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Cross-Species Proximity-Dependent Protein Biotinylation: A Standardized Approach for Mapping Proxeomes.- BioID in Bacteria: Selection of a Suitable Biotin Ligase for Proxeome Mapping.- Uncovering Transient Protein Interactions in the Yersinia enterocolitica Type III Secretion System Using MiniTurbo-Mediated Proximity Labeling.- Exploring Bacterial Surface Proteome Dynamics During Infection Using Proximity Labeling.- Identification of In Vivo Protein Networks Using MiniTurbo Proximity Labeling in Bacteria.- Unraveling N-Terminal Proteoform Interactomes via Multiplexed Recombineering in Salmonella.- Probing Pairwise Protein-Protein Interactions with the Bacterial POLAR Assay.- Detection and Quantification of Biotinylated Proteins for TurboID-Based Proximity Labeling Mass Spectrometry in Arabidopsis.- Detection and Quantification of Biotinylated Sites for TurboID-Based Proximity Labeling Mass Spectrometry in Arabidopsis.- Coupling Affinity Purification with Proximity Labeling in Arabidopsis: The APEAL Approach.- Characterization of the Protein Interactome of Membrane-Bound Transcription Factors Using TurboID-Based Proximity Labeling In Planta.- Identification of Cytosol-Facing Organelle Membrane-Proximal Proteins via Proximity-Dependent Biotinylation.- BioID2-Based Proximity Labeling in Adult Zebrafish.- On-Demand Proximity Labeling Using Light-Activated BioID.- Tissue-Specific Biotinylation for Interorgan Communication.- Spatiotemporally-Resolved Profiling of Protein Movement by TransitID.- An Integrated High-Throughput Proteocistromic Framework for Mapping the Interactome and Targetome of Human Transcription Factors.- Step-by-Step Application of Proximity-Dependent Biotinylation Tools for Identification of Astrocyte and Neuron Subproteomes In Vivo Across the Whole Brain.- Antibody-Mediated Protein A-APEX2 Labeling (AMAPEX) for Proximity Proteome Exploration.- A Ready-to-Use Data Analysis Pipeline for BioID Experiments Using Data-Dependent or Data-Independent Acquisition Mass Spectrometry.

ProximityDependent Protein Biotinylation

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    A Hardback by Petra Van Damme

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      Book details

      Publisher Humana
      Published 13 August 2025
      ISBN-13 9781071646939
      978-1071646939
      ISBN-10 1071646931

      Description

      Book Synopsis

      Cross-Species Proximity-Dependent Protein Biotinylation: A Standardized Approach for Mapping Proxeomes.- BioID in Bacteria: Selection of a Suitable Biotin Ligase for Proxeome Mapping.- Uncovering Transient Protein Interactions in the Yersinia enterocolitica Type III Secretion System Using MiniTurbo-Mediated Proximity Labeling.- Exploring Bacterial Surface Proteome Dynamics During Infection Using Proximity Labeling.- Identification of In Vivo Protein Networks Using MiniTurbo Proximity Labeling in Bacteria.- Unraveling N-Terminal Proteoform Interactomes via Multiplexed Recombineering in Salmonella.- Probing Pairwise Protein-Protein Interactions with the Bacterial POLAR Assay.- Detection and Quantification of Biotinylated Proteins for TurboID-Based Proximity Labeling Mass Spectrometry in Arabidopsis.- Detection and Quantification of Biotinylated Sites for TurboID-Based Proximity Labeling Mass Spectrometry in Arabidopsis.- Coupling Affinity Purification with Proximity Labeling in Arabidopsis: The APEAL Approach.- Characterization of the Protein Interactome of Membrane-Bound Transcription Factors Using TurboID-Based Proximity Labeling In Planta.- Identification of Cytosol-Facing Organelle Membrane-Proximal Proteins via Proximity-Dependent Biotinylation.- BioID2-Based Proximity Labeling in Adult Zebrafish.- On-Demand Proximity Labeling Using Light-Activated BioID.- Tissue-Specific Biotinylation for Interorgan Communication.- Spatiotemporally-Resolved Profiling of Protein Movement by TransitID.- An Integrated High-Throughput Proteocistromic Framework for Mapping the Interactome and Targetome of Human Transcription Factors.- Step-by-Step Application of Proximity-Dependent Biotinylation Tools for Identification of Astrocyte and Neuron Subproteomes In Vivo Across the Whole Brain.- Antibody-Mediated Protein A-APEX2 Labeling (AMAPEX) for Proximity Proteome Exploration.- A Ready-to-Use Data Analysis Pipeline for BioID Experiments Using Data-Dependent or Data-Independent Acquisition Mass Spectrometry.

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