Description
Book SynopsisThis book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful
Metho
Table of Contents
1. Molecular Evolution Bioinformatics towards Structural Biology of TRPV1-4 Channels
Pau Doñate-Macián, Alba Crespi-Boixader, and Alex Perálvarez-Marín
2. Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6
Appu K. Singh, Luke L. McGoldrick, and Alexander I. Sobolevsky
3. Cryo-Electron Microscopy of TRP Channels
Amrita Samanta, Taylor E.T. Hughes, and Vera Y. Moissenkova-Bell
4. Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation
Verónica I. Dumit, Michael Köttgen, and Alexis Hofherr
5. Combining Structural Data with Computational Methodologies to Investigate Structure-Function Relationships in TRP Channels
Victoria Oakes and Carmen Domene
6. Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique
Elvira de la Peña and Ana Gomis
7. Fluorescence-Based Functional Assays for Ca2+ Permeable ThermoTRP Channels
Celia Cordero-Sánchez, Irene Mudarra-Fraguas, and Asia Fernández-Carvajal
8. High Content Imaging of Immunofluorescently-Labeled TRPV1-Positive Sensory Neurons
Jörg Isensee and Tim Hucho
9. Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels
Beiying Liu and Feng Qin
10. TRP Channels Reconstitution in Lipid Bilayers
Eleonora Zakharian
11. Methods for Investigating TRP Channel Gating
Osvaldo Alvarez, Karen Castillo, Emerson Carmona, Carlos Gonzalez, and Ramon Latorre
12. In Silico Approaches for TRP Channel Modulation
Magdalena Nikolaeva Koleva and Gregorio Fernández-Ballester
13. Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction
Tracey Pirali, Ubaldina Galli, Marta Serafini, Alessia Griglio, Armando A. Genazzani, and Gian Cesare Tron
14. In Vivo Methods to Study ThermoTRP Channels in Rodents
Sara González-Rodríguez
