Description
Book SynopsisThe formation of disulfide bonds is probably the most influential modification of peptides and proteins. An elaborate set of cellular machinery exists to catalyze and guide this process. In recent years, significant developments have been made in both our understanding of the in vivo situation and the in vitro manipulation of disulfide bonds. This is the first monograph to provide a comprehensive overview of this exciting and rapidly developing area. It offers in-depth insights into the mechanisms of in vivo and in vitro oxidative folding of proteins as well as mono- and multiple-stranded peptides. Procedures applied for laboratory and industrial purposes are also discussed by top experts in the field. The book describes the enzymes involved in the correct oxidative folding of cysteine-containing proteins in prokaryotes and eukaryotes. It then goes on to discuss the mimicking of these enzymes for successful in vitro folding of proteins (including synthetic replicates) and to deal with
Table of ContentsForeword; Preface; Oxidative Folding of Proteins in vivo; Disulfide-bond Formation and Isomerization in Prokaryotes; The Periplasm of E. coli - Oxidative Folding of Recombinant Proteins; Oxidative Protein Folding in Mitochondria; Oxidative Folding in the Endoplasmic Reticulum; The Ero1 Sulfhydryl Oxidase and the Oxidizing Potential of the Endoplasmic Reticulum; Eukaryotic Protein Disulfide-isomerases and their Potential in the Production of Disulfide-bonded Protein Products: What We Need to Know but Do Not!; Cellular Responses to Oxidative Stress; Oxidative Folding of Proteins in vitro; Strategies for the Oxidative in vitro Refolding of Disulfide-bridge-containing Proteins; Redox Potentials of Cysteine Residues in Peptides and Proteins: Methods for their Determination; Engineered Disulfide Bonds for Protein Design; Selenocysteine as a Probe of Oxidative Protein Folding; Oxidative Folding of Peptides in vitro; Regioselective Disulfide Formation; Folding Motifs of Cystine-rich Peptides; Double-stranded Cystine Peptides; Multiple-strand Cystine Peptides; Cystine-based Scaffolds for Functional Miniature Proteins; Selenocystine Peptides – Synthesis, Folding and Applications; Subject Index
