Description
Book SynopsisExpression and One-Step Purification of His-tagged Bacterial Topoisomerase Subunits from Escherichia coli.- Bacterial RADAR Assay: Isolation and Detection of Intracellular Covalent Complexes.- Bacterial Topoisomerase I Growth Complementation Assay.- DNA Supercoiling Catalyzed by Bacterial Gyrase.- DNA Decatenation Catalyzed by Bacterial Topoisomerase IV.- DNA Cleavage Mediated by Bacterial Type II Topoisomerases.- Expression, purification, and measurement of DNA supercoil relaxation activity by eukaryotic topoisomerase II.- Plasmid DNA Cleavage Assay with Eukaryotic Topoisomerase II.- Topoisomerase II DNA Cleavage Assay Using Fluorescently Labeled Double-Stranded Oligonucleotides.- Plasmid DNA Binding Electrophoretic Mobility Shift Assay with Eukaryotic Topoisomerase II.- Topoisomerase II N-Terminal ATPase Clamp Stabilization.- Yeast Tools for Studying Type II Topoisomerases in Budding Yeast.- Use of Xenopus Egg Extracts to Study the Effects of Topoisomerase Poisons During Vertebrate DNA Replication.- Inducing and monitoring liquid-liquid phase separation by type II topoisomerases.- Analyzing Topoisomerase II Cleavage Complexes Using Flow Cytometry.- Kinetoplast DNA Decatenation Assay with Eukaryotic Topoisomerase II.- Recombinant Topoisomerase 2 Production Using Culture Human Cells.- Using PSICalc to Identify Protein Sequence Interdependencies.
