Description
Book SynopsisWritten by the international community's leading experts, Trace Elements in Laboratory Rodents describes the best and most current methods to provide deficient or supplemental trace elements to laboratory animals, as well as how to assay them. The experts warn of the common pitfalls and hidden problems in nutritional testing and how to avoid them. This how-to approach focuses on the technical details that make good, reliable studies. Common as well as rare or recently recognized minerals are described relating to both dietary supplementation and measurement in tissues.
If you are a researcher, professor, or student working in nutrition, food science, biochemistry, or veterinary medicine, you can't afford to be without this excellent hands-on methods manual!
Trade Review"...a 'how-to-do book' that weould be a welcome addition in any nutritional or toxicological scientist's lib rary....Ronald Watson has done a marvelous job in assembling 21 detailed chapters into a comprehensive reference....a great text for those who need a resource that focuses on the details. This book will serve as a key reference resource for the researcher, nutritionalist, toxicologist, and veterinary or animal care person. In addition, it could serve well as a supplemental book for a graduate course on nutrition and trace elements."
-Environmental Nutrition International
"Designed for the researcher with a focus on laboratory and field nutritional research."
- Ira Wolinsky, Series Editor
Table of ContentsGeneral Overview: AIN-93 Purified Diets for the Study of Trace Elements Metabolism in Rodents. Basic Tissue Preparation for Electron Microscopy Assessment of Rodents. Iron: Dietary Iron: Deficiency or Excess. The Use of Iron-Dextran to Produce Iron Overload in Rodents. Extraction and Analysis of Iron Species in Diet and Gut. Manganese: Manganese Deficiency and Excess in Rodents. Manganese Uptake in Tissues In Vitro: Tissue Slices as Models. Selenium: Selenium Diets: Deficiency and Excess. Selenium in Tissue Culture. Copper: Copper Deficient and Excess Diets: Theoretical Considerations and Preparations. Copper in Tissue Culture. Zinc: Zinc Diets: Deficiency and Excess. Zinc Radiotracer in the Study of the Mechanisms of Zinc Homeostasis. Other Trace Elements: Dietary Boron Deficiency and Supplementation. Measurements of Boron in Rodent Diets and Tissues. Methods for Studying Dietary Lead and its Toxicity in Rodents. Methods in Chromium Dietary Supplementation and Deficiency. Isolation and In Vitro Analysis of Biologically Active Chromium. Calcium Bioavailability Using a Rat Model. Vanadium Quantitation, Essentiality, and Pharmacologic and Toxicological Studies in Rodents. Nickel-Low Diet Formulation and Tissue Nickel Measurement. Index.
