Description

Book Synopsis

Poxvirus Epidemiology.- Mpox Specimen Collection, Storage, Preparation, and Laboratory Diagnostics by PCR.- A Quantitative Real-Time PCR Assay for Measuring Poxvirus Replication and Cell Binding.- Orthopoxvirus Genome Sequencing, Assembly, and Analysis.- Bioinformatics for the Structural Genomics of Poxviruses.- Construction and Isolation of Recombinant Vaccinia Virus by Homologous Recombination Using Fluorescent Protein Markers.- Rapid Generation of Recombinant Poxviruses Using CRISPR/Cas9 Gene Editing.- Cas9-Mediated Poxvirus Recombinant Recovery (CASPRR) for Fast Recovery of Recombinant Vaccinia Viruses.- Use of a Poxvirus K3 Ortholog as a Positive Selection Marker for Construction of Recombinant Vaccinia Virus with Modified Host Range.- Orthopoxvirus Purification for High Containment Laboratories.- Evaluation of Vaccinia Virus Infection in Mice Using Two-Reporter Recombinant Virus.- Bioluminescence Imaging to Study Recombinant Orthopoxvirus Infection in Animal Models.- Methods t

Vaccinia Mpox and Other Poxviruses

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    A Hardback by Zhilong Yang

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      Publisher: Humana
      Publication Date: 12/3/2024
      ISBN13: 9781071641590, 978-1071641590
      ISBN10: 107164159X
      Also in:
      Microbiology (non-medical)

      Description

      Book Synopsis

      Poxvirus Epidemiology.- Mpox Specimen Collection, Storage, Preparation, and Laboratory Diagnostics by PCR.- A Quantitative Real-Time PCR Assay for Measuring Poxvirus Replication and Cell Binding.- Orthopoxvirus Genome Sequencing, Assembly, and Analysis.- Bioinformatics for the Structural Genomics of Poxviruses.- Construction and Isolation of Recombinant Vaccinia Virus by Homologous Recombination Using Fluorescent Protein Markers.- Rapid Generation of Recombinant Poxviruses Using CRISPR/Cas9 Gene Editing.- Cas9-Mediated Poxvirus Recombinant Recovery (CASPRR) for Fast Recovery of Recombinant Vaccinia Viruses.- Use of a Poxvirus K3 Ortholog as a Positive Selection Marker for Construction of Recombinant Vaccinia Virus with Modified Host Range.- Orthopoxvirus Purification for High Containment Laboratories.- Evaluation of Vaccinia Virus Infection in Mice Using Two-Reporter Recombinant Virus.- Bioluminescence Imaging to Study Recombinant Orthopoxvirus Infection in Animal Models.- Methods t

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