Description
Book SynopsisThis volume presents the most useful laboratory protocols in strigolactones(SL) research. Chapters guide readers through wet-lab paths, issues around stability, protocols to evaluate SL activity, effects towards soil inhabitants such as parasitic plants, mycorrhizal and non-mycorrhizal fungi, nodulating bacteria, and protocols to assess effects on plant development are discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Strigolactones: Methods and Protocols aims to deliver a clear-cut and standardized set of experimental protocols to a broad scientific community.
Table of Contents
Part I STRIGOLACTONE CHEMISTRY
1. Evaluation and Quantification of Natural Strigolactones from Root Exudates
Xiaonan Xie, Kaori Yoneyama, and Takahito Nomura Koichi Yoneyama
2. Isolation and Identification of Naturally Occurring Strigolactones
Kotomi Ueno, Takatoshi Wakabayashi, and Yukihiro Sugimoto
3. Chemical Synthesis of Triazole-Derived Suppressors of Strigolactone Functions
Shisanku Ito, Ko Kikuzato, Hidemitsu Nakamura, and Tadao Asami
4. Synthesis of Simple Strigolactone Mimics
Tomáš Pospíšil
5. Synthesis of Strigolctones Analogues and Evaluation of their Stability in Solution
Daniel Blanco-Ania and Binne Zwanenburg
Part II: STRIGOLACTONES and SOIL BIOTA
6. Strigolactone-like Bioactivity Via Parasitic Plant Germination Bioassay
Jean-Bernard Pouvreau, Lucie Poulin, Sarah Huet, and Philippe Delavault
7. Evaluation of the Effect of Strigolactones and Synthetic Analogues On Fungi
Valentina Fiorilli, Mara Novero, and Luisa Lanfranco
8. Analysing the Effect of Strigolactones on the Motility Behaviour of Rhizobia
Lydia M. Bernabéu-Roda, Juan Antonio López-Ráez, and María J. Soto
9. Chemotropic Assay for Testing Fungal Response to Strigolactones and Strigolactone-Like Compounds
Rocío Pineda-Martos, Antonio Di Pietro, and Davide Turrà
Part III: STRIGOLACTONES AS PLANT HORMONES
10. Methods for Phenotyping Shoot Branching and Testing Strigolactone Bioactivity for Shoot Branching in Arabidopsis and Pea
Aitor Muñoz, Jean-Paul Pillot, Pilar Cubas, and Catherine Rameau
11. Bioassays for the Effects of Strigolactones and Other Small Molecules on Root and Root Hair Development
José Antonio Villaécija-Aguilar, Sylwia Struk, Sofie Goormachtig, and Caroline Gutjahr
12. Methods for Medium-Scale Study of the Biological Effects of Strigolactone-Like Molecules on the Moss Physcomitrella patens
Ambre Guillory and Sandrine Bonhomme
13. Controlled Assays for Phenotyping the Effects of Strigolactone-like Molecules on Arbuscular Mycorrhiza Development
Salar Torabi, Kartikye Varshney, José A. Villaécija-Aguilar, Andreas Keymer, and Caroline Gutjahr
14. Application of Strigolactones to Plant Roots to Influence the Formation of Symbioses
Eloise Foo
Part IV: BIOACTIVITY ASSAYS
15.Evaluation of the Bioactivity Oof Strigolactone-Related Molecules by a Quantitative Luminometer Bioassay
Elena Sánchez, Pilar Cubas, Francesca Cardinale, and Ivan Visentin
16. A Protoplast-based Bioassay to Quantify Strigolactone Activity in Arabidopsis using StrigoQuant
Justine Braguy, Sophia L. Samodelov, Jennifer Andres, Rocio Ochoa-Fernandez, Salim Al Babili, and Matias D. Zurbriggen
17. Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies
Alexandre de Saint Germain, Guillaume Clavé, and François-Didier Boyer
18. The use of Differential Scanning Fluorimetry to Assess Strigolactone Receptor Function
Cyril Hamiaux, Bart J Janssen, and Kimberley Snowden
19. Structural Analysis of Strigolactone-Related Gene Products
Gunilla H. Carlsson, Dirk Hasse, and Inger Andersson
