Description

Book Synopsis


Trade Review
The second edition of Equine Reproductive Procedures is an expanded and comprehensive resource for veterinary clinicians, veterinary students, and reproductive specialists. It is authored by multiple respected international experts in theriogenology and equine reproductive clinical practice….All chapters are clear, concise, and descriptive, with well-annotated and supplementary images and diagrams. The book is appropriately priced and is a recommended addition to the reference library of all practitioners of equine reproduction.” - JAVMA | SEP 15, 2021 | VOL 259 | NO. 6

Table of Contents

List of Contributors xiii

Preface xv

Part I Non-Pregnant Mare 1

1 Reproductive Evaluation 3
Patrick M. McCue

2 Teasing 7
Patrick M. McCue

3 Tail Wrap and Preparation/Washing of the Perineum 11
John J. Dascanio

4 Placement of a Tail Rope 17
John J. Dascanio

5 Perineal Conformation Evaluation 21
John J. Dascanio

6 Removal of a Persistent Hymen 25
John J. Dascanio

7 Palpation of the Reproductive Tract 27
Patrick M. McCue

8 Ultrasound Evaluation 31
Patrick M. McCue

9 Prediction of Ovulation 37
Patrick M. McCue

10 Speculum Examination of the Vagina 41
John J. Dascanio

11 Digital Examination of the Vagina/Cervix 45
Sofie Sitters

12 Uterine Culture Collection: Swab/Brush 49
John J. Dascanio

13 Antimicrobiotic Sensitivity Testing 53
Jillian Bishop and Patrick M. McCue

14 Microbiology: Microbial Culture 5
Patrick M. McCue and Jillian Bishop

15 Microbiology: Gram Stain 59
Jillian Bishop and Patrick M. McCue

16 qPCR Assay for the Diagnosis of Infectious Endometritis 61
Ryan A. Ferris and Patrick M. McCue

17 Uterine Cytology Collection: Swab/Brush 67
John J. Dascanio and Ryan A. Ferris

18 Uterine Culture/Cytology: Low Volume Lavage 73
John J. Dascanio

19 Endometrial Biopsy 77
Patrick M. McCue

20 Hysteroscopic Examination of the Uterus 81
Patrick M. McCue

21 Endometrial Cyst Removal 85
John J. Dascanio

22 Chromosomal Analysis 89
Terje Raudsepp

23 Endocrinological Examination 97
Patrick M. McCue

24 Laparoscopic Examination of the Female Reproductive Track with Ovarian Biopsy 99
Dean A. Hendrickson

25 Evaluation of the Mammary Gland 103
Patrick M. McCue

26 Antisperm Antibody Testing 107
Patrick M. McCue

27 Starch Granule Test for the Evaluation of Oviductal Patency 109
Sofie Sitters and John J. Dascanio

28 Fluorescent Microspheres Test for Evaluation of Oviductal Patency 111
Sofie Sitters and John J. Dascanio

29 Oviductal Flush Procedure for the Evaluation of Oviductal Patency 113
Sofie Sitters and Patrick M. McCue

30 Prostaglandin E2 Treatment for Blocked Oviducts 117
Patrick M. McCue, Sofie Sitters and Charles F. Scoggin

31 Prostaglandin E1 Treatment for Blocked Oviducts 119
Patrick M. McCue and Charles F. Scoggin

32 Hysteroscopic Hydrotubation of the Oviducts 121
Charles F. Scoggin

33 Transvaginal Ovarian Biopsy 123
Claire E. Card

34 Uterine Lavage 127
John J. Dascanio

35 Uterine Infusion 131
John J. Dascanio

36 Therapeutics for Infectious Endometritis 133
Ryan A. Ferris

37 Bacterial Biofilm and Endometritis 137
Ryan A. Ferris

38 Latent Uterine Bacterial Infections 141
Morten Rønn Petersen and Anders Miki Bojesen

39 Acupuncture in Mare Reproduction 143
Kristina Lu and Lauren Javernick

40 Restraint for Breeding 149
John J. Dascanio

41 Natural Service: Pasture Breeding 151
Patrick M. McCue

42 Natural Service: In-hand Breeding 153
Charles F. Scoggin

43 Breeding Stitches 157
Charles F. Scoggin

44 Reinforcement Breeding 159
Charles F. Scoggin

45 Breeding with Fresh or Cooled Semen 161
John J. Dascanio and Patrick M. McCue

46 Insemination Through a Vaginal Speculum 165
Patrick M. McCue

47 Breeding with Frozen Semen 167
John J. Dascanio

48 Deep Horn Insemination 171
John J. Dascanio

49 Hysteroscopic (Low Dose) Insemination 173
Patrick M. McCue

50 Transvaginal Oocyte Pickup 177
Jennifer N. Hatzel

51 Packaging and transport of oocytes 183
Jennifer N. Hatzel

52 Intracytoplasmic Sperm Injection 187
Jennifer N. Hatzel and JoAnne Stokes

53 Caslick Operation or Vulvoplasty 191
John J. Dascanio

54 Contagious Equine Metritis Testing 195
John J. Dascanio

55 Management of Seasonal Anestrus: Artificial Photoperiod 199
Patrick M. McCue

56 Management of Seasonal Anestrus: Hormone Therapy 201
Patrick M. McCue

57 Hormone Therapy in Cycling Mares 205
Patrick M. McCue

58 Estrus Suppression 211
Patrick M. McCue

59 Intrauterine Devices for Estrus Suppression 215
Carlos M. Gradil

60 Immunocontraception 221
Patrick M. McCue

61 Harvesting and Shipping Ovaries for Oocyte Recovery 223
Patrick M. McCue

Part II Pregnant Mare 225

62 Embryo Collection 227
Patrick M. McCue

63 Embryo Evaluation 231
Patrick M. McCue

64 Embryo Biopsy 235
Patrick M. McCue

65 Embryo Packaging for Cooled Transport 237
Patrick M. McCue

66 Embryo Cryopreservation 241
Patrick M. McCue

67 Non-Surgical Embryo Transfer 245
Patrick M. McCue

68 Autogenous Transfer of ICSI-Derived Embryos 249
Jennifer N. Hatzel

69 Palpation per Rectum of the Pregnant Reproductive Tract 253
Sofie Sitters

70 Ultrasound Examination of the Pregnant Reproductive Tract 257
Patrick M. McCue

71 Speculum Examination of the Pregnant Mare 263
Sofie Sitters

72 Determination of Fetal Sex at 55–200 days 265
Richard D. Holder

73 Assessment of Late-Term Fetal Well-being 273
John J. Dascanio

74 Combined Thickness of the Uterus and Placenta 277
Patrick M. McCue

75 Placentitis: Diagnosis and Treatment 281
Justin W. McNaughten and Margo L. Macpherson

76 Doppler Ultrasonography 287
John J. Dascanio

77 Prediction of Fetal Age 291
John J. Dascanio

78 Twin Reduction: Manual Technique 295
John J. Dascanio

79 Twin Reduction: Transvaginal Aspiration 299
John J. Dascanio

80 Twin Reduction: Cranio-Cervical Dislocation 303
Karen Wolfsdorf

81 Twin Reduction: Thoracic Compression 307
Patrick M. McCue

82 Twin Reduction: Transabdominal Fetal Cardiac Puncture 309
John J. Dascanio

83 Elective Termination of Pregnancy 313
John J. Dascanio

84 Abortion Diagnostic Evaluation: Sample Collection in the Field and Submission 317
Julie T. Cecere and John J. Dascanio

85 Endocrine Evaluation of Pregnancy 319
Patrick M. McCue

86 Treatment of Vaginal Varicosities 323
John J. Dascanio

87 Manual Correction of Uterine Torsion 327
John J. Dascanio

88 Preparation for Foaling 331
John J. Dascanio

89 Assessment of Mammary Gland Secretions to Predict Foaling 335
John J. Dascanio

90 Assessment of pH of Mammary Gland Secretions to Predict Foaling 339
Patrick M. McCue

91 Monitoring of Peri-Parturient Mares Using Video and Web Cameras 343
Ryan A. Ferris

92 Foaling Alert: Vulvar Device 345
John J. Dascanio

93 Positional Labor Alert Devices 347
Ryan A. Ferris

94 Induction of Parturition 351
John J. Dascanio

95 Normal Foaling 355
John J. Dascanio

96 Premature Separation of the Placenta 361
Patrick M. McCue

97 Dystocia Management 365
Patrick M. McCue and Sofie Sitters

98 Dystocia Correction 369
John J. Dascanio

99 Fetotomy 375
John J. Dascanio

100 Epidural 381
John J. Dascanio

101 Induction of Lactation to Create a Nurse Mare 383
Pouya Dini and Peter F. Daels

102 Screening to Prevent Neonatal Isoerythrolysis 385
Patrick M. McCue

Part III Postpartum Mare 387

103 Evaluation of Colostrum Specific Gravity 389
Patrick M. McCue

104 Evaluation of Colostrum Quality: Brix Refractometry 393
Patrick M. McCue

105 Colostrum Banking 395
Patrick M. McCue

106 Obtaining Milk from the Mare 399
John J. Dascanio

107 Placental Evaluation 401
Patrick M. McCue

108 Removal of Retained Placenta 407
John J. Dascanio

109 Abdominocentesis 413
John J. Dascanio

110 Uterine Prolapse Treatment 415
John J. Dascanio

111 Buhner Needle Placement of a Perivulvar Suture 419
John J. Dascanio

Part IV Stallion 423

112 Breeding Soundness Evaluation 425
Patrick M. McCue

113 Training the Stallion to Use a Phantom 429
John J. Dascanio

114 Missouri Artificial Vagina 433
John J. Dascanio and Lynda M. J. Miller

115 Colorado Model Artificial Vagina 437
Patrick M. McCue

116 Roanoke Artificial Vagina 441
John J. Dascanio

117 Hannover Artificial Vagina 445
Sofie Sitters

118 Teaser Stallions 447
Charles F. Scoggin

119 Standing Semen Collection 449
John J. Dascanio

120 Collection of Semen Using an Open-Ended Artificial Vagina 453
Patrick M. McCue

121 Chemical Ejaculation 455
Patrick M. McCue

122 Preparation of a Jump Mare or a Mare for Natural Cover 457
John J. Dascanio

123 Washing the Penis 459
John J. Dascanio

124 Evaluation of Sexual Behavior 463
John J. Dascanio and Sue M. McDonnell

125 Enhancement of Sexual Interest and Response 467
Sue M. McDonnell

126 Management of Ejaculation Difficulty 471
Sue M. McDonnell

127 Calibrated Spectrophotometer Evaluation of Sperm Concentration 475
John J. Dascanio

128 Hemocytometer Evaluation of Sperm Concentration 479
John J. Dascanio

129 NucleoCounter® Evaluation of Sperm Concentration and Viability 483
Patrick M. McCue

130 Visual Evaluation of Sperm Motility 489
Patrick M. McCue

131 Computer-Assisted Sperm Analysis 491
John J. Dascanio and Lynda M. J. Miller

132 Eosin-Nigrosin Staining in the Evaluation of Sperm 495
Julie T. Cecere

133 Evaluation of Sperm Morphology 499
Leonardo Brito

134 Determination of Daily Sperm Output 505
Patrick M. McCue

135 Measurement of Testicular Size and Estimation of Daily Sperm Output 507
Patrick M. McCue

136 Wet Mount Evaluation of Sperm 511
John J. Dascanio

137 Diff-Quik® Evaluation of Round Cells and Sperm 513
John J. Dascanio and Lynda M. J. Miller

138 Electron Microscopy of Constituents of Seminal Ejaculates 515
D. N. Rao Veeramachaneni

139 Bacterial Culture 519
John J. Dascanio

140 Evaluation of pH and Osmolarity of Semen 521
Patrick M. McCue

141 Alkaline Phosphatase: A Marker for Ejaculation 523
Patrick M. McCue

142 Diagnosis and Management of Urospermia 525
John J. Dascanio

143 Hemospermia 529
Patrick M. McCue

144 Semen Extenders and Sperm Media 533
Patrick M. McCue

145 Preparation of Semen for Cooled Transport 537
Etta A. Bradecamp

146 Packing Semen for Cooled Transport 541
Etta A. Bradecamp

147 Longevity Testing of Sperm 547
John J. Dascanio

148 Calculation of g Force for Centrifuging Semen 549
John J. Dascanio

149 Centrifugation of Semen: Standard Technique 551
Etta A. Bradecamp

150 Centrifugation of Semen: Cushion Technique 557
Etta A. Bradecamp

151 Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique 561
Etta A. Bradecamp

152 Use of a SpermFilter 563
Marco A. Alvarenga and Lorenzo G. T. M. Segabinazzi

153 Semen Freezing 567
Patrick M. McCue

154 Refreezing Semen 573
Patrick M. McCue

155 Thawing Frozen Semen 577
John J. Dascanio

156 Semen Processing for Intracytoplasmic Sperm Injection 581
Patrick M. McCue

157 Preparing a Vapor Shipper 585
Julie T. Cecere and Paul Loomis

158 Loading a Vapor Shipper 589
Julie T. Cecere and Paul Loomis

159 Maintaining a Long-Term Frozen Semen Storage Tank 591
John J. Dascanio

160 International Transport of Frozen Semen 593
Paul Loomis

161 Harvesting and Shipping Testes 597
John J. Dascanio

162 Epididymal Sperm Recovery 599
John J. Dascanio and Lynda M. J. Miller

163 Endoscopic Examination of the Urethra 603
Patrick M. McCue

164 Endoscopic-Guided Cannulation of the Seminal Vesicles 607
Patrick M. McCue

165 Palpation and Ultrasonography of the Testis, Epididymis, and Spermatic Cord 609
Sofie Sitters

166 Palpation and Ultrasonography of the Accessory Sex Glands 615
Sofie Sitters

167 Radiographic Examination of the Penis 621
Patrick M. McCue

168 Testicular Biopsy and Aspiration 623
John J. Dascanio

169 Assessment of Sperm Plasma Membrane Integrity and Viability: Propidium Iodide/SYBR-14 627
Amanda I. Glazar, and Patrick M. McCue

170 Sperm Chromatin Structure Assay 629
Charles Love

171 Hypo-Osmotic Swelling Test 633
Patrick M. McCue

172 Assessment of Sperm Acrosomal Status: PE-PNA 635
Amanda I. Glazar and Patrick M. McCue

173 Assessment of Sperm Mitochondrial Function: JC–1 and Rhodamine 123 637
Amanda I. Glazar, and Patrick M. McCue

174 Equine Viral Arteritis Testing 639
G. Reed Holyoak and Udeni B. R. Balasuriya

175 Diagnostic Endocrinology: Baseline Hormone Levels 645
Patrick M. McCue

176 Diagnostic Endocrinology: GnRH Stimulation Tests 647
Patrick M. McCue

177 Diagnostic Endocrinology: hCG Stimulation Test 649
Patrick M. McCue

178 Diagnostic Endocrinology: Estrogen Conjugate Assay 653
Patrick M. McCue

179 Diagnostic Tests for Cryptorchidism 655
Patrick M. McCue

180 Probang Apparatus 659
Julie T. Cecere

181 Support Apparatus for Paraphimosis 663
John J. Dascanio

182 Equine Castration Techniques 667
Stacy Anderson

183 Stallion Acupuncture 671
Rhonda A. Rathgeber

Part V Newborn Foal 675

184 Birth Resuscitation 677
Patrick M. McCue

185 Handling and Restraint 681
Undine Christmann

186 Madigan Foal Squeeze Technique 687
John Madigan

187 Evaluation of Passive Transfer 691
Patrick M. McCue

188 Colostral Administration via Naso-Gastric Intubation 695
John J. Dascanio

189 Sedation 699
Undine Christmann

190 Intravenous Catheter Placement 701
Undine Christmann

191 Plasma Therapy in the Foal 707
Undine Christmann

192 Acupuncture in the Neonate 711
Peter Morresey

193 Routine Care 713
Patrick M. McCue

194 Foal Rejection 719
Patrick M. McCue

195 Fostering a Foal onto a Nurse Mare 723
Peter F. Daels and Pouya Dini

196 Entropion 725
John J. Dascanio

197 Umbilical Ultrasound 727
Undine Christmann

198 Management of Soft Tissue Laxity 733
Stacy Anderson

199 Management of Angular Limb Deformities 735
Stacy Anderson

200 Management of Congenital Flexural Limb Deformities 739
Stacy Anderson

Part VI Appendices 745

Appendix A Society for Theriogenology Stallion Reproductive Evaluation Form 745

Appendix B Mare Breeding Soundness Evaluation Form 749

Appendix C Formulary for Equine Reproduction 751

Appendix D Foaling Kit and Associated Equipment and Supplies 759

Index 761

Equine Reproductive Procedures

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A Hardback by John Dascanio, Patrick McCue

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    View other formats and editions of Equine Reproductive Procedures by John Dascanio

    Publisher: John Wiley and Sons Ltd
    Publication Date: 27/05/2021
    ISBN13: 9781119555988, 978-1119555988
    ISBN10: 1119555981
    Also in:
    Equine veterinary medicine

    Description

    Book Synopsis


    Trade Review
    The second edition of Equine Reproductive Procedures is an expanded and comprehensive resource for veterinary clinicians, veterinary students, and reproductive specialists. It is authored by multiple respected international experts in theriogenology and equine reproductive clinical practice….All chapters are clear, concise, and descriptive, with well-annotated and supplementary images and diagrams. The book is appropriately priced and is a recommended addition to the reference library of all practitioners of equine reproduction.” - JAVMA | SEP 15, 2021 | VOL 259 | NO. 6

    Table of Contents

    List of Contributors xiii

    Preface xv

    Part I Non-Pregnant Mare 1

    1 Reproductive Evaluation 3
    Patrick M. McCue

    2 Teasing 7
    Patrick M. McCue

    3 Tail Wrap and Preparation/Washing of the Perineum 11
    John J. Dascanio

    4 Placement of a Tail Rope 17
    John J. Dascanio

    5 Perineal Conformation Evaluation 21
    John J. Dascanio

    6 Removal of a Persistent Hymen 25
    John J. Dascanio

    7 Palpation of the Reproductive Tract 27
    Patrick M. McCue

    8 Ultrasound Evaluation 31
    Patrick M. McCue

    9 Prediction of Ovulation 37
    Patrick M. McCue

    10 Speculum Examination of the Vagina 41
    John J. Dascanio

    11 Digital Examination of the Vagina/Cervix 45
    Sofie Sitters

    12 Uterine Culture Collection: Swab/Brush 49
    John J. Dascanio

    13 Antimicrobiotic Sensitivity Testing 53
    Jillian Bishop and Patrick M. McCue

    14 Microbiology: Microbial Culture 5
    Patrick M. McCue and Jillian Bishop

    15 Microbiology: Gram Stain 59
    Jillian Bishop and Patrick M. McCue

    16 qPCR Assay for the Diagnosis of Infectious Endometritis 61
    Ryan A. Ferris and Patrick M. McCue

    17 Uterine Cytology Collection: Swab/Brush 67
    John J. Dascanio and Ryan A. Ferris

    18 Uterine Culture/Cytology: Low Volume Lavage 73
    John J. Dascanio

    19 Endometrial Biopsy 77
    Patrick M. McCue

    20 Hysteroscopic Examination of the Uterus 81
    Patrick M. McCue

    21 Endometrial Cyst Removal 85
    John J. Dascanio

    22 Chromosomal Analysis 89
    Terje Raudsepp

    23 Endocrinological Examination 97
    Patrick M. McCue

    24 Laparoscopic Examination of the Female Reproductive Track with Ovarian Biopsy 99
    Dean A. Hendrickson

    25 Evaluation of the Mammary Gland 103
    Patrick M. McCue

    26 Antisperm Antibody Testing 107
    Patrick M. McCue

    27 Starch Granule Test for the Evaluation of Oviductal Patency 109
    Sofie Sitters and John J. Dascanio

    28 Fluorescent Microspheres Test for Evaluation of Oviductal Patency 111
    Sofie Sitters and John J. Dascanio

    29 Oviductal Flush Procedure for the Evaluation of Oviductal Patency 113
    Sofie Sitters and Patrick M. McCue

    30 Prostaglandin E2 Treatment for Blocked Oviducts 117
    Patrick M. McCue, Sofie Sitters and Charles F. Scoggin

    31 Prostaglandin E1 Treatment for Blocked Oviducts 119
    Patrick M. McCue and Charles F. Scoggin

    32 Hysteroscopic Hydrotubation of the Oviducts 121
    Charles F. Scoggin

    33 Transvaginal Ovarian Biopsy 123
    Claire E. Card

    34 Uterine Lavage 127
    John J. Dascanio

    35 Uterine Infusion 131
    John J. Dascanio

    36 Therapeutics for Infectious Endometritis 133
    Ryan A. Ferris

    37 Bacterial Biofilm and Endometritis 137
    Ryan A. Ferris

    38 Latent Uterine Bacterial Infections 141
    Morten Rønn Petersen and Anders Miki Bojesen

    39 Acupuncture in Mare Reproduction 143
    Kristina Lu and Lauren Javernick

    40 Restraint for Breeding 149
    John J. Dascanio

    41 Natural Service: Pasture Breeding 151
    Patrick M. McCue

    42 Natural Service: In-hand Breeding 153
    Charles F. Scoggin

    43 Breeding Stitches 157
    Charles F. Scoggin

    44 Reinforcement Breeding 159
    Charles F. Scoggin

    45 Breeding with Fresh or Cooled Semen 161
    John J. Dascanio and Patrick M. McCue

    46 Insemination Through a Vaginal Speculum 165
    Patrick M. McCue

    47 Breeding with Frozen Semen 167
    John J. Dascanio

    48 Deep Horn Insemination 171
    John J. Dascanio

    49 Hysteroscopic (Low Dose) Insemination 173
    Patrick M. McCue

    50 Transvaginal Oocyte Pickup 177
    Jennifer N. Hatzel

    51 Packaging and transport of oocytes 183
    Jennifer N. Hatzel

    52 Intracytoplasmic Sperm Injection 187
    Jennifer N. Hatzel and JoAnne Stokes

    53 Caslick Operation or Vulvoplasty 191
    John J. Dascanio

    54 Contagious Equine Metritis Testing 195
    John J. Dascanio

    55 Management of Seasonal Anestrus: Artificial Photoperiod 199
    Patrick M. McCue

    56 Management of Seasonal Anestrus: Hormone Therapy 201
    Patrick M. McCue

    57 Hormone Therapy in Cycling Mares 205
    Patrick M. McCue

    58 Estrus Suppression 211
    Patrick M. McCue

    59 Intrauterine Devices for Estrus Suppression 215
    Carlos M. Gradil

    60 Immunocontraception 221
    Patrick M. McCue

    61 Harvesting and Shipping Ovaries for Oocyte Recovery 223
    Patrick M. McCue

    Part II Pregnant Mare 225

    62 Embryo Collection 227
    Patrick M. McCue

    63 Embryo Evaluation 231
    Patrick M. McCue

    64 Embryo Biopsy 235
    Patrick M. McCue

    65 Embryo Packaging for Cooled Transport 237
    Patrick M. McCue

    66 Embryo Cryopreservation 241
    Patrick M. McCue

    67 Non-Surgical Embryo Transfer 245
    Patrick M. McCue

    68 Autogenous Transfer of ICSI-Derived Embryos 249
    Jennifer N. Hatzel

    69 Palpation per Rectum of the Pregnant Reproductive Tract 253
    Sofie Sitters

    70 Ultrasound Examination of the Pregnant Reproductive Tract 257
    Patrick M. McCue

    71 Speculum Examination of the Pregnant Mare 263
    Sofie Sitters

    72 Determination of Fetal Sex at 55–200 days 265
    Richard D. Holder

    73 Assessment of Late-Term Fetal Well-being 273
    John J. Dascanio

    74 Combined Thickness of the Uterus and Placenta 277
    Patrick M. McCue

    75 Placentitis: Diagnosis and Treatment 281
    Justin W. McNaughten and Margo L. Macpherson

    76 Doppler Ultrasonography 287
    John J. Dascanio

    77 Prediction of Fetal Age 291
    John J. Dascanio

    78 Twin Reduction: Manual Technique 295
    John J. Dascanio

    79 Twin Reduction: Transvaginal Aspiration 299
    John J. Dascanio

    80 Twin Reduction: Cranio-Cervical Dislocation 303
    Karen Wolfsdorf

    81 Twin Reduction: Thoracic Compression 307
    Patrick M. McCue

    82 Twin Reduction: Transabdominal Fetal Cardiac Puncture 309
    John J. Dascanio

    83 Elective Termination of Pregnancy 313
    John J. Dascanio

    84 Abortion Diagnostic Evaluation: Sample Collection in the Field and Submission 317
    Julie T. Cecere and John J. Dascanio

    85 Endocrine Evaluation of Pregnancy 319
    Patrick M. McCue

    86 Treatment of Vaginal Varicosities 323
    John J. Dascanio

    87 Manual Correction of Uterine Torsion 327
    John J. Dascanio

    88 Preparation for Foaling 331
    John J. Dascanio

    89 Assessment of Mammary Gland Secretions to Predict Foaling 335
    John J. Dascanio

    90 Assessment of pH of Mammary Gland Secretions to Predict Foaling 339
    Patrick M. McCue

    91 Monitoring of Peri-Parturient Mares Using Video and Web Cameras 343
    Ryan A. Ferris

    92 Foaling Alert: Vulvar Device 345
    John J. Dascanio

    93 Positional Labor Alert Devices 347
    Ryan A. Ferris

    94 Induction of Parturition 351
    John J. Dascanio

    95 Normal Foaling 355
    John J. Dascanio

    96 Premature Separation of the Placenta 361
    Patrick M. McCue

    97 Dystocia Management 365
    Patrick M. McCue and Sofie Sitters

    98 Dystocia Correction 369
    John J. Dascanio

    99 Fetotomy 375
    John J. Dascanio

    100 Epidural 381
    John J. Dascanio

    101 Induction of Lactation to Create a Nurse Mare 383
    Pouya Dini and Peter F. Daels

    102 Screening to Prevent Neonatal Isoerythrolysis 385
    Patrick M. McCue

    Part III Postpartum Mare 387

    103 Evaluation of Colostrum Specific Gravity 389
    Patrick M. McCue

    104 Evaluation of Colostrum Quality: Brix Refractometry 393
    Patrick M. McCue

    105 Colostrum Banking 395
    Patrick M. McCue

    106 Obtaining Milk from the Mare 399
    John J. Dascanio

    107 Placental Evaluation 401
    Patrick M. McCue

    108 Removal of Retained Placenta 407
    John J. Dascanio

    109 Abdominocentesis 413
    John J. Dascanio

    110 Uterine Prolapse Treatment 415
    John J. Dascanio

    111 Buhner Needle Placement of a Perivulvar Suture 419
    John J. Dascanio

    Part IV Stallion 423

    112 Breeding Soundness Evaluation 425
    Patrick M. McCue

    113 Training the Stallion to Use a Phantom 429
    John J. Dascanio

    114 Missouri Artificial Vagina 433
    John J. Dascanio and Lynda M. J. Miller

    115 Colorado Model Artificial Vagina 437
    Patrick M. McCue

    116 Roanoke Artificial Vagina 441
    John J. Dascanio

    117 Hannover Artificial Vagina 445
    Sofie Sitters

    118 Teaser Stallions 447
    Charles F. Scoggin

    119 Standing Semen Collection 449
    John J. Dascanio

    120 Collection of Semen Using an Open-Ended Artificial Vagina 453
    Patrick M. McCue

    121 Chemical Ejaculation 455
    Patrick M. McCue

    122 Preparation of a Jump Mare or a Mare for Natural Cover 457
    John J. Dascanio

    123 Washing the Penis 459
    John J. Dascanio

    124 Evaluation of Sexual Behavior 463
    John J. Dascanio and Sue M. McDonnell

    125 Enhancement of Sexual Interest and Response 467
    Sue M. McDonnell

    126 Management of Ejaculation Difficulty 471
    Sue M. McDonnell

    127 Calibrated Spectrophotometer Evaluation of Sperm Concentration 475
    John J. Dascanio

    128 Hemocytometer Evaluation of Sperm Concentration 479
    John J. Dascanio

    129 NucleoCounter® Evaluation of Sperm Concentration and Viability 483
    Patrick M. McCue

    130 Visual Evaluation of Sperm Motility 489
    Patrick M. McCue

    131 Computer-Assisted Sperm Analysis 491
    John J. Dascanio and Lynda M. J. Miller

    132 Eosin-Nigrosin Staining in the Evaluation of Sperm 495
    Julie T. Cecere

    133 Evaluation of Sperm Morphology 499
    Leonardo Brito

    134 Determination of Daily Sperm Output 505
    Patrick M. McCue

    135 Measurement of Testicular Size and Estimation of Daily Sperm Output 507
    Patrick M. McCue

    136 Wet Mount Evaluation of Sperm 511
    John J. Dascanio

    137 Diff-Quik® Evaluation of Round Cells and Sperm 513
    John J. Dascanio and Lynda M. J. Miller

    138 Electron Microscopy of Constituents of Seminal Ejaculates 515
    D. N. Rao Veeramachaneni

    139 Bacterial Culture 519
    John J. Dascanio

    140 Evaluation of pH and Osmolarity of Semen 521
    Patrick M. McCue

    141 Alkaline Phosphatase: A Marker for Ejaculation 523
    Patrick M. McCue

    142 Diagnosis and Management of Urospermia 525
    John J. Dascanio

    143 Hemospermia 529
    Patrick M. McCue

    144 Semen Extenders and Sperm Media 533
    Patrick M. McCue

    145 Preparation of Semen for Cooled Transport 537
    Etta A. Bradecamp

    146 Packing Semen for Cooled Transport 541
    Etta A. Bradecamp

    147 Longevity Testing of Sperm 547
    John J. Dascanio

    148 Calculation of g Force for Centrifuging Semen 549
    John J. Dascanio

    149 Centrifugation of Semen: Standard Technique 551
    Etta A. Bradecamp

    150 Centrifugation of Semen: Cushion Technique 557
    Etta A. Bradecamp

    151 Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique 561
    Etta A. Bradecamp

    152 Use of a SpermFilter 563
    Marco A. Alvarenga and Lorenzo G. T. M. Segabinazzi

    153 Semen Freezing 567
    Patrick M. McCue

    154 Refreezing Semen 573
    Patrick M. McCue

    155 Thawing Frozen Semen 577
    John J. Dascanio

    156 Semen Processing for Intracytoplasmic Sperm Injection 581
    Patrick M. McCue

    157 Preparing a Vapor Shipper 585
    Julie T. Cecere and Paul Loomis

    158 Loading a Vapor Shipper 589
    Julie T. Cecere and Paul Loomis

    159 Maintaining a Long-Term Frozen Semen Storage Tank 591
    John J. Dascanio

    160 International Transport of Frozen Semen 593
    Paul Loomis

    161 Harvesting and Shipping Testes 597
    John J. Dascanio

    162 Epididymal Sperm Recovery 599
    John J. Dascanio and Lynda M. J. Miller

    163 Endoscopic Examination of the Urethra 603
    Patrick M. McCue

    164 Endoscopic-Guided Cannulation of the Seminal Vesicles 607
    Patrick M. McCue

    165 Palpation and Ultrasonography of the Testis, Epididymis, and Spermatic Cord 609
    Sofie Sitters

    166 Palpation and Ultrasonography of the Accessory Sex Glands 615
    Sofie Sitters

    167 Radiographic Examination of the Penis 621
    Patrick M. McCue

    168 Testicular Biopsy and Aspiration 623
    John J. Dascanio

    169 Assessment of Sperm Plasma Membrane Integrity and Viability: Propidium Iodide/SYBR-14 627
    Amanda I. Glazar, and Patrick M. McCue

    170 Sperm Chromatin Structure Assay 629
    Charles Love

    171 Hypo-Osmotic Swelling Test 633
    Patrick M. McCue

    172 Assessment of Sperm Acrosomal Status: PE-PNA 635
    Amanda I. Glazar and Patrick M. McCue

    173 Assessment of Sperm Mitochondrial Function: JC–1 and Rhodamine 123 637
    Amanda I. Glazar, and Patrick M. McCue

    174 Equine Viral Arteritis Testing 639
    G. Reed Holyoak and Udeni B. R. Balasuriya

    175 Diagnostic Endocrinology: Baseline Hormone Levels 645
    Patrick M. McCue

    176 Diagnostic Endocrinology: GnRH Stimulation Tests 647
    Patrick M. McCue

    177 Diagnostic Endocrinology: hCG Stimulation Test 649
    Patrick M. McCue

    178 Diagnostic Endocrinology: Estrogen Conjugate Assay 653
    Patrick M. McCue

    179 Diagnostic Tests for Cryptorchidism 655
    Patrick M. McCue

    180 Probang Apparatus 659
    Julie T. Cecere

    181 Support Apparatus for Paraphimosis 663
    John J. Dascanio

    182 Equine Castration Techniques 667
    Stacy Anderson

    183 Stallion Acupuncture 671
    Rhonda A. Rathgeber

    Part V Newborn Foal 675

    184 Birth Resuscitation 677
    Patrick M. McCue

    185 Handling and Restraint 681
    Undine Christmann

    186 Madigan Foal Squeeze Technique 687
    John Madigan

    187 Evaluation of Passive Transfer 691
    Patrick M. McCue

    188 Colostral Administration via Naso-Gastric Intubation 695
    John J. Dascanio

    189 Sedation 699
    Undine Christmann

    190 Intravenous Catheter Placement 701
    Undine Christmann

    191 Plasma Therapy in the Foal 707
    Undine Christmann

    192 Acupuncture in the Neonate 711
    Peter Morresey

    193 Routine Care 713
    Patrick M. McCue

    194 Foal Rejection 719
    Patrick M. McCue

    195 Fostering a Foal onto a Nurse Mare 723
    Peter F. Daels and Pouya Dini

    196 Entropion 725
    John J. Dascanio

    197 Umbilical Ultrasound 727
    Undine Christmann

    198 Management of Soft Tissue Laxity 733
    Stacy Anderson

    199 Management of Angular Limb Deformities 735
    Stacy Anderson

    200 Management of Congenital Flexural Limb Deformities 739
    Stacy Anderson

    Part VI Appendices 745

    Appendix A Society for Theriogenology Stallion Reproductive Evaluation Form 745

    Appendix B Mare Breeding Soundness Evaluation Form 749

    Appendix C Formulary for Equine Reproduction 751

    Appendix D Foaling Kit and Associated Equipment and Supplies 759

    Index 761

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