Description
Book SynopsisThis detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies.
Table of ContentsPart I: Extracellular Vesicles Detection and Isolation
1. Aptasensors for Cancerous Exosome Detection
Jin Li, Sitao Xie, Fengli Qu, and Weihong Tan
2. Detection of Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor
Lizhou Xu and Khuloud T. Al-Jamal
3. Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography
Kaloyan Takov, I-Jou Teng, and Manuel Mayr
4. Isolation and Proteomic Analysis of Mouse Serum Small Extracellular Vesicles for Individual Subject Analysis
Federica Anastasi, Marialaura Dilillo, Davide Pellegrini, and Liam A. McDonnell
5. Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytometry
Najat Hajji, Chi M. Hau, Rienk Nieuwland, and Edwin van der Pol
Part II: Isolation and Characterization of Tissue and Biofluid-Specific EVs
6. Targeted Mass Spectrometry-Based Proteomics Method to Quantify Placental Extracellular Vesicles
Andrew Lai, Carlos Palma, Alexis Salas, Flavio Carrion, and Carlos Salomon
7. Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells
Qiang Wu, Min-Xia Ke, and Huang-Tian Yang
8. Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery
Lucas Trevisan França de Lima, Juliana Müller Bark, Mohammad Rasheduzzaman, Chameera Ekanayake Weeramange, and Chamindie Punyadeera
9. Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development with Reference to MISEV Compliance
Marlene Reithmair, Anja Lindemann, Veronika Mussack, and Michael W. Pfaffl
Part III: Analysis of EV Components
10. Direct Detection of Extracellular Vesicle miRNAs Using a Single-Step RT-qPCR Assay
Ayyanar Sivanantham, Heedoo Lee, and Yang Jin
11. Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles
Anton B. Iliuk
12. Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum
Yuchen Sun, Kosuke Saito, and Yoshiro Saito
Part IV: EV Engineering
13. Extracellular Vesicles and their Use as Vehicles of Immunogens
Chiara Chiozzini, Barbara Ridolfi, and Maurizio Federico
14. Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells
Noelle Leary, Sarina Walser, and Lothar C. Dieterich
15. Generation, Characterization, and Count of Fluorescent Extracellular Vesicles
Flavia Ferrantelli, Valentina Tirelli, Valeria Barreca, and Francesco Manfredi
16. Optimized Protocol for Plasma-Derived Extracellular Vesicles Loading with Synthetic miRNA Mimic Using Electroporation
Margherita A.C. Pomatto, Federica Negro, and Giovanni Camussi
17. Extracellular Vesicle Loading via pH-Gradient Modification
Stephanie M. Kronstadt, Steven M. Jay, and Anjana Jeyaram
