Description

Book Synopsis


Trade Review
The second edition of Equine Reproductive Procedures is an expanded and comprehensive resource for veterinary clinicians, veterinary students, and reproductive specialists. It is authored by multiple respected international experts in theriogenology and equine reproductive clinical practice….All chapters are clear, concise, and descriptive, with well-annotated and supplementary images and diagrams. The book is appropriately priced and is a recommended addition to the reference library of all practitioners of equine reproduction.” - JAVMA | SEP 15, 2021 | VOL 259 | NO. 6

Table of Contents

List of Contributors xiii

Preface xv

Part I Non-Pregnant Mare 1

1 Reproductive Evaluation 3
Patrick M. McCue

2 Teasing 7
Patrick M. McCue

3 Tail Wrap and Preparation/Washing of the Perineum 11
John J. Dascanio

4 Placement of a Tail Rope 17
John J. Dascanio

5 Perineal Conformation Evaluation 21
John J. Dascanio

6 Removal of a Persistent Hymen 25
John J. Dascanio

7 Palpation of the Reproductive Tract 27
Patrick M. McCue

8 Ultrasound Evaluation 31
Patrick M. McCue

9 Prediction of Ovulation 37
Patrick M. McCue

10 Speculum Examination of the Vagina 41
John J. Dascanio

11 Digital Examination of the Vagina/Cervix 45
Sofie Sitters

12 Uterine Culture Collection: Swab/Brush 49
John J. Dascanio

13 Antimicrobiotic Sensitivity Testing 53
Jillian Bishop and Patrick M. McCue

14 Microbiology: Microbial Culture 5
Patrick M. McCue and Jillian Bishop

15 Microbiology: Gram Stain 59
Jillian Bishop and Patrick M. McCue

16 qPCR Assay for the Diagnosis of Infectious Endometritis 61
Ryan A. Ferris and Patrick M. McCue

17 Uterine Cytology Collection: Swab/Brush 67
John J. Dascanio and Ryan A. Ferris

18 Uterine Culture/Cytology: Low Volume Lavage 73
John J. Dascanio

19 Endometrial Biopsy 77
Patrick M. McCue

20 Hysteroscopic Examination of the Uterus 81
Patrick M. McCue

21 Endometrial Cyst Removal 85
John J. Dascanio

22 Chromosomal Analysis 89
Terje Raudsepp

23 Endocrinological Examination 97
Patrick M. McCue

24 Laparoscopic Examination of the Female Reproductive Track with Ovarian Biopsy 99
Dean A. Hendrickson

25 Evaluation of the Mammary Gland 103
Patrick M. McCue

26 Antisperm Antibody Testing 107
Patrick M. McCue

27 Starch Granule Test for the Evaluation of Oviductal Patency 109
Sofie Sitters and John J. Dascanio

28 Fluorescent Microspheres Test for Evaluation of Oviductal Patency 111
Sofie Sitters and John J. Dascanio

29 Oviductal Flush Procedure for the Evaluation of Oviductal Patency 113
Sofie Sitters and Patrick M. McCue

30 Prostaglandin E2 Treatment for Blocked Oviducts 117
Patrick M. McCue, Sofie Sitters and Charles F. Scoggin

31 Prostaglandin E1 Treatment for Blocked Oviducts 119
Patrick M. McCue and Charles F. Scoggin

32 Hysteroscopic Hydrotubation of the Oviducts 121
Charles F. Scoggin

33 Transvaginal Ovarian Biopsy 123
Claire E. Card

34 Uterine Lavage 127
John J. Dascanio

35 Uterine Infusion 131
John J. Dascanio

36 Therapeutics for Infectious Endometritis 133
Ryan A. Ferris

37 Bacterial Biofilm and Endometritis 137
Ryan A. Ferris

38 Latent Uterine Bacterial Infections 141
Morten Rønn Petersen and Anders Miki Bojesen

39 Acupuncture in Mare Reproduction 143
Kristina Lu and Lauren Javernick

40 Restraint for Breeding 149
John J. Dascanio

41 Natural Service: Pasture Breeding 151
Patrick M. McCue

42 Natural Service: In-hand Breeding 153
Charles F. Scoggin

43 Breeding Stitches 157
Charles F. Scoggin

44 Reinforcement Breeding 159
Charles F. Scoggin

45 Breeding with Fresh or Cooled Semen 161
John J. Dascanio and Patrick M. McCue

46 Insemination Through a Vaginal Speculum 165
Patrick M. McCue

47 Breeding with Frozen Semen 167
John J. Dascanio

48 Deep Horn Insemination 171
John J. Dascanio

49 Hysteroscopic (Low Dose) Insemination 173
Patrick M. McCue

50 Transvaginal Oocyte Pickup 177
Jennifer N. Hatzel

51 Packaging and transport of oocytes 183
Jennifer N. Hatzel

52 Intracytoplasmic Sperm Injection 187
Jennifer N. Hatzel and JoAnne Stokes

53 Caslick Operation or Vulvoplasty 191
John J. Dascanio

54 Contagious Equine Metritis Testing 195
John J. Dascanio

55 Management of Seasonal Anestrus: Artificial Photoperiod 199
Patrick M. McCue

56 Management of Seasonal Anestrus: Hormone Therapy 201
Patrick M. McCue

57 Hormone Therapy in Cycling Mares 205
Patrick M. McCue

58 Estrus Suppression 211
Patrick M. McCue

59 Intrauterine Devices for Estrus Suppression 215
Carlos M. Gradil

60 Immunocontraception 221
Patrick M. McCue

61 Harvesting and Shipping Ovaries for Oocyte Recovery 223
Patrick M. McCue

Part II Pregnant Mare 225

62 Embryo Collection 227
Patrick M. McCue

63 Embryo Evaluation 231
Patrick M. McCue

64 Embryo Biopsy 235
Patrick M. McCue

65 Embryo Packaging for Cooled Transport 237
Patrick M. McCue

66 Embryo Cryopreservation 241
Patrick M. McCue

67 Non-Surgical Embryo Transfer 245
Patrick M. McCue

68 Autogenous Transfer of ICSI-Derived Embryos 249
Jennifer N. Hatzel

69 Palpation per Rectum of the Pregnant Reproductive Tract 253
Sofie Sitters

70 Ultrasound Examination of the Pregnant Reproductive Tract 257
Patrick M. McCue

71 Speculum Examination of the Pregnant Mare 263
Sofie Sitters

72 Determination of Fetal Sex at 55–200 days 265
Richard D. Holder

73 Assessment of Late-Term Fetal Well-being 273
John J. Dascanio

74 Combined Thickness of the Uterus and Placenta 277
Patrick M. McCue

75 Placentitis: Diagnosis and Treatment 281
Justin W. McNaughten and Margo L. Macpherson

76 Doppler Ultrasonography 287
John J. Dascanio

77 Prediction of Fetal Age 291
John J. Dascanio

78 Twin Reduction: Manual Technique 295
John J. Dascanio

79 Twin Reduction: Transvaginal Aspiration 299
John J. Dascanio

80 Twin Reduction: Cranio-Cervical Dislocation 303
Karen Wolfsdorf

81 Twin Reduction: Thoracic Compression 307
Patrick M. McCue

82 Twin Reduction: Transabdominal Fetal Cardiac Puncture 309
John J. Dascanio

83 Elective Termination of Pregnancy 313
John J. Dascanio

84 Abortion Diagnostic Evaluation: Sample Collection in the Field and Submission 317
Julie T. Cecere and John J. Dascanio

85 Endocrine Evaluation of Pregnancy 319
Patrick M. McCue

86 Treatment of Vaginal Varicosities 323
John J. Dascanio

87 Manual Correction of Uterine Torsion 327
John J. Dascanio

88 Preparation for Foaling 331
John J. Dascanio

89 Assessment of Mammary Gland Secretions to Predict Foaling 335
John J. Dascanio

90 Assessment of pH of Mammary Gland Secretions to Predict Foaling 339
Patrick M. McCue

91 Monitoring of Peri-Parturient Mares Using Video and Web Cameras 343
Ryan A. Ferris

92 Foaling Alert: Vulvar Device 345
John J. Dascanio

93 Positional Labor Alert Devices 347
Ryan A. Ferris

94 Induction of Parturition 351
John J. Dascanio

95 Normal Foaling 355
John J. Dascanio

96 Premature Separation of the Placenta 361
Patrick M. McCue

97 Dystocia Management 365
Patrick M. McCue and Sofie Sitters

98 Dystocia Correction 369
John J. Dascanio

99 Fetotomy 375
John J. Dascanio

100 Epidural 381
John J. Dascanio

101 Induction of Lactation to Create a Nurse Mare 383
Pouya Dini and Peter F. Daels

102 Screening to Prevent Neonatal Isoerythrolysis 385
Patrick M. McCue

Part III Postpartum Mare 387

103 Evaluation of Colostrum Specific Gravity 389
Patrick M. McCue

104 Evaluation of Colostrum Quality: Brix Refractometry 393
Patrick M. McCue

105 Colostrum Banking 395
Patrick M. McCue

106 Obtaining Milk from the Mare 399
John J. Dascanio

107 Placental Evaluation 401
Patrick M. McCue

108 Removal of Retained Placenta 407
John J. Dascanio

109 Abdominocentesis 413
John J. Dascanio

110 Uterine Prolapse Treatment 415
John J. Dascanio

111 Buhner Needle Placement of a Perivulvar Suture 419
John J. Dascanio

Part IV Stallion 423

112 Breeding Soundness Evaluation 425
Patrick M. McCue

113 Training the Stallion to Use a Phantom 429
John J. Dascanio

114 Missouri Artificial Vagina 433
John J. Dascanio and Lynda M. J. Miller

115 Colorado Model Artificial Vagina 437
Patrick M. McCue

116 Roanoke Artificial Vagina 441
John J. Dascanio

117 Hannover Artificial Vagina 445
Sofie Sitters

118 Teaser Stallions 447
Charles F. Scoggin

119 Standing Semen Collection 449
John J. Dascanio

120 Collection of Semen Using an Open-Ended Artificial Vagina 453
Patrick M. McCue

121 Chemical Ejaculation 455
Patrick M. McCue

122 Preparation of a Jump Mare or a Mare for Natural Cover 457
John J. Dascanio

123 Washing the Penis 459
John J. Dascanio

124 Evaluation of Sexual Behavior 463
John J. Dascanio and Sue M. McDonnell

125 Enhancement of Sexual Interest and Response 467
Sue M. McDonnell

126 Management of Ejaculation Difficulty 471
Sue M. McDonnell

127 Calibrated Spectrophotometer Evaluation of Sperm Concentration 475
John J. Dascanio

128 Hemocytometer Evaluation of Sperm Concentration 479
John J. Dascanio

129 NucleoCounter® Evaluation of Sperm Concentration and Viability 483
Patrick M. McCue

130 Visual Evaluation of Sperm Motility 489
Patrick M. McCue

131 Computer-Assisted Sperm Analysis 491
John J. Dascanio and Lynda M. J. Miller

132 Eosin-Nigrosin Staining in the Evaluation of Sperm 495
Julie T. Cecere

133 Evaluation of Sperm Morphology 499
Leonardo Brito

134 Determination of Daily Sperm Output 505
Patrick M. McCue

135 Measurement of Testicular Size and Estimation of Daily Sperm Output 507
Patrick M. McCue

136 Wet Mount Evaluation of Sperm 511
John J. Dascanio

137 Diff-Quik® Evaluation of Round Cells and Sperm 513
John J. Dascanio and Lynda M. J. Miller

138 Electron Microscopy of Constituents of Seminal Ejaculates 515
D. N. Rao Veeramachaneni

139 Bacterial Culture 519
John J. Dascanio

140 Evaluation of pH and Osmolarity of Semen 521
Patrick M. McCue

141 Alkaline Phosphatase: A Marker for Ejaculation 523
Patrick M. McCue

142 Diagnosis and Management of Urospermia 525
John J. Dascanio

143 Hemospermia 529
Patrick M. McCue

144 Semen Extenders and Sperm Media 533
Patrick M. McCue

145 Preparation of Semen for Cooled Transport 537
Etta A. Bradecamp

146 Packing Semen for Cooled Transport 541
Etta A. Bradecamp

147 Longevity Testing of Sperm 547
John J. Dascanio

148 Calculation of g Force for Centrifuging Semen 549
John J. Dascanio

149 Centrifugation of Semen: Standard Technique 551
Etta A. Bradecamp

150 Centrifugation of Semen: Cushion Technique 557
Etta A. Bradecamp

151 Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique 561
Etta A. Bradecamp

152 Use of a SpermFilter 563
Marco A. Alvarenga and Lorenzo G. T. M. Segabinazzi

153 Semen Freezing 567
Patrick M. McCue

154 Refreezing Semen 573
Patrick M. McCue

155 Thawing Frozen Semen 577
John J. Dascanio

156 Semen Processing for Intracytoplasmic Sperm Injection 581
Patrick M. McCue

157 Preparing a Vapor Shipper 585
Julie T. Cecere and Paul Loomis

158 Loading a Vapor Shipper 589
Julie T. Cecere and Paul Loomis

159 Maintaining a Long-Term Frozen Semen Storage Tank 591
John J. Dascanio

160 International Transport of Frozen Semen 593
Paul Loomis

161 Harvesting and Shipping Testes 597
John J. Dascanio

162 Epididymal Sperm Recovery 599
John J. Dascanio and Lynda M. J. Miller

163 Endoscopic Examination of the Urethra 603
Patrick M. McCue

164 Endoscopic-Guided Cannulation of the Seminal Vesicles 607
Patrick M. McCue

165 Palpation and Ultrasonography of the Testis, Epididymis, and Spermatic Cord 609
Sofie Sitters

166 Palpation and Ultrasonography of the Accessory Sex Glands 615
Sofie Sitters

167 Radiographic Examination of the Penis 621
Patrick M. McCue

168 Testicular Biopsy and Aspiration 623
John J. Dascanio

169 Assessment of Sperm Plasma Membrane Integrity and Viability: Propidium Iodide/SYBR-14 627
Amanda I. Glazar, and Patrick M. McCue

170 Sperm Chromatin Structure Assay 629
Charles Love

171 Hypo-Osmotic Swelling Test 633
Patrick M. McCue

172 Assessment of Sperm Acrosomal Status: PE-PNA 635
Amanda I. Glazar and Patrick M. McCue

173 Assessment of Sperm Mitochondrial Function: JC–1 and Rhodamine 123 637
Amanda I. Glazar, and Patrick M. McCue

174 Equine Viral Arteritis Testing 639
G. Reed Holyoak and Udeni B. R. Balasuriya

175 Diagnostic Endocrinology: Baseline Hormone Levels 645
Patrick M. McCue

176 Diagnostic Endocrinology: GnRH Stimulation Tests 647
Patrick M. McCue

177 Diagnostic Endocrinology: hCG Stimulation Test 649
Patrick M. McCue

178 Diagnostic Endocrinology: Estrogen Conjugate Assay 653
Patrick M. McCue

179 Diagnostic Tests for Cryptorchidism 655
Patrick M. McCue

180 Probang Apparatus 659
Julie T. Cecere

181 Support Apparatus for Paraphimosis 663
John J. Dascanio

182 Equine Castration Techniques 667
Stacy Anderson

183 Stallion Acupuncture 671
Rhonda A. Rathgeber

Part V Newborn Foal 675

184 Birth Resuscitation 677
Patrick M. McCue

185 Handling and Restraint 681
Undine Christmann

186 Madigan Foal Squeeze Technique 687
John Madigan

187 Evaluation of Passive Transfer 691
Patrick M. McCue

188 Colostral Administration via Naso-Gastric Intubation 695
John J. Dascanio

189 Sedation 699
Undine Christmann

190 Intravenous Catheter Placement 701
Undine Christmann

191 Plasma Therapy in the Foal 707
Undine Christmann

192 Acupuncture in the Neonate 711
Peter Morresey

193 Routine Care 713
Patrick M. McCue

194 Foal Rejection 719
Patrick M. McCue

195 Fostering a Foal onto a Nurse Mare 723
Peter F. Daels and Pouya Dini

196 Entropion 725
John J. Dascanio

197 Umbilical Ultrasound 727
Undine Christmann

198 Management of Soft Tissue Laxity 733
Stacy Anderson

199 Management of Angular Limb Deformities 735
Stacy Anderson

200 Management of Congenital Flexural Limb Deformities 739
Stacy Anderson

Part VI Appendices 745

Appendix A Society for Theriogenology Stallion Reproductive Evaluation Form 745

Appendix B Mare Breeding Soundness Evaluation Form 749

Appendix C Formulary for Equine Reproduction 751

Appendix D Foaling Kit and Associated Equipment and Supplies 759

Index 761

Equine Reproductive Procedures

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    A Hardback by John Dascanio, Patrick McCue

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      View other formats and editions of Equine Reproductive Procedures by John Dascanio

      Publisher: John Wiley and Sons Ltd
      Publication Date: 27/05/2021
      ISBN13: 9781119555988, 978-1119555988
      ISBN10: 1119555981

      Description

      Book Synopsis


      Trade Review
      The second edition of Equine Reproductive Procedures is an expanded and comprehensive resource for veterinary clinicians, veterinary students, and reproductive specialists. It is authored by multiple respected international experts in theriogenology and equine reproductive clinical practice….All chapters are clear, concise, and descriptive, with well-annotated and supplementary images and diagrams. The book is appropriately priced and is a recommended addition to the reference library of all practitioners of equine reproduction.” - JAVMA | SEP 15, 2021 | VOL 259 | NO. 6

      Table of Contents

      List of Contributors xiii

      Preface xv

      Part I Non-Pregnant Mare 1

      1 Reproductive Evaluation 3
      Patrick M. McCue

      2 Teasing 7
      Patrick M. McCue

      3 Tail Wrap and Preparation/Washing of the Perineum 11
      John J. Dascanio

      4 Placement of a Tail Rope 17
      John J. Dascanio

      5 Perineal Conformation Evaluation 21
      John J. Dascanio

      6 Removal of a Persistent Hymen 25
      John J. Dascanio

      7 Palpation of the Reproductive Tract 27
      Patrick M. McCue

      8 Ultrasound Evaluation 31
      Patrick M. McCue

      9 Prediction of Ovulation 37
      Patrick M. McCue

      10 Speculum Examination of the Vagina 41
      John J. Dascanio

      11 Digital Examination of the Vagina/Cervix 45
      Sofie Sitters

      12 Uterine Culture Collection: Swab/Brush 49
      John J. Dascanio

      13 Antimicrobiotic Sensitivity Testing 53
      Jillian Bishop and Patrick M. McCue

      14 Microbiology: Microbial Culture 5
      Patrick M. McCue and Jillian Bishop

      15 Microbiology: Gram Stain 59
      Jillian Bishop and Patrick M. McCue

      16 qPCR Assay for the Diagnosis of Infectious Endometritis 61
      Ryan A. Ferris and Patrick M. McCue

      17 Uterine Cytology Collection: Swab/Brush 67
      John J. Dascanio and Ryan A. Ferris

      18 Uterine Culture/Cytology: Low Volume Lavage 73
      John J. Dascanio

      19 Endometrial Biopsy 77
      Patrick M. McCue

      20 Hysteroscopic Examination of the Uterus 81
      Patrick M. McCue

      21 Endometrial Cyst Removal 85
      John J. Dascanio

      22 Chromosomal Analysis 89
      Terje Raudsepp

      23 Endocrinological Examination 97
      Patrick M. McCue

      24 Laparoscopic Examination of the Female Reproductive Track with Ovarian Biopsy 99
      Dean A. Hendrickson

      25 Evaluation of the Mammary Gland 103
      Patrick M. McCue

      26 Antisperm Antibody Testing 107
      Patrick M. McCue

      27 Starch Granule Test for the Evaluation of Oviductal Patency 109
      Sofie Sitters and John J. Dascanio

      28 Fluorescent Microspheres Test for Evaluation of Oviductal Patency 111
      Sofie Sitters and John J. Dascanio

      29 Oviductal Flush Procedure for the Evaluation of Oviductal Patency 113
      Sofie Sitters and Patrick M. McCue

      30 Prostaglandin E2 Treatment for Blocked Oviducts 117
      Patrick M. McCue, Sofie Sitters and Charles F. Scoggin

      31 Prostaglandin E1 Treatment for Blocked Oviducts 119
      Patrick M. McCue and Charles F. Scoggin

      32 Hysteroscopic Hydrotubation of the Oviducts 121
      Charles F. Scoggin

      33 Transvaginal Ovarian Biopsy 123
      Claire E. Card

      34 Uterine Lavage 127
      John J. Dascanio

      35 Uterine Infusion 131
      John J. Dascanio

      36 Therapeutics for Infectious Endometritis 133
      Ryan A. Ferris

      37 Bacterial Biofilm and Endometritis 137
      Ryan A. Ferris

      38 Latent Uterine Bacterial Infections 141
      Morten Rønn Petersen and Anders Miki Bojesen

      39 Acupuncture in Mare Reproduction 143
      Kristina Lu and Lauren Javernick

      40 Restraint for Breeding 149
      John J. Dascanio

      41 Natural Service: Pasture Breeding 151
      Patrick M. McCue

      42 Natural Service: In-hand Breeding 153
      Charles F. Scoggin

      43 Breeding Stitches 157
      Charles F. Scoggin

      44 Reinforcement Breeding 159
      Charles F. Scoggin

      45 Breeding with Fresh or Cooled Semen 161
      John J. Dascanio and Patrick M. McCue

      46 Insemination Through a Vaginal Speculum 165
      Patrick M. McCue

      47 Breeding with Frozen Semen 167
      John J. Dascanio

      48 Deep Horn Insemination 171
      John J. Dascanio

      49 Hysteroscopic (Low Dose) Insemination 173
      Patrick M. McCue

      50 Transvaginal Oocyte Pickup 177
      Jennifer N. Hatzel

      51 Packaging and transport of oocytes 183
      Jennifer N. Hatzel

      52 Intracytoplasmic Sperm Injection 187
      Jennifer N. Hatzel and JoAnne Stokes

      53 Caslick Operation or Vulvoplasty 191
      John J. Dascanio

      54 Contagious Equine Metritis Testing 195
      John J. Dascanio

      55 Management of Seasonal Anestrus: Artificial Photoperiod 199
      Patrick M. McCue

      56 Management of Seasonal Anestrus: Hormone Therapy 201
      Patrick M. McCue

      57 Hormone Therapy in Cycling Mares 205
      Patrick M. McCue

      58 Estrus Suppression 211
      Patrick M. McCue

      59 Intrauterine Devices for Estrus Suppression 215
      Carlos M. Gradil

      60 Immunocontraception 221
      Patrick M. McCue

      61 Harvesting and Shipping Ovaries for Oocyte Recovery 223
      Patrick M. McCue

      Part II Pregnant Mare 225

      62 Embryo Collection 227
      Patrick M. McCue

      63 Embryo Evaluation 231
      Patrick M. McCue

      64 Embryo Biopsy 235
      Patrick M. McCue

      65 Embryo Packaging for Cooled Transport 237
      Patrick M. McCue

      66 Embryo Cryopreservation 241
      Patrick M. McCue

      67 Non-Surgical Embryo Transfer 245
      Patrick M. McCue

      68 Autogenous Transfer of ICSI-Derived Embryos 249
      Jennifer N. Hatzel

      69 Palpation per Rectum of the Pregnant Reproductive Tract 253
      Sofie Sitters

      70 Ultrasound Examination of the Pregnant Reproductive Tract 257
      Patrick M. McCue

      71 Speculum Examination of the Pregnant Mare 263
      Sofie Sitters

      72 Determination of Fetal Sex at 55–200 days 265
      Richard D. Holder

      73 Assessment of Late-Term Fetal Well-being 273
      John J. Dascanio

      74 Combined Thickness of the Uterus and Placenta 277
      Patrick M. McCue

      75 Placentitis: Diagnosis and Treatment 281
      Justin W. McNaughten and Margo L. Macpherson

      76 Doppler Ultrasonography 287
      John J. Dascanio

      77 Prediction of Fetal Age 291
      John J. Dascanio

      78 Twin Reduction: Manual Technique 295
      John J. Dascanio

      79 Twin Reduction: Transvaginal Aspiration 299
      John J. Dascanio

      80 Twin Reduction: Cranio-Cervical Dislocation 303
      Karen Wolfsdorf

      81 Twin Reduction: Thoracic Compression 307
      Patrick M. McCue

      82 Twin Reduction: Transabdominal Fetal Cardiac Puncture 309
      John J. Dascanio

      83 Elective Termination of Pregnancy 313
      John J. Dascanio

      84 Abortion Diagnostic Evaluation: Sample Collection in the Field and Submission 317
      Julie T. Cecere and John J. Dascanio

      85 Endocrine Evaluation of Pregnancy 319
      Patrick M. McCue

      86 Treatment of Vaginal Varicosities 323
      John J. Dascanio

      87 Manual Correction of Uterine Torsion 327
      John J. Dascanio

      88 Preparation for Foaling 331
      John J. Dascanio

      89 Assessment of Mammary Gland Secretions to Predict Foaling 335
      John J. Dascanio

      90 Assessment of pH of Mammary Gland Secretions to Predict Foaling 339
      Patrick M. McCue

      91 Monitoring of Peri-Parturient Mares Using Video and Web Cameras 343
      Ryan A. Ferris

      92 Foaling Alert: Vulvar Device 345
      John J. Dascanio

      93 Positional Labor Alert Devices 347
      Ryan A. Ferris

      94 Induction of Parturition 351
      John J. Dascanio

      95 Normal Foaling 355
      John J. Dascanio

      96 Premature Separation of the Placenta 361
      Patrick M. McCue

      97 Dystocia Management 365
      Patrick M. McCue and Sofie Sitters

      98 Dystocia Correction 369
      John J. Dascanio

      99 Fetotomy 375
      John J. Dascanio

      100 Epidural 381
      John J. Dascanio

      101 Induction of Lactation to Create a Nurse Mare 383
      Pouya Dini and Peter F. Daels

      102 Screening to Prevent Neonatal Isoerythrolysis 385
      Patrick M. McCue

      Part III Postpartum Mare 387

      103 Evaluation of Colostrum Specific Gravity 389
      Patrick M. McCue

      104 Evaluation of Colostrum Quality: Brix Refractometry 393
      Patrick M. McCue

      105 Colostrum Banking 395
      Patrick M. McCue

      106 Obtaining Milk from the Mare 399
      John J. Dascanio

      107 Placental Evaluation 401
      Patrick M. McCue

      108 Removal of Retained Placenta 407
      John J. Dascanio

      109 Abdominocentesis 413
      John J. Dascanio

      110 Uterine Prolapse Treatment 415
      John J. Dascanio

      111 Buhner Needle Placement of a Perivulvar Suture 419
      John J. Dascanio

      Part IV Stallion 423

      112 Breeding Soundness Evaluation 425
      Patrick M. McCue

      113 Training the Stallion to Use a Phantom 429
      John J. Dascanio

      114 Missouri Artificial Vagina 433
      John J. Dascanio and Lynda M. J. Miller

      115 Colorado Model Artificial Vagina 437
      Patrick M. McCue

      116 Roanoke Artificial Vagina 441
      John J. Dascanio

      117 Hannover Artificial Vagina 445
      Sofie Sitters

      118 Teaser Stallions 447
      Charles F. Scoggin

      119 Standing Semen Collection 449
      John J. Dascanio

      120 Collection of Semen Using an Open-Ended Artificial Vagina 453
      Patrick M. McCue

      121 Chemical Ejaculation 455
      Patrick M. McCue

      122 Preparation of a Jump Mare or a Mare for Natural Cover 457
      John J. Dascanio

      123 Washing the Penis 459
      John J. Dascanio

      124 Evaluation of Sexual Behavior 463
      John J. Dascanio and Sue M. McDonnell

      125 Enhancement of Sexual Interest and Response 467
      Sue M. McDonnell

      126 Management of Ejaculation Difficulty 471
      Sue M. McDonnell

      127 Calibrated Spectrophotometer Evaluation of Sperm Concentration 475
      John J. Dascanio

      128 Hemocytometer Evaluation of Sperm Concentration 479
      John J. Dascanio

      129 NucleoCounter® Evaluation of Sperm Concentration and Viability 483
      Patrick M. McCue

      130 Visual Evaluation of Sperm Motility 489
      Patrick M. McCue

      131 Computer-Assisted Sperm Analysis 491
      John J. Dascanio and Lynda M. J. Miller

      132 Eosin-Nigrosin Staining in the Evaluation of Sperm 495
      Julie T. Cecere

      133 Evaluation of Sperm Morphology 499
      Leonardo Brito

      134 Determination of Daily Sperm Output 505
      Patrick M. McCue

      135 Measurement of Testicular Size and Estimation of Daily Sperm Output 507
      Patrick M. McCue

      136 Wet Mount Evaluation of Sperm 511
      John J. Dascanio

      137 Diff-Quik® Evaluation of Round Cells and Sperm 513
      John J. Dascanio and Lynda M. J. Miller

      138 Electron Microscopy of Constituents of Seminal Ejaculates 515
      D. N. Rao Veeramachaneni

      139 Bacterial Culture 519
      John J. Dascanio

      140 Evaluation of pH and Osmolarity of Semen 521
      Patrick M. McCue

      141 Alkaline Phosphatase: A Marker for Ejaculation 523
      Patrick M. McCue

      142 Diagnosis and Management of Urospermia 525
      John J. Dascanio

      143 Hemospermia 529
      Patrick M. McCue

      144 Semen Extenders and Sperm Media 533
      Patrick M. McCue

      145 Preparation of Semen for Cooled Transport 537
      Etta A. Bradecamp

      146 Packing Semen for Cooled Transport 541
      Etta A. Bradecamp

      147 Longevity Testing of Sperm 547
      John J. Dascanio

      148 Calculation of g Force for Centrifuging Semen 549
      John J. Dascanio

      149 Centrifugation of Semen: Standard Technique 551
      Etta A. Bradecamp

      150 Centrifugation of Semen: Cushion Technique 557
      Etta A. Bradecamp

      151 Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique 561
      Etta A. Bradecamp

      152 Use of a SpermFilter 563
      Marco A. Alvarenga and Lorenzo G. T. M. Segabinazzi

      153 Semen Freezing 567
      Patrick M. McCue

      154 Refreezing Semen 573
      Patrick M. McCue

      155 Thawing Frozen Semen 577
      John J. Dascanio

      156 Semen Processing for Intracytoplasmic Sperm Injection 581
      Patrick M. McCue

      157 Preparing a Vapor Shipper 585
      Julie T. Cecere and Paul Loomis

      158 Loading a Vapor Shipper 589
      Julie T. Cecere and Paul Loomis

      159 Maintaining a Long-Term Frozen Semen Storage Tank 591
      John J. Dascanio

      160 International Transport of Frozen Semen 593
      Paul Loomis

      161 Harvesting and Shipping Testes 597
      John J. Dascanio

      162 Epididymal Sperm Recovery 599
      John J. Dascanio and Lynda M. J. Miller

      163 Endoscopic Examination of the Urethra 603
      Patrick M. McCue

      164 Endoscopic-Guided Cannulation of the Seminal Vesicles 607
      Patrick M. McCue

      165 Palpation and Ultrasonography of the Testis, Epididymis, and Spermatic Cord 609
      Sofie Sitters

      166 Palpation and Ultrasonography of the Accessory Sex Glands 615
      Sofie Sitters

      167 Radiographic Examination of the Penis 621
      Patrick M. McCue

      168 Testicular Biopsy and Aspiration 623
      John J. Dascanio

      169 Assessment of Sperm Plasma Membrane Integrity and Viability: Propidium Iodide/SYBR-14 627
      Amanda I. Glazar, and Patrick M. McCue

      170 Sperm Chromatin Structure Assay 629
      Charles Love

      171 Hypo-Osmotic Swelling Test 633
      Patrick M. McCue

      172 Assessment of Sperm Acrosomal Status: PE-PNA 635
      Amanda I. Glazar and Patrick M. McCue

      173 Assessment of Sperm Mitochondrial Function: JC–1 and Rhodamine 123 637
      Amanda I. Glazar, and Patrick M. McCue

      174 Equine Viral Arteritis Testing 639
      G. Reed Holyoak and Udeni B. R. Balasuriya

      175 Diagnostic Endocrinology: Baseline Hormone Levels 645
      Patrick M. McCue

      176 Diagnostic Endocrinology: GnRH Stimulation Tests 647
      Patrick M. McCue

      177 Diagnostic Endocrinology: hCG Stimulation Test 649
      Patrick M. McCue

      178 Diagnostic Endocrinology: Estrogen Conjugate Assay 653
      Patrick M. McCue

      179 Diagnostic Tests for Cryptorchidism 655
      Patrick M. McCue

      180 Probang Apparatus 659
      Julie T. Cecere

      181 Support Apparatus for Paraphimosis 663
      John J. Dascanio

      182 Equine Castration Techniques 667
      Stacy Anderson

      183 Stallion Acupuncture 671
      Rhonda A. Rathgeber

      Part V Newborn Foal 675

      184 Birth Resuscitation 677
      Patrick M. McCue

      185 Handling and Restraint 681
      Undine Christmann

      186 Madigan Foal Squeeze Technique 687
      John Madigan

      187 Evaluation of Passive Transfer 691
      Patrick M. McCue

      188 Colostral Administration via Naso-Gastric Intubation 695
      John J. Dascanio

      189 Sedation 699
      Undine Christmann

      190 Intravenous Catheter Placement 701
      Undine Christmann

      191 Plasma Therapy in the Foal 707
      Undine Christmann

      192 Acupuncture in the Neonate 711
      Peter Morresey

      193 Routine Care 713
      Patrick M. McCue

      194 Foal Rejection 719
      Patrick M. McCue

      195 Fostering a Foal onto a Nurse Mare 723
      Peter F. Daels and Pouya Dini

      196 Entropion 725
      John J. Dascanio

      197 Umbilical Ultrasound 727
      Undine Christmann

      198 Management of Soft Tissue Laxity 733
      Stacy Anderson

      199 Management of Angular Limb Deformities 735
      Stacy Anderson

      200 Management of Congenital Flexural Limb Deformities 739
      Stacy Anderson

      Part VI Appendices 745

      Appendix A Society for Theriogenology Stallion Reproductive Evaluation Form 745

      Appendix B Mare Breeding Soundness Evaluation Form 749

      Appendix C Formulary for Equine Reproduction 751

      Appendix D Foaling Kit and Associated Equipment and Supplies 759

      Index 761

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