Description

Book Synopsis
The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.

Table of Contents
01. General procedures. 02. Nucleic acids and E.coli. 03. Enzymes. 04. Cloning. 05. DNA/RNA separation. 06. Proteins. 07. Plant biotechnology.

Experimental Biotechnology: Practical Manual

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    A Hardback by Sunita Dutta,, Abhijit Dutta & Ashok Kumar Choudhary

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      Publisher: New India Publishing Agency
      Publication Date: Publication Date: 15/09/2011
      ISBN13: 9789380235721, 978-9380235721
      ISBN10: 9380235720
      Also in:
      Biotechnology

      Description

      Book Synopsis
      The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.

      Table of Contents
      01. General procedures. 02. Nucleic acids and E.coli. 03. Enzymes. 04. Cloning. 05. DNA/RNA separation. 06. Proteins. 07. Plant biotechnology.

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