Description
Book SynopsisThis volume provides cutting edge protocols for using optogenetic and opto-chemical techniques across a wide spectrum of applications. Chapters guide readers through methods in neuro-optogenetics, including methods to dissect local and long-range connectivity ex vivo, investigate G-protein coupled receptors, study astrocyte-neuron networks in vitro and in vivo, employ upconversion nanoparticles for transcranial optogenetic stimulation, and use Adeno-associated virus (AAV) for delivery of optogenetic constructs. At the same time, this book covers a range of methods for non-neuronal optogenetics, including various applications of light-oxygen-voltage domains or cryptochromes for control of nucleocytoplasmic transport, transgene expression and chromatin recruitment. The book further presents protocols for traceless light-control of gene expression with melanopsin, light-inducible cAMP production, use of light-regulated receptor tyrosine kinases (Opto-RTKs), synthesis of light-dependent bi
Table of Contents1. Studying Neuronal Function Ex Vivo using Optogenetic Stimulation and Patch Clamp
Ayla Aksoy-Aksel, Julien Genty, Martin Zeller, and Ingrid Ehrlich
2. Optogenetic Techniques for Probing G Protein-Coupled Receptor Signaling
Nohely Abreu and Joshua Levitz
3. Melanopsin for Time-Controlling Activation of Astrocyte-Neuron Networks
Sara Mederos, Candela González-Arias, and Gertrudis Perea
4. Near-infrared Deep Brain Stimulation in Living Mice
Shuo Chen
5. Lab-scale Production of Recombinant Adeno-associated Viruses (AAV) for Expression of Optogenetic Elements
Janina Haar, Chiara Krämer, and Dirk Grimm
6. AAV-mediated Gene Delivery to Foveal Cones
Stéphane Bertin, Elena Brazhnikova, Céline Jaillard, José-Alain Sahel, and Deniz Dalkara
7. Engineering Optogenetic Protein Analogs
Bei Liu, Daniel J. Marston, and Klaus M. Hahn
8. Optogenetic Control of Nucleocytoplasmic Protein Transport
Daniel Weis and Barbara Di Ventura
9. CRISPR Labeling Light-inducible
Mareike D. Hoffmann, Felix Bubeck, and Dominik Niopek
10. Optogenetic Control of Gene Expression using Cryptochrome 2 and a Light-activated Degron
Carmen N. Hernández-Candia and Chandra L. Tucker
11. Optogenetic Downregulation of Protein Levels to Control Programmed Cell Death in Mammalian Cells with a Dual Blue-Light Switch
Patrick Fischbach, Patrick Gonschorek, Julia Baaske, Jamie A. Davies, Wilfried Weber, and Matias D. Zurbriggen
12. Tracing Reversible Light-induced Chromatin Binding with Near-infrared Fluorescent Proteins
Anne Rademacher, Fabian Erdel, Jorge Trojanowski, and Karsten Rippe
13. Construction of a Multiwell Light-induction Platform for Traceless Control of Gene Expression in Mammalian Cells
Maysam Mansouri, Samson Lichtenstein, Tobias Strittmatter, Peter Buchmann, and Martin Fussenegger
14. Dual-activation of cAMP Production through Photo-Stimulation or Chemical Stimulation
Nyla Naim, Jeff M. Reece, Xuefeng Zhang, and Daniel L. Altschuler
15. Synthesis of a Light-controlled Phytochrome-based Extracellular Matrix with Reversibly Adjustable Mechanical Properties
Maximilian Hörner, Philipp Hoess, Ramona Emig, Balder Rebmann, and Wilfried Weber
16. Design and Application of Light-regulated Receptor Tyrosine Kinases
Stephanie Kainrath and Harald Janovjak
17. All-optical Miniaturized Co-culture Assay of Voltage-gated Ca2+ Channels
Viviana Agus and Harald Janovjak
18. Optogenetics and CRISPR: A New Relationship Built to Last
Jan Mathony, Mareike Daniela Hoffmann, and Dominik Niopek
