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Book Synopsis

Guidelines for RNA Analysis by Reverse Transcription Quantitative Polymerase Chain Reaction.- Rare Transcripts Quantification by Strand Specific RT-qPCR.- Detection of Cancer Associated Gene Fusion by Ligation Dependent RT-PCR.- Isolation of a Specific Transcript Using Biotinylated Antisense Probes.- Transcriptome-Wide Characterization of Protein:RNA Interactions with UV-Crosslinking Immunoprecipitation and Sequencing.- Targeted 16S rRNA Gene Sequencing for Water Samples.- RNA-Based High-Throughput Sequencing of the Human Immunoglobulin Repertoire.- 5’RACE Method for RNA-Based Sequencing of the Mouse Immunoglobulin Repertoire.- A Wet Lab Procedure for Gene Fusion Analysis by Targeted RNA Next Generation Sequencing in Lung Cancer.- Simultaneous Profiling of Transcripts and Genomic Regions Associated with Nuclear Bodies using the RNA-DNA High-Salt Recovered Sequence (RD-HRS) Method.- Functional Evaluation of Splice Variants Using a Minigene Strategy.- Design and Evaluation of a Truncated Protein Induction Strategy by Antisense Oligonucleotide-Mediated Exon Skipping.- Modifying 3'-End RNA Isoforms using Antisense Oligonucleotides.- A Mass Spectrometry-Based Pipeline for Analyzing RNA Modifications in Cells and Tissues.- Inhibition of Nonsense-Mediated Decay in Dorsal Root Ganglion Neurons.- Knockdown of Gene Expression Following Activation of the NMD Pathway Using Splice-Switching Oligonucleotides.- Screening Methods for NMD Inhibitors or Readthrough Activators.

RNA Analysis

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    A Hardback by Laurent Delpy

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      Book details

      Publisher Humana
      Published 25 August 2025
      ISBN-13 9781071647257
      978-1071647257
      ISBN-10 1071647253
      Also in:
      Genetics (non-medical)

      Description

      Book Synopsis

      Guidelines for RNA Analysis by Reverse Transcription Quantitative Polymerase Chain Reaction.- Rare Transcripts Quantification by Strand Specific RT-qPCR.- Detection of Cancer Associated Gene Fusion by Ligation Dependent RT-PCR.- Isolation of a Specific Transcript Using Biotinylated Antisense Probes.- Transcriptome-Wide Characterization of Protein:RNA Interactions with UV-Crosslinking Immunoprecipitation and Sequencing.- Targeted 16S rRNA Gene Sequencing for Water Samples.- RNA-Based High-Throughput Sequencing of the Human Immunoglobulin Repertoire.- 5’RACE Method for RNA-Based Sequencing of the Mouse Immunoglobulin Repertoire.- A Wet Lab Procedure for Gene Fusion Analysis by Targeted RNA Next Generation Sequencing in Lung Cancer.- Simultaneous Profiling of Transcripts and Genomic Regions Associated with Nuclear Bodies using the RNA-DNA High-Salt Recovered Sequence (RD-HRS) Method.- Functional Evaluation of Splice Variants Using a Minigene Strategy.- Design and Evaluation of a Truncated Protein Induction Strategy by Antisense Oligonucleotide-Mediated Exon Skipping.- Modifying 3'-End RNA Isoforms using Antisense Oligonucleotides.- A Mass Spectrometry-Based Pipeline for Analyzing RNA Modifications in Cells and Tissues.- Inhibition of Nonsense-Mediated Decay in Dorsal Root Ganglion Neurons.- Knockdown of Gene Expression Following Activation of the NMD Pathway Using Splice-Switching Oligonucleotides.- Screening Methods for NMD Inhibitors or Readthrough Activators.

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