Description

Book Synopsis

The book fills the void between rigorous theoretical discussions of ligand binding for physical chemists (often beyond the needs of bench experimentalists in the life sciences) and compilations of experimental recipes that offer scant regard for the appropriateness of the protocol for the particular ligand interaction under investigation.

Table of Contents

Introduction: The Analysis of Simple Binding Responses.

Experimental Methods Based on Phase Separation.

Spectral Studies of Ligand Binding.

Other Direct Procedures for Characterizing Ligand Binding.

Binding Constants from Competitive Binding Assays.

Sigmoidal Binding Curves: Analysis and Interpretation.

Complicating Factors in the Analysis of Binding Curves.

Studies of DNA-Ligand Interactions.

Concluding Remarks.

Glossary of Symbols.

References.

Index.

Quantitative Characterization of Ligand Binding

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    A Paperback by Donald J. Winzor, William H. Sawyer

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      Book details

      Publisher Wiley-Liss Inc.,U.S.
      Published 16 November 1995
      ISBN-13 9780471059585
      978-0471059585
      ISBN-10 0471059587
      Also in:
      Biochemistry Chemistry

      Description

      Book Synopsis

      The book fills the void between rigorous theoretical discussions of ligand binding for physical chemists (often beyond the needs of bench experimentalists in the life sciences) and compilations of experimental recipes that offer scant regard for the appropriateness of the protocol for the particular ligand interaction under investigation.

      Table of Contents

      Introduction: The Analysis of Simple Binding Responses.

      Experimental Methods Based on Phase Separation.

      Spectral Studies of Ligand Binding.

      Other Direct Procedures for Characterizing Ligand Binding.

      Binding Constants from Competitive Binding Assays.

      Sigmoidal Binding Curves: Analysis and Interpretation.

      Complicating Factors in the Analysis of Binding Curves.

      Studies of DNA-Ligand Interactions.

      Concluding Remarks.

      Glossary of Symbols.

      References.

      Index.

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