Description

Book Synopsis

The SUMO Pathway.- SUMO Antibody Validation.- Native and Endogenous SUMO Site Identification Using Mass Spectrometry (NESSI-MS).- A Fluorometric Assay to Compare SUMO Isoforms Preferences in the SENP/ULP Family.- Production and Purification of SUMO-UBC9 and SUMO-RANGAP1CTD.- Synthesizing Defined Ubiquitin-Modified SUMO Dimers.- In Vitro SUMOylation Assays with a DNA-Sensitive Multi-Subunit E3 Ligase.- Cell-Based Assays to Study SUMOylation in Drosophila.- SUMO-Activated Target Traps (SATTs) for the Identification of Specific SUMOylated Proteins for an E3 Ligase of Interest.- Synthesis and Application of SUMO-Based Probes for SUMO Protease Profiling.- Measurement of Substrate SUMOylation in Real Time by Fluorescence Spectroscopy.- Production and Purification of Recombinant SUMOylation Enzymes and SUMO Proteins.- Non-Hydrolysable ADP-Ribosylated SUMO2 via Copper(I) Catalyzed Azide Alkyne Cycloaddition.- A Vivo-Morpholino-Based Approach to Block Paralog-Specific SUMOylation.- Bimolecular Fluorescence Complementation Assay to Evaluate the Interaction between SUMO and Its Target Proteins in Cells.

SUMO

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    A Hardback by Emilio Lecona

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      Publisher: Humana
      Publication Date: Publication Date: 12/08/2025
      ISBN13: 9781071647097, 978-1071647097
      ISBN10:
      Also in:
      Cellular biology (cytology)

      Description

      Book Synopsis

      The SUMO Pathway.- SUMO Antibody Validation.- Native and Endogenous SUMO Site Identification Using Mass Spectrometry (NESSI-MS).- A Fluorometric Assay to Compare SUMO Isoforms Preferences in the SENP/ULP Family.- Production and Purification of SUMO-UBC9 and SUMO-RANGAP1CTD.- Synthesizing Defined Ubiquitin-Modified SUMO Dimers.- In Vitro SUMOylation Assays with a DNA-Sensitive Multi-Subunit E3 Ligase.- Cell-Based Assays to Study SUMOylation in Drosophila.- SUMO-Activated Target Traps (SATTs) for the Identification of Specific SUMOylated Proteins for an E3 Ligase of Interest.- Synthesis and Application of SUMO-Based Probes for SUMO Protease Profiling.- Measurement of Substrate SUMOylation in Real Time by Fluorescence Spectroscopy.- Production and Purification of Recombinant SUMOylation Enzymes and SUMO Proteins.- Non-Hydrolysable ADP-Ribosylated SUMO2 via Copper(I) Catalyzed Azide Alkyne Cycloaddition.- A Vivo-Morpholino-Based Approach to Block Paralog-Specific SUMOylation.- Bimolecular Fluorescence Complementation Assay to Evaluate the Interaction between SUMO and Its Target Proteins in Cells.

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