Description
Book SynopsisThe purpose of the book is to introduce platelets, and their functional role in thrombotic and cardiovascular disease, justifying the relevance of platelet proteomics research. Focus then shifts to the recent developments on mass spectrometry (MS)-based proteomics. This chapter shows potential applications for platelet proteomics not yet carried out. It includes examples of post-translational modifications (PTMs) analysis in platelets.
The second part of the book focuses on the main research done so far on platelet proteomics. This includes general proteome mapping by non-gel based separation methods (MudPit), analysis of the general platelet proteome and signaling cascades by gel-based separation methods (2-DE), sub-proteome analyses (secretome/releasate, membrane proteins, organelles). Finally, the last section links the platelet transcriptome and application to disease. This section is highly relevant and includes chapters on proteomics, transcriptomics, functional genomics, sys
Trade Review
"Experienced researchers looking for an up-to-date and comprehensive review of this rapidly developing field will find this book a valuable resource.” (American Society for Mass Spectrometry, 13 December 2011)
Table of ContentsForeword xiii
Preface xv
Acronyms xvii
Part I General Overview: Platelets, Sample Preparation, and Mass Spectrometry-Based Proteomics
1 Platelets and Their Role in Thrombotic and Cardiovascular Disease: The Impact of Proteomic Analysis 3
Ronald G. Stanley, Katherine L. Tucker, Natasha E. Barrett, and Jonathan M. Gibbins
2 Mass-Spectrometry-Based Proteomics: General Overview and Posttranslational Modification Analysis in the Context of Platelet Research 27
Julia Maria Burkhart and Albert Sickmann
3 Sample Preparation Variables in Platelet Proteomics for Biomarker Research 67
Maria Zellner and Rudolf Oehler
Part II Analysis of The Platelet Proteome: Global Approaches and Subproteomes
4 Two-Dimensional Gel Electrophoresis: Basic Principles and Application to Platelet Signaling Studies 89
Ángel García
5 The Platelet Membrane Proteome 111
Yotis A. Senis
6 Proteomics of Platelet Granules, Organelles, and Releasate 139
James P. McRedmond
7 The Platelet Microparticle Proteome 159
David M. Smalley
8 N-Terminal Combined Fractional Diagonal Chromatographic (COFRADIC) Analysis of the Human Platelet Proteome 185
Francis Impens, Kenny Helsens, Niklaas Colaert, Lennart Martens, Joël Vandekerckhove, and Kris Gevaert
Part III Integrated “Omics” and Application to Disease
9 Serial Analysis of Gene Expression (SAGE) For Studying the Platelet and Megakaryocyte Transcriptome 209
Michael G. Tomlinson
10 The Application of Microarray Analysis and Its Integration with Proteomics for Study of Platelet-Associated Disorders 231
Dmitri V. Gnatenko and Wadie F. Bahou
11 Platelet Functional Genomics 253
Isabelle I. Salles, Marie N. O’Connor, Daphne C. Thijssen-Timmer, Katleen Broos, and Hans Deckmyn
12 Systems Biology to Study Platelet-Related Bleeding Disorders 285
Jan-Willem N. Akkerman and Bernard De Bono
13 Platelet Proteomics in Transfusion Medicine 321
Thomas Thiele, Leif Steil, Uwe Völker, and Andreas Greinacher
14 Cardiovascular Proteomics 341
Fernando Vivanco, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, and Gloria Álvarez-Llamas
Index 381
