Description
Book SynopsisProtein phosphorylation analysis is a central theme in current analytical biochemistry, cell biology and systems biology. Due to its versatility, specificity and sensitivity, mass spectrometry has developed into a key technology in this field. A set of minor and major instrumental innovations mean that mass spectrometers now exhibit a level of performance, a stability of operation, a relative ease of use, and productivity, which would once have been hard to imagine. This book guides the reader through this prolific field by presenting a collection of personal views and selected examples which cover all the important principles with a focus on electrospray ionization mass spectrometry. It covers: phosphorylation analysis at the peptide, protein and proteome level; manual and automated data evaluation; phosphopeptide enrichment; quantitative aspects; element mass spectrometry; individual analytical strategies, and hints to useful internet resources. This book provides students, graduate students, post-Docs and senior scientists from related areas with a better understanding on molecular protein phosphorylation analysis. Its highest aim is to strengthen the reader''s ability to develop a personal, well-founded opinion on original manuscripts published in this field.
Trade ReviewThe book is a great and truly insightful guide to understand basics of mass spectrometry and its analytical power for protein phosphorylation analysis. * Mogjib Salek *
Overall, this book positively stands out from the crowd of publications related to biochemical applications of MS.The book clearly excels when concepts, strategies, and applications are discussed,The book can be fully recommended to any practitioner in the field of protein analysisa valuable package for every proteomics laboratory.Overall, the book is a welcome addition to the field of mass-spectrometric protein analysis.it was carefully prepared by the publisher, and yet, it is compact and handy. * Jurgen H Gross *
Table of ContentsIntroduction; Biological Basis of Reversible Protein Phosphorylation; Collision Induced Dissociation; MALDI-MS and MS/MS of Phosphopeptides; ESI-MS and MS/MS of Phosphopeptides; Enrichment Technologies for Phosphopeptides/Phosphoproteins; Non-MS Tools for Phosphorylation Analysis; Analytical Strategies for Protein Phosphorylation Analysis; Quantitative Aspects; Internet Tools Related to Phosphorylation Analysis; Summary of Reviews on Protein Phosphorylation Analysis by MS; Outlook
