Description
Book SynopsisThis detailed book explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields.Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.
Table of Contents1. Requirements and Preparedness for Attending a Viral Disease Outbreak in Pig Farms
Dipak Deka, Pankaj Kumar Dhaka, Ujjwal Kumar De, Obli Rajendran Vinodh Kumar, and Yashpal Singh Malik
2. Collection of Samples, Their Preservation, and Transportation
Ashok Kumar, Kaushal Kishor Rajak, Ajay Kumar Yadav, Vishal Rai, Mukesh Bhatt, R.P. Singh, and R.K. Singh
3. Methods for Quantification of Viruses
Mukesh Bhatt, Chris Einstein, Kiran, Arfa Fayaz, Vishal Rai, Monu Karki, Ashok Kumar, Ajay Kumar Yadav, and Kaushal Kishor Rajak
4. Protocols for Isolation of Genetic Materials from RNA Viruses
Nihar Nalini Mohanty, Vikas Gupta, Laxmi Narayan Sarangi, Rohini Bhat, and Sathish B. Shivachandra
5. Multiplex PCR for Diagnosis of Porcine Diseases
Manjisha Choudhury, Ajay Kumar Yadav, Seema Rani Pegu, Rajib Deb, and Swaraj Rajkhowa
6. Protocols for Isolation of Plasmid DNA
Vinod Kumar Singh, Vikas Gupta, and Chayanika Das
7. Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases
Rajib Deb, Ajay Kumar Yadav, Gyanendra Singh Sengar, Seema Rani Pegu, Souvik Paul, Swaraj Rajkhowa, and Vivek Kumar Gupta
8. RNA-PAGE-Based Diagnosis of Viral Diseases
Naveen Kumar, Geetika Kaur, Shubhankar Sircar, Zunjar Dubbal, R.S. Sethi, and Yashpal Singh Malik
9. Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases
Vinay G. Joshi, Anu Kumari, Sushila Maan, Tarun Kumar, and Satish Kumar
10. Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases
Ajay Kumar Singh, Soumen Naskar, Pramod W. Ramteke, and Rohit Kumar
11. Aptamers as Diagnostic Markers for Viral Infections of Veterinary Importance
Victoria C. Khangembam and Dimpal Thakuria
12. Antibody-Based Sensors for Pathogen Detection
Nirmita Dutta, Akhil Kumar, Anu Kumari, Sushila Maan, Gorachand Dutta, and Vinay G. Joshi
13. Lateral Flow Assay for Diagnosis of Pig Viral Diseases
Aditya Prasad Sahoo and Rajib Deb
14. Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases
Yoya Vashi and Sachin Kumar
15. Protocols for Immunofluorescence Techniques
Deepika Bisht, Shikha Saxena, Nitish Singh Kharayat, and Siddharth Gautam
16. Polymerase Spiral Reaction (PSR) for the Diagnosis of Porcine Viral Diseases
Vikas Gupta, Nihar Nalini Mohanty, and Vinod Kumar Singh
17. Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases
Yoya Vashi and Sachin Kumar
18. Cell Culture System for Porcine Virus Isolation and Propagation
Vishal Rai, Kaushal Kishor Rajak, Kiran, Arfa Fayaz, Monu Karki, Chris Einstein, Mukesh Bhatt, Ashok Kumar, and Ajay Kumar Yadav
19. An Overview of Mouse Monoclonal Antibody Production
Kaushal Kishor Rajak, Kiran, Arfa Fayaz, Vishal Rai, Monu Karki, Chris Einstein, Mukesh Bhatt, Ashok Kumar, Ajay Kumar Yadav, and R.P. Singh
20. Nucleic Acid Hybridization Techniques for Viral Disease Diagnosis: A Detailed Perspective
B.V. Sunil Kumar, Himalaya Bhardwaj, Ankita Gurao, Naveen Kumar, and Yashpal Singh Malik
21. Ligase Detection Reaction–Fluorescent Microsphere Assay
A. Raja
22. ELISA as a Diagnostic Weapon
Ramya Kalaivanan and Sankar Palanisamy
23. SDS-PAGE and Western Blotting: Basic Principles and Protocol
Mukesh Bhatt, Vishal Rai, Ashok Kumar, Kiran, Ajay Kumar Yadav, Kaushal Kishor Rajak, Vikas Gupta, Vishal Chander, and R.K. Avasthe
24. Immune Assays as Diagnostic for Pig Viral Diseases
Prabhakar Maurya, Jupi Talukdar, Sarmistha Debbarma, Monuj Kumar Doley, and Luit Barkalita
25. Production of Virus-Like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis
Hemanta Kumar Maity, Rajib Deb, Sinéad Lyons, and Ian M. Jones
26. Good Laboratory Practices and Biosafety Containments in a Virology Laboratory
Yashpal Singh Malik, Anuradha Sharma, Niraj Kumar Singh, B.T. Naveen Kumar, and Naveen Kumar
