Description
Book SynopsisThis detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.
Table of ContentsPart I: Introduction to Blood-Brain Barrier (BBB) Physiology
1. Cells of the Blood-Brain Barrier: An Overview of the Neurovascular Unit in Health and Disease
Heather L. McConnell and Anusha Mishra
2. In Vitro Models of the Blood-Brain Barrier
Snehal Raut, Aditya Bhalerao, Behnam Noorani, and Luca Cucullo
Part II: Using Pluripotent Stem Cells in Models of the BBB
3. In Vitro Models of the Human Blood-Brain Barrier Based on Human Induced Pluripotent Stem Cells: Opportunities and Challenges
Iqra Pervaiz and Abraham J. Al-Ahmad
4. Induced Pluripotent Stem Cell (iPSC)-Derived Endothelial Cells to Study Bacterial–Brain Endothelial Cell Interactions
Eric R. Espinal, S. Jerod Sharp, and Brandon J. Kim
5. An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier
Mary Goodwin-Trotman, Krushangi Patel, and Alessandra Granata
6. A Three-Dimensional Brain-on-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes
Lumei Liu, Youngmi Koo, Teal Russell, and Yeoheung Yun
Part III: Co-Culture, Permeability, and Transwell Models of the BBB: Methods for Studying Shear Stress, Barrier Integrity and Breakdown
7. An Improved In Vitro Porcine Blood-Brain Barrier Model for Permeability Screening and Functional Studies
Adjanie Patabendige
8. Establishment of an In Vitro Model of Human Blood-Brain Barrier to Study the Impact of Ischaemic Injury
Rais Reskiawan A. Kadir, Mansour Alwjwaj, and Ulvi Bayraktutan
9. A Novel Dynamic Human In Vitro Model for Studying the Blood-Brain Barrier
Patricia Miranda-Azpiazu and Sikha Saha
10. A Dynamic, In Vitro BBB Model to Study the Effects of Varying Levels of Shear Stress
Gemma Molins Gutierrez, Jordi Martorell, Antonio G. Salazar-Martin, and Mercedes Balcells
Part IV: Microfluidic and Chip Models of the BBB
11. Novel, Emerging Chip Models of the BBB and Future Directions
Paul M. Holloway
12. Integrating Primary Astrocytes in a Microfluidic Model of the Blood-Brain Barrier
Eliana Lauranzano, Marco Rasile, and Michela Matteoli
13. Fabrication of Microtube-Embedded Chip to Mimic Blood-Brain Barrier Capillary Vessels
Dilshan Sooriyaarachchi, Shahrima Maharubin, and George Z. Tan
14. BBB-on-a-Chip: Modeling Functional Human BBB by Mimicking 3D Brain Angiogenesis Using Microfluidic Chip
Somin Lee, Minhwan Chung, and Noo Li Jeon
Part V: Models to Study Specific Pathologies at the BBB: Cancer, Neurodegeneration, and More
15. Cell Interplay Model to Assess the Impact of Glioma Cells on Blood-Brain Barrier Permeability
Cláudia Martins and Bruno Sarmento
16. An In Vitro Human Blood-Brain Barrier Model to Study Breast Cancer Brain Metastasis
Caroline Mysiorek, Lucie Dehouck, Fabien Gosselet, and Marie-Pierre Dehouck
17. An In Vivo Mouse Model to Study Blood-Brain Barrier Destabilization in the Chronic Phase of Stroke
Svetlana M. Stamatovic, Chelsea M. Phillips, Richard F. Keep, and Anuska V. Andjelkovic
18. Immunohistochemical Analysis of Tight Junction Proteins
Chris Greene and Matthew Campbell
19. An In Vitro BBB Model to Study Firm Shear-Stress Resistant Leukocyte Adhesion to Human Brain Endothelial Cells
Camilla Cerutti and Ignacio A. Romero
20. An In Vitro Model of the Blood-Brain Barrier (BBB) to Study Alzheimer's Disease: The Role of β-Amyloid and Its Influence on PBMC Infiltration
Simona Federica Spampinato, Yukio Takeshita, and Birgit Obermeier
