Description
Table of ContentsPART I OVERVIEWS
1 Structural basis of protein-protein interactions
Robert Liddington
2 Quantitative analysis of protein-protein interactions
Ziad Eletr and Keith Wilkinson
3 Protein-protein interaction databases
Damian Szklarczyk and Lars Juhl Jensen
4 Computational prediction of protein-protein interactions
Tobias Ehrenberger, Lewis C. Cantley, and Michael B. Yaffe
5 Structure-based computational approaches for small-molecule modulation of protein-protein interactions
David Xu, Bo Wang, and Samy O. Meroueh
6 Targeting protein-protein interactions for drug discovery
David C. Fry
PART II LABEL-FREE BIOSENSORS AND TECHNIQUES
7 Studying protein-protein interactions using surface plasmon resonance
Zenata Nikolovska-Coleska
8 Resonant waveguide grating for monitoring bimolecular interactions
Meng Wu and Min Li
9 Quartz microbalance technology for probing biomolecular interactions
Gabriella T. Heller, Alison R. Mercer-Smith, and Malkiat S. Johal
10 Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry
Sriram Kumaraswamy and Renee Tobias
11 Characterization of protein-protein interactions by isothermal titration calorimetry
Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freiere
12 Sedimentation equilibrium studies
Ian A. Taylor, John F. Eccleston, and Katrin Rittinger
13 Detecting protein-protein interactions by gel filtration chromatography
Yan Bai
14 Using light scattering to determine the stoichiometry of protein complexes
Jeremy Mogridge
15 Circular dichroism (CD) analyses of protein-protein interactions
Norma Greenfield
16 Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy
Peter M. Thompson, Moriah R. Beck, and Sharon L. Campbell
17 Quantitative protein analysis by mass spectrometry
Vishwajeeth Pagala, Anthony High, Xusheng Wang, Haiyan Tan, Kiran Kodali, Ashutosh Mishra, Kanisha Kavdia, Yanji Xu, Zhiping Wu, and Junmin Peng
18 Using peptide arrays created by the SPOT method for defining protein-protein interactions
Yun Young Yim, Katherine Betke, and Heidi Hamm
PART III TAG/AFFINITY-BASED METHODS
19 Fluorescence polarization assay to quantify protein-protein interactions
Ronald T. Raines
20 Förster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions
Alexa L. Mattheyses and Adam I. Marcus
21 Utilizing ELISA to monitor protein-protein interactions
Zusen Weng and Qinjian Zhao
22 Glutathione-S-transferase (GST)-fusion based assays for studying protein-protein interactions
Haris G. Vikis and Kun-Liang Guan
23 Hexahistidine (6xHis) fusion-based assays for protein-protein interactions
Mary C. Puckett
24 Studying protein-protein interactions via blot overlay/Far Western blot
Randy A. Hall
25 Co-immunoprecipitation from transfected cells
Yoshinori Takahashi
26 In vivo protein cross-linking
Fabrice Agou and Michel Véron
PART IV CELL-BASED BIMOLECULAR INTERACTION REPORTER ASSAYS
27 Identification of protein-protein interactions by standard Gal4p-based yeast two-hybrid screening
Jeroen Wagemans and Rob Lavigne
28 Reverse two-hybrid techniques in the yeast Saccharomyces cerevisiae
Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn
29 MAPPIT, a mammalian two-hybrid method for in-cell detection of protein-protein interactions
Irma Lemmens, Sam Lievens, and Jan Tavernier
30 Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells
Nicole E. Brown, Joe B. Blumer, and John R. Hepler
31 Mapping biochemical networks with protein fragment complementation assays
Ingrid Remy and Stephen W. Michnick
32 Detection of protein-protein interaction using bimolecular fluorescence complementation assay
Cau D. Pham
33 Split-luciferase complementation assay to detect channel-protein interactions in live cells
Alexander S. Shavkunov, Syed R. Ali, Neli I. Panova-Elektronova, and Fernanda Laezza
34 Confocal microscopy for intracellular co-localization of proteins
Toshiyuki Miyashita
PART V HIGH THROUGHPUT SCREENING ASSAYS FOR PROTEIN- PROTEIN INTERACTIONS - CASE STUDIES
35 Fluorescence polarization assay to quantify protein-protein interactions in an HTS format
Yuhong Du36 Estrogen receptor alpha/co-activator interaction assay - TR-FRET
Terry W. Moore, Jillian R. Gunther, and John A. Katzenellenbogen
37 High content screening biosensor assay to identify disruptors of p53-hDM2 protein-protein interactions
Yun Hua, Christopher J. Strock. and Paul A. Johnston
38 Discovery of inhibitors of the MDM2-p53 protein-protein interaction
Liu Liu, Denzil Bernard, and Shaomeng Wang
39 Biophysical methods for identifying fragment-based inhibitors of protein-protein interactions
Samuel J. Pfaff, Michael S. Chimenti, Mark J. S. Kelly, and Michelle R. Arkin
