Description
Book SynopsisSpin-label electron paramagnetic resonance (EPR) spectroscopy is a versatile molecular probe method that finds wide application in molecular biophysics and structural biology. This book provides the first comprehensive summary of basic principles, spectroscopic properties, and use for studying biological membranes, protein folding, supramolecular structure, lipid-protein interactions, and dynamics. The contents begin with discussion of fundamental theory and practice, including static spectral parameters and conventional continuous-wave (CW) spectroscopy. The development then progresses, via nonlinear CW-EPR for slower motions, to the more demanding time-resolved pulse EPR, and includes an in-depth treatment of spin relaxation and spectral line shapes. Once the spectroscopic fundamentals are established, the final chapters acquire a more applied character. Extensive appendices at the end of the book provide detailed summaries of key concepts in magnetic resonance and chemical physic
Table of Contents
1. Introduction. 2. The Nitroxide EPR Spectrum. 3. Hyperfine Interactions and g-Vaules. 4. Polarity Dependance. 5. Spin Relaxation Theory. 6. EPR Lineshape Theory. 7. Dynamics and Rotational Diffusion. 8. Dynamics and Orientational Ordering (Liquid Crystals and Membranes). 9. Spin-Spin Interactions. 10. Spin-Latice Relaxation. 11. Nonlinear and Saturation-Transfer EPR. 12. Saturation-Recovery EPR and ELDOR. 13 Spin-Echo EPR. 14. ESEEM and ENDOR: Hyperfine Spectroscopy. 15. Distance Measurements. 16. Site-Directed Spin Labelling (SDSL)
