Description
Book SynopsisThe book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.
Table of ContentsPreface…Table of Contents…Contributing Authors…
Part I Detection, Quantification, and Isolation of Acetylated Protein Targets
1. High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein TargetsBirgit Schilling, Jesse G. Meyer, Lei Wei, Melanie Ott, and Eric Verdin
2. Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass SpectrometryPaula A. Agudelo Garcia, Miranda Gardner, Michael A. Freitas, and Mark R. Parthun
3. Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it ChemistyPavlo Lazarchuk, Sunetra Roy, Katharina Schlacher, and Julia Sidorova
Part II Lysine Acetylation
4. Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLCCatherine W. Njeri, Onyekachi E. Ononye, and Lata Balakrishnan
5. Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In VivoAnahita Lashgari, Jean-Philippe Lambert, and Jacques Côté
6. Site-Specific Lysine Acetylation Stoichiometry across Subcellular CompartmentsAnastasia J. Lindahl, Alexis J. Lawton, Josue Baeza, James A. Dowell, and John M. Denu
7. Lysine Acetylation of Proteins and its Characterization in Human SystemsDavid K. Orren and Amrita Machwe
Part III Sirtuin Targets of Acetylation
8. Molecular and Cellular Characterization of SIRT1 Allosteric ActivatorsMichael B. Schultz, Conrad Rinaldi, Yuancheng Lu, João A. Amorim, and David A. Sinclair
9. Assessment of SIRT2 Inhibitors in Mouse Models of CancerYashira L. Negrón Abril, Irma Fernández, and Robert S. Weiss
Part IV Modulation of DNA Repair by Acetylation
10. Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair Amelia J. Hodges, Steven A. Roberts, and John J. Wyrick
11. Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair EnzymesShrabasti Roychoudhury, Suravi Pramanik, Hannah L. Harris, and Kishor K. Bhakat
12. Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine AcetylationOnyekachi E. Ononye, Catherine W. Njeri, and Lata Balakrishnan
13. Examining the Role of HDACs in DNA Double-Strand Break Repair in NeuronsPing-Chieh Pao, Jay Penney, and Li-Huei Tsai
Part V DNA Interactive Proteins and Processing Enzymes Affected by Acetylation
14. Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop StabilityChenlin Song and Ingrid Grummt
15. Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling Marina Farkas and Steven B. McMahon
Part VI Influence of Acetylation Processes on Neuroepigenetics and Tumorigenesis
16. Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive NeuroepigeneticsCraig Myrum and Peter R. Rapp
17. Functional Analysis of HDACs in TumorigeneisMelissa Hadley, Satish Noonepalle, Debarati Banik, and Alejandro Villagra
