Description
Book SynopsisThis textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
Table of ContentsPart I: Chapter 1: A brief history of protein engineeringChapter 2: From DNA to functional protein – A refresherChapter 3: Basic laboratory and computational techniques
Part II:Chapter 4: Protein engineering strategiesChapter 5: Computational tools for protein engineeringChapter 6: Gene isolationChapter 7: Rational design Chapter 8: Directed evolutionChapter 9: Protein modification
Part III:Chapter 10: Career prospect of a protein engineerChapter 11: Conversations with protein engineers
