Description

Book Synopsis
Protein Methods Daniel M. Bollag/ Michael D. Rozycki /Stuart J. Edelstein Reviews from the first edition ". it is very well written. would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time.

Table of Contents

Preface xiii

Preparation for Protein Isolation 1

Protein Extraction and Solubilization 27

Protein Concentration Determination 57

Concentrating Protein Solutions 83

Gel Electrophoresis Under Denaturing Conditions 107

Gel Electrophoresis Under Nondenaturing Conditions 155

Isoelectric Focusing and Two-Dimensional Gel Electrophoresis 173

Immunoblotting 195

Ion Exchange Chromatography 231

Gel Filtration Chromatography 271

Affinity Chromatography 301

Hanging Drop Crystallization 353

Appendix 1: Molecular weights of commonly used Chemicals 389

Appendix 2: Molecular weights and Isoelectric Points of Selected Protiens 393

Appendix 3: Ammonium Sulfate Precipitation Table 394

Appendix 4: Spectrophotometer Linearity 397

Appendix 5: Suppliers and Addressed 399

Index 405

Protein Methods

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    A Paperback / softback by Daniel M. Bollag, Michael D. Rozycki, Stuart J. Edelstein

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      Book details

      Publisher John Wiley & Sons Inc
      Published 1 August 1996
      ISBN-13 9780471118374
      978-0471118374
      ISBN-10 0471118370
      Also in:
      Chemistry Scientific equipment, experiments and techniques

      Description

      Book Synopsis
      Protein Methods Daniel M. Bollag/ Michael D. Rozycki /Stuart J. Edelstein Reviews from the first edition ". it is very well written. would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time.

      Table of Contents

      Preface xiii

      Preparation for Protein Isolation 1

      Protein Extraction and Solubilization 27

      Protein Concentration Determination 57

      Concentrating Protein Solutions 83

      Gel Electrophoresis Under Denaturing Conditions 107

      Gel Electrophoresis Under Nondenaturing Conditions 155

      Isoelectric Focusing and Two-Dimensional Gel Electrophoresis 173

      Immunoblotting 195

      Ion Exchange Chromatography 231

      Gel Filtration Chromatography 271

      Affinity Chromatography 301

      Hanging Drop Crystallization 353

      Appendix 1: Molecular weights of commonly used Chemicals 389

      Appendix 2: Molecular weights and Isoelectric Points of Selected Protiens 393

      Appendix 3: Ammonium Sulfate Precipitation Table 394

      Appendix 4: Spectrophotometer Linearity 397

      Appendix 5: Suppliers and Addressed 399

      Index 405

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