Description

Book Synopsis


Table of Contents

Chemical Screening in Zebrafish.- TALEN-Mediated Mutagenesis and Genome Editing.- Homology Independent Integration of Plasmid DNA into the Zebrafish Genome.- Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System.- Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish.- Testing of cis-Regulatory Elements by Targeted Transgene Integration in Zebrafish using PhiC31 Integrase.- Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP Positive Cells from Transgenic Zebrafish Lines.- Construction of the Inbred Strain.- Zebrafish as a Model for the Study of Solid Malignancies.- Melanoma Regression and Recurrence in Zebrafish.- Imaging of Human Cancer Cell Proliferation, Invasion and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model.- Modeling Leukemogenesis in the Zebrafish using Genetic and Xenograft Models.- Enumerating Hematopoietic Stem and Progenitor Cells in Zebrafish Embryos.- Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.- Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish.- Studying Lipid Metabolism and Transport During Zebrafish Development.- Targeted Electroporation in Embryonic, Larval and Adult Zebrafish.- Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy.- In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain.- Quantifying Aggressive Behaviour in Zebrafish.- Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish.- A Practical Guide to Light Sheet Microscopy.- Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish.- Fiber Optic-Based Photostimulation of Larval Zebrafish.- Genetic Ablation, Sensitization and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels.

Zebrafish Methods and Protocols Methods in

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    A Hardback by Koichi Kawakami, E. Elizabeth Patton, Michael Orger

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      Publisher: Humana Press Inc.
      Publication Date: Publication Date: 27/07/2016
      ISBN13: 9781493937691, 978-1493937691
      ISBN10: 1493937693

      Description

      Book Synopsis


      Table of Contents

      Chemical Screening in Zebrafish.- TALEN-Mediated Mutagenesis and Genome Editing.- Homology Independent Integration of Plasmid DNA into the Zebrafish Genome.- Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System.- Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish.- Testing of cis-Regulatory Elements by Targeted Transgene Integration in Zebrafish using PhiC31 Integrase.- Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP Positive Cells from Transgenic Zebrafish Lines.- Construction of the Inbred Strain.- Zebrafish as a Model for the Study of Solid Malignancies.- Melanoma Regression and Recurrence in Zebrafish.- Imaging of Human Cancer Cell Proliferation, Invasion and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model.- Modeling Leukemogenesis in the Zebrafish using Genetic and Xenograft Models.- Enumerating Hematopoietic Stem and Progenitor Cells in Zebrafish Embryos.- Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae.- Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish.- Studying Lipid Metabolism and Transport During Zebrafish Development.- Targeted Electroporation in Embryonic, Larval and Adult Zebrafish.- Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy.- In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain.- Quantifying Aggressive Behaviour in Zebrafish.- Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish.- A Practical Guide to Light Sheet Microscopy.- Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish.- Fiber Optic-Based Photostimulation of Larval Zebrafish.- Genetic Ablation, Sensitization and Isolation of Neurons Using Nitroreductase and Tetrodotoxin-Insensitive Channels.

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