Description
Book SynopsisSmall-angle scattering of X-rays (SAXS) and neutrons (SANS) is an established method for the structural characterization of biological objects in a broad size range from individual macromolecules (proteins, nucleic acids, lipids) to large macromolecular complexes. SAXS/SANS is complementary to the high resolution methods of X-ray crystallography and nuclear magnetic resonance, allowing for hybrid modeling and also accounting for available biophysical and biochemical data. Quantitative characterization of flexible macromolecular systems and mixtures has recently become possible. SAXS/SANS measurements can be easily performed in different conditions by adding ligands or binding partners, and by changing physical and/or chemical characteristics of the solvent to provide information on the structural responses. The technique provides kinetic information about processes like folding and assembly and also allows one to analyze macromolecular interactions. The major factors promoting the incr
Trade ReviewThis book is a masterly treatment by four authors with many decades of experience of SAXS and SANS and who are from world class synchrotron X-ray and neutron research facilities. From the basics and on through the instrumentation and data analysis descriptions, as well as numerous research themes and examples, this book will be invaluable to newcomers and be useful to the specialists. It also should be read in detail by structural biology researchers whether principally from crystallography, bio NMR, EM or bioinformatics. It's surely going to be a classic book. * John Helliwell, University of Manchester *
Small angle X-ray and neutron scattering from solutions of biological macromolecules is an essential book covering the theory, experiment, data analysis, and applications of small-angle scattering (SAS) ... No laboratory interested in SAS should be without a copy and it provides a good background to the capabilities, limitations, and practical application of the technique. The authors should be commended and the book is strongly recommended. * Crystallography Reviews *
Table of ContentsIntroduction ; PART I. THEORY AND EXPERIMENT ; 1. Basics of small-angle scattering ; 2. X-ray and neutron scattering instruments ; 3. Experimental practice and data processing ; PART II. DATA ANALYSIS METHODS ; 4. Monodisperse systems ; 5. Polydisperse and interacting systems ; PART III. BIOLOGICAL APPLICATIONS OF SOLUTION SAS ; 6. Static structral studies ; 7. Kinetic and perturbation studies ; 8. Analysis of interparticle interactions ; 9. SAS in multidisciplinary studies ; Conclusions and future outlook ; Appendix 1: Waves, complex number, Fourier transforms, convolution, correlation ; Appendix 2: Spherical harmonics formalism ; Appendix 3: Interactions between spherical molecules ; Appendix 4: List of Web resources
