Description
Book SynopsisThis book details the current and future tools used in the production of bulk chemicals and biofuels developed from renewable biomass using green technologies. It describes in depth the technology used to unravel the complexity of microbial metabolism in order to produce engineering strains at time scales much faster than would occur naturally.
Trade Review“This book should be valuable to engineers, biochemists, and students who seek to understand the science and practice of engineering biocatalysts for industrial applications.” (Chemical Engineering Progress, 1 January 2013)
Table of ContentsForeword vii
John Pierce
Preface ix
Contributors xi
1 Classical Strain Improvement 1
Nathan Crook and Hal S. Alper
2 Tracer-Based Analysis of Metabolic Flux Networks 35
Michael Dauner
3 Integration of “Omics” Data with Genome-Scale Metabolic Models 77
Stephen Van Dien, Priti Pharkya, and Robin Osterhout
4 Strain Improvement via Evolutionary Engineering 111
Byoungjin Kim, Jing Du, and Huimin Zhao
5 Rapid Fermentation Process Development and Optimization 133
Jun Sun and Lawrence Chew
6 The Clavulanic Acid Strain Improvement Program at DSM Anti-Infectives 169
Bert Koekman and Marcus Hans
7 Metabolic Engineering of Recombinant E. coli for the Production of 3-Hydroxypropionate 185
Tanya Warnecke Lipscomb, Matthew L. Lipscomb, Ryan T. Gill, and Michael D. Lynch
8 Complex System Engineering: A Case Study for an Unsequenced Microalga 201
Michael T. Guarnieri, Lieve M.L. Laurens, Eric P. Knoshaug, Yat-Chen Chou, Bryon S. Donohoe, and Philip T. Pienkos
9 Meiotic Recombination-Based Genome Shuffling of Saccharomyces Cerevisiae and Schefferomyces Stiptis for Increased Inhibitor Tolerance to Lignocellulosic Substrate Toxicity 233
Dominic Pinel and Vincent J.J. Martin
Index 251
