Factors inherent to the protein sequence responsible for aggregation and
Table of Contents
Contributors vii
Preface xi
Introduction to the Wiley Series in Protein and Peptide Science xiii
1 Fundamentals of Protein Folding 1
Vladimir N. Uversky
2 Recruiting Unfolding Chaperones to Solubilize Misfolded Recombinant Proteins 63
Rayees U.H. Mattoo and Pierre Goloubinoff
3 Osmolytes as Chemical Chaperones to Use in Protein Biotechnology 77
Ario de Marco
4 Inclusion Bodies in the Study of Amyloid Aggregation 93
Anna Villar-Piqué and Salvador Ventura
5 Protein Aggregation in Unicellular Eukaryotes 117
Marina Caldara, Joris Winderickx, and Vanessa Franssens
6 Structural Properties of Bacterial Inclusion Bodies 151
Antonino Natalello, Diletta Ami, and Silvia Maria Doglia
7 Residue-Specific Structural Studies of Inclusion Bodies 181
Christian Wasmer, Marielle Wälti, Yongli Chen, and Lei Wang
8 Biomedical Applications of Bacterial Inclusion Bodies 203
Imma Ratera, Spela Peternel, Joaquin Seras-Franzoso, Olivia Cano-Garrido, Elena García-Fruitós, Rafael Cubarsí, Esther Vazquez, José Luis Corchero, Escarlata Rodríguez-Carmona, Jaume Veciana, and Antonio Villaverde
9 Aggregation of Recombinant Proteins: Understanding Basic Issues to Overcome Production Bottlenecks 221
Marina Lotti and Loredano Pollegioni
10 Fusion to a Pull-Down Module: Designing Enzymes to Form Biocatalytically Active Insoluble Aggregates 247
Bernd Nidetzky
Index 263
