Description
Book SynopsisThis detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world.
Table of ContentsPart I: Establishment of Luciferins and Luciferases
1. Gene Cloning and Functional Analysis of the Luciferase from Luminous Syllids of the Genus Odontosyllis
Rie Yasuno, Yasuo Mitani, and Yoshihiro Ohmiya
2. Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging
Tianyu Jiang and Minyong Li
3. Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging
Nobuo Kitada, Shojiro Maki, and Sung-Bae Kim
4. Biosynthesis-Inspired Deracemizative Production of D-Luciferin In Vitro by Combining Luciferase and Thioesterase
Kazuki Niwa and Dai-ichiro Kato
5. Production of Metridia Luciferase in Native Form by Oxidative Refolding from E. coli Inclusion Bodies
Svetlana V. Markova, Marina D. Larionova, and Eugene S. Vysotski
6. Production of Copepod Luciferases via Baculovirus Expression System
Marina D. Larionova, Svetlana V. Markova, and Eugene S. Vysotski
7. Molecular Tension Probe for In Vitro Bioassays
Sung-Bae Kim, Rika Fujii, Simon Miller, and Mikio Tanabe
Part II: Basic In Vitro Applications
8. Optimized Loop-Mediated Amplification (LAMP) Allows Single Copy Detection Using Bioluminescent Assay in Real Time (BART)
Patrick Hardinge
9. A Simple and Rapid Bioluminescence-Based Functional Assay of Organic Anion Transporter 1 as a d-Luciferin Transporter
Katsuhisa Inoue, Koki Sugiyama, and Takahito Furuya
10. A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules against the Intracellular Form of Leishmania infantum
Diego Benítez, Andrea Medeiros, Cristina Quiroga, and Marcelo A. Comini
11. A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening against Infective African Trypanosomes
Estefania Dibello, Marcelo A. Comini, and Diego Benítez
12. Imaging of Autonomous Bioluminescence Emission from Single Mammalian Cells
Carola Gregor
13. Rapid Single-Cell Detection of Beer-contaminating Lactic Acid Bacteria Using Bioluminescence/Rapid Microbe Detection
Toshihiro Takahashi and Yasukazu Nakakita
14. Bioluminescence of Aliivibrio fischeri in Artificial Seawater and Its Application in Fungicide Sensing
Hitomi Kuwahara and Hiroshi Morita
15. A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor
Thabat Khatib, Berndt Müller, and Peter McCaffery
16. Design of an Intron-Retained Bioluminescence Reporter and Its Application in Imaging of Pre-mRNA Splicing in Living Subjects
Fu Wang, Si Chen, Haifeng Zheng, and Bin Guo
17. Generation of Bi-Reporter Expressing Tri-Segmented Arenavirus
Chengjin Ye and Luis Martinez-Sobrido
18. Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2
Desarey Morales Vasquez, Kevin Chiem, Chengjin Ye, and Luis Martinez-Sobrido
19. Generation, Characterization, and Applications of Influenza A Reporter Viruses
Kevin Chiem, Aitor Nogales, and Luis Martinez-Sobrido
Part III: Basic In Vivo Applications
20. Optimized Aequorin Reconstitution Protocol to Visualize Calcium Ion Transients in the Heart of Transgenic Zebrafish Embryos In Vivo
Manuel Vicente, Jussep Salgado-Almario, Antonio Martínez-Sielva, Juan Llopis, and Beatriz Domingo
21. Quantification and Imaging of Exosomes via Luciferase-Fused Exosome Marker Proteins: ExoLuc System
Tomoya Hikita and Chitose Oneyama
22. Bioluminescent Tracking of Human Induced Pluripotent Stem Cells In Vitro and In Vivo
Toshinobu Nishimura, Kouta Niizuma, and Hiromitsu Nakauchi
23. Noninvasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase
Yang Bi, Nannan Zhang, and Yun He
24. Bioluminescence Imaging for Evaluation of Antitumor Effect In Vitro and In Vivo in Mice Xenografted Tumor Models
Kazuhide Sato
25. Detection of Spontaneous Bone Metastases of Solid Human Tumor Xenografts in Mice
Vera Labitzky, Ursula Valentiner, and Tobias Lange
26. In Vivo Imaging Analysis of an Inner Ear Drug Delivery in Mice: Comparison of Inner Ear Drug Concentrations Over Time
Sho Kanzaki, Shinsuke Shibata, Masaya Nakamura, Masahiro Ozaki, and Hideyuki Okano
27. Protocols for the Evaluation of a Lymphatic Drug Delivery System Combined with Bioluminescence to Treat Metastatic Lymph Nodes
Ariunbuyan Sukhbaatar and Tetsuya Kodama
28. In Vivo Bioluminescent Imaging of Rabies Virus Infection and Evaluation of Antiviral Drug
Kentaro Yamada and Akira Nishizono
29. Imaging Infection by Vector-Borne Protozoan Parasites Using Whole-Mouse Bioluminescence
Mónica Sá, David Mendes Costa, and Joana Tavares
30. Longitudinal Tracing of Lyssavirus Infection in Mice via In Vivo Bioluminescence Imaging
Kate E. Mastraccio, Celeste Huaman, Eric D. Laing, Christopher C. Broder, and Brian C. Schaefer
Part IV: Multiplex Imaging Platforms
31. Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Anti-Relapse Compounds
Annemarie M. Voorberg-van der Wel, Anne-Marie Zeeman, Ivonne G. Nieuwenhuis, Nicole M. van der Werff, and Clemens H. M. Kocken
32. Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase Reporter Plasmids
Alejandro Sarrion-Perdigones, Yezabel Gonzalez, and Koen J.T. Venken
33. Multiplex Hextuple Luciferase Assaying
Alejandro Sarrion-Perdigones, Yezabel Gonzalez, Lyra Chang, Tatiana Gallego-Flores, Damian W. Young, and Koen J.T. Venken
34. Molecular Imaging of Tumor Progression and Angiogenesis by Dual Bioluminescence
Yue Liu, Ziyu Huang, and Zongjin Li
