Description
Book SynopsisThis volume describes the complete process of analyzing protein sequences by mass spectrometry including sample preparation, analysis, and data interpretation at a practical level. It provides sufficient experimental detail to carry out the experiments and consistently achieve good results.
Trade Review"[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods..." (SciTech Book News Vol. 25, No. 2 June 2001)
"...an informative, well-written and well-illustrated book with a large number of useful references.... It should prove invaluable to anyone working in the field of protein sequencing." (Spectroscopyeurope.com)
Table of ContentsAn Introduction to Protein Sequencing Using Tandem Mass Spectrometry.
The Primary Structure of Proteins and a Historical Overview of Protein Sequencing.
Fundamental Mass Spectrometry.
Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra.
Basic Polyacrylamide Gel Electrophoresis.
The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments.
Mass Spectrometric Analysis of Tryptic Digests.
Protein Identification by Database Searching.
Sequence Analysis of Novel Proteins.
The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry.
Index.
