapradhan3@gmail.com
-
CRISPR/Cas9-mediated targeted mutagenesis in medicinal plants Dr. Rohit Joshi Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India Email: joshirohit6@gmail.com
H-index: 17
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Biosafety issue related to genome editing in plants using CRISPR-Cas9 Dr. Ramesh Katam Department of Biological Sciences, Florida A&M University, Tallahassee, FL 32307, USA Email: ramesh.katam@gmail.com - 22
IPR and regulatory issues in Genome editing in plants. Dimple Sharma
Plant and Soil Science department
Texas Tech University, USA Email: dimple.sharma@ttu.edu
