Description
Book SynopsisPeroxynitrite is a powerful oxidiser which can damage a wide array of molecules within cells, including DNA and proteins, leading to apoptosis, inflammation or cancer. Peroxynitrite detection and quantification provides critical information in understanding its biological implications. Attempts to investigate the behavior of peroxynitrite in vivo and in vitro have been hampered by the difficulty in detecting this highly reactive oxygen species. This book presents the current state of the art in this research field with contributions from scientific leaders in the field. The chapters make clear the associated challenges and development for selective and sensitive detection of peroxynitrite. This book is a timely addition to the literature, as the first in the field, dedicated to detecting this molecule in vivo. It will be welcomed by the community particularly medicinal and analytical chemists, developers of sensors and probes and analytical equipment manufacturers.
Table of ContentsPeroxynitrite, the basics; Quantifying peroxynitrite: bridging the gap between chemistry, biology and immunology; Methods of peroxynitrite synthesis in the context of the development and validation of peroxynitrite sensors; Peroxynitrite sensitive electrochemically active matrices; Electrochemical detection of peroxynitrite in biological solutions: challenges and perspectives; Real time monitoring of peroxynitrite by stimulation of macrophages with microelectrodes; Electrophoretic methods for separation of peroxynitrite and related compounds; Investigation of peroxynitrite-biomembrane interactions using biomimetic interfaces; Recent approaches to enhance selectivity of peroxynitrite detection; Development of fluorescent probes for the detection of peroxynitrite; Reversible near infrared probes for peroxynitrite monitoring;
