Description
Book SynopsisThis detailed book highlights the diverse techniques and applications of proteomics in an accessible, informative, and concise manner.
Table of Contents1. Review of the Real and Sometimes Hidden Costs in Proteomics Experimental Workflows
Aicha Asma Houfani and Leonard James Foster
2. High-Throughput Mass Spectrometry-Based Proteomics with dia-PASEF
Patricia Skowronek and Florian Meier
3. Isolation of Detergent Insoluble Proteins from Mouse Brain Tissue for Quantitative Analysis Using Data Independent Acquisition (DIA)
Cristen Molzahn, Lorenz Nierves, Philipp F. Lange, and Thibault Mayor
4. Rodent Lung Tissue Sample Preparation and Processing for Shotgun Proteomics
Hadeesha Piyadasa, Ying Lao, Oleg Krokhin, and Neeloffer Mookherjee
5. Protein Purification and Digestion Methods for Bacterial Proteomic Analyses
Nicole Hansmeier, Samrachana Sharma, and Tzu-Chiao Chao
6. Mapping Cell Surface Proteolysis with Plasma Membrane-Targeted Subtiligase
Aspasia A. Amiridis and Amy M. Weeks
7. N-Terminomics/TAILS of Tissue and Liquid Biopsies
Anthonia Anowai, Sameeksha Chopra, Barbara Mainoli, Daniel Young, and Antoine Dufour
8. HUNTER: Sensitive Automated Characterization of Proteolytic Systems by N Termini Enrichment from Microscale Specimen
Anuli C. Uzozie, Janice Tsui, and Philipp F. Lange
9. Phosphoproteomics and Organelle Proteomics in Pancreatic Islets
Özum Sehnaz Caliskan, Giorgia Massacci, Natalie Krahmer, and Francesca Sacco
10. Phosphoproteomic Sample Preparation for Global Phosphorylation Profiling of a Fungal Pathogen
Brianna Ball, Jonathan Krieger, and Jennifer Geddes-McAlister
11. Glycopeptide-Centric Approaches for the Characterization of Microbial Glycoproteomes
Nichollas E. Scott
12. Integrated Network Discovery Using Multi-Proteomic Data
Rafe Helwer and Vincent C. Chen
13. Targeted Cross-Linking Mass Spectrometry on Single-Step Affinity Purified Molecular Complexes in the Yeast Saccharomyces cerevisiae
Christian Trahan and Marlene Oeffinger
14. A Crosslinking – Mass Spectrometry Protocol for the Structural Analysis of Microtubule-Associated Proteins
Atefeh Rafiei and David C. Schriemer
15. Comprehensive Interactome Mapping of Nuclear Receptors Using Proximity Biotinylation
Lynda Agbo, Sophie Anne Blanchet, Pata-Eting Kougnassoukou Tchara, Amélie Fradet-Turcotte, and Jean-Philippe Lambert
16. Mining Proteomics Datasets to Uncover Functional Pseudogenes
Anna Meller and François-Michel Boisvert
17. Proteomic Profiling of the Interplay between a Bacterial Pathogen and Host Uncovers Novel Anti-Virulence Strategies
Arjun Sukumaran and Jennifer Geddes-McAlister
18. Affinity-Enrichment of Salmonella-Modified Membranes from Murine Macrophages for Proteomic Analyses
Tzu-Chiao Chao, Samina Thapa, and Nicole Hansmeier
19. Proteomic Profiling of Interplay between Agrobacterium tumefaciens and Nicotiana benthamiana for Improved Molecular Pharming Outcomes
Nicholas Prudhomme, Jonathan R. Krieger, Michael D. McLean, Doug Cossar, and Jennifer Geddes-McAlister
20. Label-Free Quantitative Proteomic Profiling of Fusarium Head Blight in Wheat
Boyan Liu, Danisha Johal, Mitra Serajazari, and Jennifer Geddes-McAlister
21. DIA Proteomics and Machine Learning for the Fast Identification of Bacterial Species in Biological Samples
Florence Roux-Dalvai, Mickaël Leclercq, Clarisse Gotti, and Arnaud Droit
22. Novel Bioinformatics Strategies Driving Dynamic Metaproteomic Studies
Caitlin M.A. Simopoulos, Daniel Figeys, and Mathieu Lavallée-Adam
23. MaxQuant Module for the Identification of Genomic Variants Propagated into Peptides
Pavel Sinitcyn, Maximilian Gerwien, and Jürgen Cox
24. Untargeted Metabolomic Profiling of Fungal Species Populations
Thomas E. Witte and David P. Overy
