Description
Book SynopsisThis volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one or
Table of Contents1. Investigating Neurogenesis in Birds
Tania Rodrigues, Laurent Brodier, and Jean-Marc Matter
2. Studying in vivo Retinal Progenitor Cell Proliferation in Xenopus laevis
Cindy X. Kha, Dylan J. Guerin, and Kelly Ai-Sun Tseng
3. Three-dimensional Culture of Mouse Eyecups
Raven Diacou, Punita Bhansali, and Wei Liu
4. Live Imaging of Mouse Retinal Slices
Anthony P. Barrasso and Ross A. Poché
5. Retinal Strip Culture for Studying Ganglion Cell Axon Growth
Masayuki Yamashita
6. Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants
Chuan-Chin Chiao, Chin-I Lin, and Meng-Jung Lee
7. Adeno-associate Virus as Gene Delivery Vehicle into Retina
Shuyun Deng and Kazuhiro Oka
8. Transposon-mediated Stable Suppression of Gene Expression in the Developing Chick Retina
Masaru Nakamoto and Chizu Nakamoto
9. A Simple Guide for Generating BAC Transgenic Animals for Retinal Research
Cavit Agca and Christian Grimm
10. Identification and Characterization of Cis-regulatory Elements for Photoreceptor Type-specific Transcription in Zebrafish
Wei Fang, Yi Wen, and Xiangyun Wei
11. Ultrasensitive RNAscope In Situ Hybridization System on Embryonic and Adult Mouse Retinas
Takae Kiyama and Chai-An Mao
12. Single Cell Capture, RNA-seq and Transcriptome Analysis from the Neural Retina
Rachayata Dharmat, Sangbae Kim, Yumei Li, and Rui Chen
13. Genetically Directed Sparse Labeling System for Anatomical Studies of Retinal Ganglion Cells
Leila Jamal, Takae Kiyama, and Chai-An Mao
14. Intracellular Dye Microinjection in Retinal Morphological and Communication Studies
Ye Long
15. Multi-electrode Array Recording of Mouse Retinas Transplanted with Stem-cell Derived Retinal Sheets
Hung-Ya Tu and Take Matsuyama
16. Paired Recording to Study Electrical Coupling between Photoreceptors in Mouse Retina
Nange Jin, Zhijing Zhang, Kimberly A. Mankiewicz, and Christophe P. Ribelayga
