Description
Book SynopsisProtein Methods Daniel M. Bollag/ Michael D. Rozycki /Stuart J. Edelstein Reviews from the first edition ". it is very well written. would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time.
Table of ContentsPreface xiii
Preparation for Protein Isolation 1
Protein Extraction and Solubilization 27
Protein Concentration Determination 57
Concentrating Protein Solutions 83
Gel Electrophoresis Under Denaturing Conditions 107
Gel Electrophoresis Under Nondenaturing Conditions 155
Isoelectric Focusing and Two-Dimensional Gel Electrophoresis 173
Immunoblotting 195
Ion Exchange Chromatography 231
Gel Filtration Chromatography 271
Affinity Chromatography 301
Hanging Drop Crystallization 353
Appendix 1: Molecular weights of commonly used Chemicals 389
Appendix 2: Molecular weights and Isoelectric Points of Selected Protiens 393
Appendix 3: Ammonium Sulfate Precipitation Table 394
Appendix 4: Spectrophotometer Linearity 397
Appendix 5: Suppliers and Addressed 399
Index 405
