Description
Book SynopsisHighlighting the critical importance of enzymes in pharmaceutical and biotechnology research, Enzyme Technologies presents thorough discussions on chemical biology of enzymes, redesigning binding and catalytic specificities of enzymes, and applications of enzymes to biotechnology research in the post-genomic era.
Trade Review“The book serves as a valuable desk reference volume and describes well the key concepts of the standard and emerging enzyme technologies that together constitute some of the fundamental principles and knowledge on which drug discovery research is based.” (ChemMedChem, 1 August 2015)
Table of ContentsContributors vii
Preface ix
Part A _Enzymes – essential workhorses in pharmaceutical research 1
1 Assay Technologies for Proteases 3
Anuradha Roy, Gerald H. Lushington, James McGee, and Rathnam Chaguturu
2 Discovery and Development of Isozyme-Selective Inhibitors Involved in Lipid Metabolism 55
Taichi Ohshiro and Hiroshi Tomoda
3 Covalent Enzyme Inhibition in Drug Discovery and Development 81
Shujaath Mehdi
4 Preclinomics: Enzyme Assays and Rodent Models for Metabolic diseases 131
Wu-Kuang Yeh and Richard G. Peterson
Part B _Enzymes – indispensable tools for improving druggability 163
5 Enzymes and Targeted Activation of Prodrugs 165
Yanhui Yang, Yu Chen, Herve Aloysius, Daigo Inoyama, and Longqin Hu
6 Evolution of an Orally Active Prodrug of Gemcitabine 237
James R. McCarthy
7 Enzymatically Activated Phosphate and Phosphonate Prodrugs 253
Ivan S. Krylov and Charles E. McKenna
Part C E nzymes – powerful weapons for correcting Nature’s errors 301
8 Treatment Options for Mucopolysaccharidosis Type II (Hunter’s Syndrome) 303
Michael Beck
9 Enzyme Replacement Therapy for Fabry Disease 321
Ley Nadine Lacbawan, Wei Zheng, and Ozlem Goker-Alpan
10 Methods and Principles of Pancreatic Function Tests 335
Henrike von Schassen, Jutta Keller, and Peter Layer
Index 341
