Description
Book SynopsisThis volume provides researchers with the most updated understanding of the basics of West Nile Virus (WNV). Chapters focus on the biology, virology, epidemiology, pathogenesis, as well as the step-by-step molecular, cellular, and statistical methods to study WNV infection in cell culture, mosquitos, animal models, and human clinical specimen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, West Nile Virus: Methods and Protocols aims to be a valuable resource for all researchers interested in learning more about this important and developing field.
Table of Contents1. Introduction to West Nile Virus
Shazeed-Ul Karim and Fengwei Bai
2. Quantification of West Nile virus by Plaque-Forming Assay
Biswas Neupane and Fengwei Bai
3. Viral titer quantification of West Nile virus by immunostaining plaque assay
Na Li, Cheng-Lin Deng, Bo Zhang and Han-Qing Ye
4. Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection
Farzana Nazneen and Fengwei Bai
5. Isolation of Murine Bone Marrow Derived Neutrophils for Infection Modeling
Laurel Duty and Amber M. Paul
6. Methods to study West Nile Virus infection, and the virus-induced inflammation in the brain in a murine model
Huanle Luo and Tian Wang
7. Detection of West Nile virus envelope protein in brain tissue with an immunohistochemical assay
Kathleen T. Yee and Douglas E. Vetter
8. Quantitative Analysis of B Cell Subpopulations in Bone Marrow by Flow Cytometry
Tingting Geng, Penghua Wang
9. Isolation of exosomes or extracellular vesicles from West Nile Virus-infected
N2a cells, Primary Cortical Neurons and Brain Tissues
Hameeda Sultana and Girish Neelakanta
10. Protocol to Study West Nile Virus Infection in Brain Slices In Vitro
Parminder J.S. Vig
11. Studying Virus-Host Interactions with CRISPR technology
Rong Zhang
12. Protocol of detection of West Nile Virus in Clinical Samples
Hephzibah Nwanosike, Freedom M. Green, Kristy O. Murray, Jill E. Weatherhead, and Shannon E. Ronca
13. Detection and Analysis of West Nile Virus Structural Protein Genes in Animal Or Bird Samples
Gili Schvartz, Sharon Karniely, Roberto Azar, Areej Kabat, Amir Steinman, and Oran Erster
14. Mosquito surveillance for West Nile Virus
Donald A. Yee, Ary Faraji, and Ilia Rochlin
15. Molecular surveillance of West Nile virus in mosquitoes and sentinel chickens
Steven T Peper
16. Statistical tools for West Nile Virus Disease Analysis
Mathew J Ward, Meytar Sorek-Hamer, Krishna Vemur, and Nicholas B DeFelice
17. Analytical Approaches to Uncover Genetic Associations for Rare Outcomes: Lessons from West Nile Neuroinvasive Disease
Megan E. Cahill and Ruth R. Montgomery
18. Safety procedures to work with West Nile virus in biosafety level 3 facilities
Freedom M. Green and Shannon E. Ronca
19. Development of antibody-based therapeutics against West Nile virus in plants
Haiyan Sun, Joshua Lesio, and Qiang Chen
