Description

Book Synopsis
The central purpose of this combination lecture/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Electrophoresis, Southern and Western blotting and cominatorial techniques are emphasized. Radioisotopes, fluorophores and solvent effects on protein structure are discussed. (No radioisotopes are required.) The experiments included have been selected because of their high success rate and because they emphasize a project-oriented approach. Sufficient detail and background are provided such that the intended audience includes advanced undergr

Table of Contents
INTRODUCTORY UNIT; PART I - NCULEIC ACIDS & CLONING; UNIT 1; UNIT 2; UNIT 3; UNIT 4; UNIT 5; UNIT 6; PART 2 - PROTEIN PURIFICATION; UNIT 7; UNIT 8; UNIT 9; UNIT 10; APPENDICES; INDEX

Cloning Gene Expression and Protein Purification

    Product form

    A Paperback / softback by Charles Hardin, Jennifer Pinczes, Andrew Riell

    £70.00

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      Book details

      Publisher Oxford University Press Inc
      Published 17 May 2001
      ISBN-13 9780195132946
      978-0195132946
      ISBN-10 0195132947
      Also in:
      Biochemistry Genetics (non-medical) Genetic engineering

      Description

      Book Synopsis
      The central purpose of this combination lecture/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Electrophoresis, Southern and Western blotting and cominatorial techniques are emphasized. Radioisotopes, fluorophores and solvent effects on protein structure are discussed. (No radioisotopes are required.) The experiments included have been selected because of their high success rate and because they emphasize a project-oriented approach. Sufficient detail and background are provided such that the intended audience includes advanced undergr

      Table of Contents
      INTRODUCTORY UNIT; PART I - NCULEIC ACIDS & CLONING; UNIT 1; UNIT 2; UNIT 3; UNIT 4; UNIT 5; UNIT 6; PART 2 - PROTEIN PURIFICATION; UNIT 7; UNIT 8; UNIT 9; UNIT 10; APPENDICES; INDEX

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