Description
Book SynopsisThe story of microscopy over the years is one of wonder, revelation, and even love. What better words could there be to describe the amazing things that we have been able to see, learn and accomplish thanks to the progress made in this field? A love story between a pieace of glass and the rainbow with an original soundtrack mad of poetry and music.
From Galilei’s initial foray into basic optical microscopy, including the Camillo Golgi and Giuliano Toraldo di Francia lessons, to such later developments as time-resolved microscopy, multi-photon microscopy and three-dimensional microscopy to innovations such as optical nanoscopy, bioimaging and super resolution imaging, the book seeks to take the reader, be they scientist or layperson, on a journey through the evolution of the microscope and its many uses, including in the field of medicine.
The author uses visible light as a through-line to unite the various chapters, as well as using fluorescence as a touchpoint from which to map the changes in the science, a significant choice, as it, along with label-free approaches and the addition of artificial intelligence, form the natural environment for development of the modern multi-messenger microscope towards bioimaging at the nanoscale.
Table of Contents1. A Curious premise
"My grandma was a beautiful woman...". This chapter tells about the motivation to decide to do research in life and why with the optical microscope.
2. Just observe!
The optical microscope to observe living systems, from organs to proteins. The challenge from its invention to "tomorrow" to decipher cancer and neurological disorders.
3.The colours of the rainbow
We all live under the rainbow, colours are delivering the energy needed to explore the living by watching.
4. The sharpener of the light
When a curved piece of glass meets the light allows to see those fine dietails hidden to the eyes.
5. A three-dimensional world
Flatlandia is a novel, the real world is developed along three spatial dimensions and the optical microscope can produce three-dimensional animated "postcard" by simply changing the lens focus when observing around.
6. Modern times: the space and time of observations
Time is the fourth dimension that increases the budget of information at our disposal to understand what's going on at different time time and space scales.
7. Two photon are better than one
Quantum mechanics allows to start a joyful revolution in optical microscopy with relevant implicantions in medicine and biology. Two photon is a unique entity.
8. Super eyes to see beyond physical limits
Laws of physics limit the perfomances of the light microscope. No doubts. The image reconstrution channel has no limits if you are able to add information and the optical microscope an unlimited super power to visualize details.
9. Without a net
Now is time to remove the net. We are skilled enough. So lets control the shape of light to get information without fluorescent labes.
10. The liquid microscope of the future
Illumination produces multiple messages tuning across time and space scales and artificial intelligence can merge them to deciphering nature. Liquid tunable microscopy could provide the opportunity to see things differently and to change our point of view, abandoning the obsession of representing the “real world” we have in mind when forming an image. Lets see further!
11. Pop microscopy
"Grown-ups never understand anything on their own, and it is tiring for children to always have to give them explanations. "We use nice images to bring you to instruments and applications like in a pop song that people whistle in the shower.
12. Acknowledgments
It is a love narration in the love story between a curved piece of glass and the rainbow.
