Description
Book SynopsisBioorthogonal Labeling and Click-Chemistry-Based Visualization of the
Tannerella forsythia Cell Wall.- Probing Membrane-Associated Cytoskeletal Oligomers of the Bacterial Divisome by Electron Microscopy and Tomography.- Visualization of a Cell Wall Hydrolase Inhibitor in
Fusobacterium nucleatum by Immunofluorescence Microscopy.- Computational and Biophysical Approaches to Identify Cell Wall-Associated Modulators in
Salmonella enterica Serovar Typhi.- Employing Cloning-Independent Mutagenesis of
Parvimonas micra for the Study of Cell Wall Biogenesis.- A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in
Fusobacterium nucleatum.- Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids.- Type I Lipoteichoic Acid (LTA) Detection by Western Blot.- Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spect
Table of Contents1. Bioorthogonal Labeling and Click-Chemistry-Based Visualization of the Tannerella forsythia Cell Wall
Stephen N. Hyland, Sreedevi Chinthamani, Sushanta Ratna, Kimberly A. Wodzanowski, Liam-Michael D. Sandles, Kiyonobu Honma, Catherine Leimkuhler-Grimes, and Ashu Sharma
2. Probing Membrane-Associated Cytoskeletal Oligomers of the Bacterial Divisome by Electron Microscopy and Tomography
Bo Hu and William Margolin
3. Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy
Kevin To, Timmie Britton, and Hung Ton-That
4. Computational and Biophysical Approaches to Identify Cell Wall-Associated Modulators in Salmonella enterica Serovar Typhi
Mukesh Kumar, Md. Anzarul Haque, and Punit Kaur
5. Employing Cloning-Independent Mutagenesis of Parvimonas micra for the Study of Cell Wall Biogenesis
Dustin L. Higashi, Zhengzhong Zou, Hua Qin, Jens Kreth, and Justin Merritt
6. A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum
Bibek G.C., Peng Zhou, and Chenggang Wu
7. Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids
Chen Zhang and Suliana Manley
8. Type I Lipoteichoic Acid (LTA) Detection by Western Blot
Charlotte Millership and Angelika Gründling
9. Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy
Jeanine Rismondo and Angelika Gründling
10. Single-Copy Gene Editing of a Cell Wall-Anchored Pilin in Actinomyces oris
Aadil H. Bhat, Asis Das, and Hung Ton-That
11. Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography
Nicole Cheung, Mabel Song, Christopher K. Sue, and Robert T. Clubb
12. Detection of Cell Wall-Anchoring Machinery by Immunogold-Labeling Thin-Section Electron Microscopy
Chungyu Chang and Hung Ton-That
13. Localization of the Remnant of a Cell Wall Sorting Signal and Its Interaction with a Sensor Kinase
Jeffrey W. Hall, Bruno P. Lima, Karen F. Johnstone, and Mark C. Herzberg
14. Determination of the Crystal Structure of the Cell Wall-Anchored Proteins and Pilins
Shivangi Tyagi, Rajnesh Kumari Yadav, and Vengadesan Krishnan
15. Tracking Cell Wall Anchored Proteins in Gram-Positive Bacteria
Salvatore J. Scaffidi and Wenqi Yu
16. Probing Bacterial Cell Division and Cell Envelope Biogenesis with Live-Cell Fluorescence Microscopy
Lauren R. Hammond, Maria L. White, and Prahathees J. Eswara
17. Assembling the Bacillus subtilis Spore Coat Basement Layer on Spherical Supported Lipid Bilayers
Taylor B. Updegrove, Domenico D’Atri, and Kumaran Ramamurthi
18. Structural Determination of Glucosyltransferase C by Cryo-Electron Microscopy
Qing Xie, Jianhao Cao, Hua Zhang, and Hui Wu
