Description

Book Synopsis

Imaging protocol for super-resolution microscopy in fixed T cells.- Visualization and quantification of NFAT1 translocation in T cells.- Deep Learning-Based Image Restoration and Super-Resolution for Fluorescence Microscopy: Overview and Resources.- Analysing Point Accumulation in Nanoscale Topography (PAINT) data to decipher T cell receptor cluster signalling.- Measuring NAADP-evoked Ca2+ release in permeabilized T cells.- An improved two-dimensional HPLC method for endogenous 2'-deoxy-ADPR and 2'-deoxy-NAD.- Analysis of Store-Operated Ca2+ Entry in primary T cells.- Monitoring Dynamic of cAMP Signaling in Primary Murine CD4+ T Cells via Live-Cell Imaging.- Using specific reporter mice to assess CD4+ T cell transdifferentiation.- Generation of antigen-specific CD4+ primary rat T cell lines.- Induction and assessment of activation in rat effector CD4+ memory Th cells.- Imaging BiTE-mediated activation of primary human CD8+ T cells.- Flow cytometric analysis of mouse brain CD8+ T cel

TCell Activation

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    A Hardback by Björn-Philipp Diercks

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      Book details

      Publisher Humana
      Published 5 April 2025
      ISBN-13 9781071644133
      978-1071644133
      ISBN-10 1071644130

      Description

      Book Synopsis

      Imaging protocol for super-resolution microscopy in fixed T cells.- Visualization and quantification of NFAT1 translocation in T cells.- Deep Learning-Based Image Restoration and Super-Resolution for Fluorescence Microscopy: Overview and Resources.- Analysing Point Accumulation in Nanoscale Topography (PAINT) data to decipher T cell receptor cluster signalling.- Measuring NAADP-evoked Ca2+ release in permeabilized T cells.- An improved two-dimensional HPLC method for endogenous 2'-deoxy-ADPR and 2'-deoxy-NAD.- Analysis of Store-Operated Ca2+ Entry in primary T cells.- Monitoring Dynamic of cAMP Signaling in Primary Murine CD4+ T Cells via Live-Cell Imaging.- Using specific reporter mice to assess CD4+ T cell transdifferentiation.- Generation of antigen-specific CD4+ primary rat T cell lines.- Induction and assessment of activation in rat effector CD4+ memory Th cells.- Imaging BiTE-mediated activation of primary human CD8+ T cells.- Flow cytometric analysis of mouse brain CD8+ T cel

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