Description
Book SynopsisThis book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.
Trade Review"This book is an impressive picture of the tremendous evolution of phycology during the ten past years. Each PhD student and postdoctoral researcher should have it in its laboratory; he will find in it the basic most recent protocols ready to use for his study, and the matter for imaginative, limitless experiments."
—Bruno de Reviers, Muséum National d’histoire Naturelle, Paris, France
""This book is a well written and informative guide to methods for macroalgal (seaweed) culture and analysis. It is notable for the broad coverage of macroalgal biology, from culture approaches, through methods for compositional analysis to the newer molecular approaches of metabolomics and transcriptomics. It will certainly prove to be a useful resource for those involved in macroalgal culture and biotechnology."—John Beardall, Monash University, Melbourne, Victoria, Australia
Protocols for Macroalgae Research describes state-of-the-art approaches and applications in seaweed research. The book is comprised of 31 protocols in all and provides critical information regarding the cultivation and preservation of seaweeds, their chemical composition, as well as their cellular and molecular characterization. This type of research is critical and timely, as the last few decades have witnessed remarkable advancements in phycological research worldwide. Consideration of algal resources for food, fuel, chemicals, feed, and fertilizer has furthered the development of diverse laboratory techniques that not only advance their utilization substantially but also provided new insights in understanding the molecular basis of their form and function. This recent progress has enabled researchers to determine and quantify various metabolites and organic macromolecules such as carbohydrates, proteins, and lipids.
Protocols for Macroalgae Research thus uses macroalgae as a model organism for addressing fundamental research problems. No recent comprehensive literature of this format exists on this topic. As such, this state-of-the-art protocol text both develops and standardizes the various macroalgae approaches located within the field. It does so by featuring a wide-range of protocols from invited experts; each protocol features a brief introductory overview, an inventory of materials and reagents, and step-by-step experimental procedures which include helpful notes offering care and cautions. This protocol book is an invaluable reference for beginning and preexisting researchers within the field of macroalgae.
"This book is an impressive picture of the tremendous evolution of phycology during the ten past years. Each PhD student and postdoctoral researcher should have it in its laboratory; he will find in it the basic most recent protocols ready to use for his study, and the matter for imaginative, limitless experiments."
—Bruno de Reviers, Muséum National d’histoire Naturelle, Paris, France
"There have been prior textbooks on methods in phycology, but this book brings techniques up to date. The book seeks to aid the reader to actually be able to replicate research protocols. An array of subject matter experts has been accumulated to produce 31chapters. The book is logically laid out with the reader in mind, explains techniques, and provides best-practice advice on how to carry out protocols successfully."
—Alan T Critchley, Cape Breton University, Nova Scotia, Canada
"This book is a well written and informative guide to methods for macroalgal (seaweed) culture and analysis. It is notable for the broad coverage of macroalgal biology, from culture approaches, through methods for compositional analysis to the newer molecular approaches of metabolomics and transcriptomics. It will certainly prove to be a useful resource for those involved in macroalgal culture and biotechnology."—John Beardall, Monash University, Melbourne, Victoria, Australia
"The book covers a wide range of topics: From large-scale macroalgal cultivation to the biochemical and molecular characterization. I can see that each of the individual chapters has been written by leading experts in the respective field. I am very happy about the approach to present theoretical background first and then provide step-by-step protocols to help implementing methods in lab routines This book represents a significant enrichment to the lab work of each phycology scholar. My PhD students will be very happy with it!"
—Kai Bischof, University of Bremen, Germany
"A well-balanced selection of topics covering all main subjects relating to 21th century phycology (genetics – development – aquaculture). This is what makes this book quite unique and given the interest in seaweed for aquaculture in the western part of the world, I think this book will be warmly received."—Olivier De Clerck, Ghent University, Belgium
"A really useful new addition to the literature on algae and their uses, with detailed methods for the study of these organisms, which have considerable potential in the blue economy. Thirty-one chapters in three sections (cultivating and preserving seaweeds: chemical composition; cellular and molecular characterization), by experts in their fields. Research oriented, with detailed explanations on how new techniques can be applied to the burgeoning new industries based on algal materials. Covers a wide variety of detailed methods, from culture (from commercial aquaculture to bacteria-free algal studies); through chemical constituents and health aspects, molecular techniques (including cloning and transformation); as well as detailed modern microscopic techniques on macroalgae and their related bacterial flora."—John J Bolton, University of Cape Town, South Africa
"A timely, well produced book containing state of the art information on a range of methodologies relevant to seaweed scientists. It fills a large gap in the available literature and will be an essential resource for both budding and experienced macroalgal researchers."—Catriona Hurd, University of Tasmania, Hobart, Australia
Table of ContentsCONTENTS
Section I: Cultivating and preserving seaweeds
Chapter 1 Seaweed in high-energy environments: Protocol
to move Saccharina cultivation offshore
Chapter 2 Cultivation protocol for Saccharina latissima
Chapter 3 Derivation of clonal stock cultures and hybridization
of kelps: A tool for strain preservation and breeding
programs
Chapter 4 Cryopreservation of macroalgae
Chapter 5 Unraveling seaweeds bacteriomes: From field site
to computer screen
Chapter 6 Heavy metal ecotoxicity on the early life history
stages of macroalgae
Chapter 7 A simple protocol for a rapid and consistent
production of a large number of viable protoplasts
from the Ulvophycean species
Chapter 8 Purification of sporulation and swarming inhibitors
from Ulva: Application in algal life-cycle controlling
Chapter 9 Preparation of axenic cultures in Ulva (Chlorophyta)
Section II: Chemical composition
Chapter 10 Biochar production from seaweeds
Chapter 11 Identification and quantification of laminarins
in brown algae
of macroalgae
Chapter 12 Determination of carbohydrate composition
Chapter 13 Quantification of proteins in seaweeds
Chapter 14 Comprehensive phytohormone quantification
in the red alga Pyropia yezoensis by liquid
chromatography–mass spectrometry
Chapter 15 Total phenolic content and antioxidant capacity
analysis of seaweed biomass
Chapter 16 Extraction of phycocyanin and phycoerythrin pigments
Chapter 17 Quantification and localization of reactive oxygen
species in marine macrophytes
Chapter 18 Metabolomics of intra- and extracellular metabolites
from micro- and macroalgae using GC–MS
and LC–MS
Chapter 19 Preparative extraction of exometabolites
from seaweed surfaces
Florian Weinberger
Chapter 20 Disruption-free solid-phase extraction of surface
metabolites from macroalgae
Emilio Cirri and Georg Pohnert
Section III: Cellular and molecular characterization
Chapter 21 The immunodetection and in situ imaging of
cell-wall polysaccharides in brown algae
Chapter 22 Atomic force microscopy based analysis of cell-wall
elasticity in macroalgae
Chapter 23 Dynamic and microscale mapping of cell growth:
Case of Ectocarpus filament cells
Chapter 24 Actin fluorescent staining in the filamentous brown
alga Ectocarpus siliculosus
Chapter 25 Cryofixation of brown algae for transmission
electron microscopy
Chapter 26 Probing the subcellular topography of
seaweeds: Transmission electron microscopy,
immunocytochemistry, and correlative light
microscopy
Chapter 27 Coralline algae preparation for scanning electron
microscopy and optical microscopy
Chapter 28 Extraction of high quality RNA from brown algae
for transcriptomic analysis
Chapter 29 Induction of sexual reproduction in Spirogyra
cultures for laser capture microdissection of gametes
and zygotes
Chapter 30 Cloning and expression strategies for the
postgenomic analysis of brown algae
Chapter 31 Polyethylene glycol-mediated transformation in
the green macroalga Ulva mutabilis (Chlorophyta):
A forward genetics approach
Index
