Description

Book Synopsis
The book fills the void between rigorous theoretical discussions of ligand binding for physical chemists (often beyond the needs of bench experimentalists in the life sciences) and compilations of experimental recipes that offer scant regard for the appropriateness of the protocol for the particular ligand interaction under investigation.

Table of Contents
Introduction: The Analysis of Simple Binding Responses.

Experimental Methods Based on Phase Separation.

Spectral Studies of Ligand Binding.

Other Direct Procedures for Characterizing Ligand Binding.

Binding Constants from Competitive Binding Assays.

Sigmoidal Binding Curves: Analysis and Interpretation.

Complicating Factors in the Analysis of Binding Curves.

Studies of DNA-Ligand Interactions.

Concluding Remarks.

Glossary of Symbols.

References.

Index.

Quantitative Characterization of Ligand Binding

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    A Paperback / softback by Donald J. Winzor, William H. Sawyer

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      View other formats and editions of Quantitative Characterization of Ligand Binding by Donald J. Winzor

      Publisher: John Wiley & Sons Inc
      Publication Date: 16/11/1995
      ISBN13: 9780471059585, 978-0471059585
      ISBN10: 0471059587
      Also in:
      Biochemistry Chemistry

      Description

      Book Synopsis
      The book fills the void between rigorous theoretical discussions of ligand binding for physical chemists (often beyond the needs of bench experimentalists in the life sciences) and compilations of experimental recipes that offer scant regard for the appropriateness of the protocol for the particular ligand interaction under investigation.

      Table of Contents
      Introduction: The Analysis of Simple Binding Responses.

      Experimental Methods Based on Phase Separation.

      Spectral Studies of Ligand Binding.

      Other Direct Procedures for Characterizing Ligand Binding.

      Binding Constants from Competitive Binding Assays.

      Sigmoidal Binding Curves: Analysis and Interpretation.

      Complicating Factors in the Analysis of Binding Curves.

      Studies of DNA-Ligand Interactions.

      Concluding Remarks.

      Glossary of Symbols.

      References.

      Index.

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