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Book SynopsisTable of Contents1. Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow
Naig Gueguen, Guy Lenaers, Pascal Reynier, Volkmar Weissig, and Marvin Edeas
2. Preparation of ‘Functional’ Mitochondria – A Challenging Business
Stefan Lehr, Sonja Hartwig, and Jorg Kotzka
3. Isolation and Quality Control of Functional Mitochondria
Sonja Hartwig, Jorg Kotzka, and Stefan Lehr
4. Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient
Jacob L. Léger, Nicolas Pichaud, and Luc H. Boudreau
5. Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues
Alessandro Gaviraghi, Yan Aveiro, Stephanie S. Carvalho, Rodiesley S. Rosa, Matheus P. Oliveira, and Marcus F. Oliveira
6. Analysis of Mitochondrial Retrograde Signaling In Yeast Model Systems
Nicoletta Guaragnella, Maša Ždralević, Zdena Palková, and Sergio Giannattasio
7. Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes
Gisela Beutner and George A. Porter, Jr.
8. Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in Drosophila Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging
Lori M. Buhlman, Petros P. Keoseyan, Kathryn Houlihan, and Amber N. Juba
9. Assessment of Mitochondrial Cell Metabolism By Respiratory Chain Electron Flow Assays
Flavia Radogna, Déborah Gérard, Mario Dicato, and Marc Diederich
10. Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues
Juan C. Landoni, Liya Wang, and Anu Suomalainen
11. Single Particle-Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses Of Individual Mitochondria
Camille Colin, Emmanuel Suraniti, Emma Abell, Audrey Sémont, Neso Sojic, Philippe Diolez, and Stéphane Arbault
12. Investigation of Mitochondrial ADP-ribosylation via Immunofluorescence
Ann-Katrin Hopp and Michael O. Hottiger
13. Assessment of Mitochondrial Ca2+ Uptake
András T. Deak, Claire Jean-Quartier, Alexander I. Bondarenko, Lukas N. Groschner, Roland Malli, Wolfgang F. Graier, and Markus Waldeck-Weiermair
14. Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices
Andrey Y. Vinokurov, Viktor V. Dremin, Gennadii A. Piavchenko, Olga A. Stelmashchuk, Plamena R. Angelova, and Andrey Y. Abramov
15. Evaluation of Mitochondria Content and Function in Live Cells by Multi-color Flow Cytometric Analysis
Hsiu-Han Fan, Tsung-Lin Tsai, Ivan L. Dzhagalov, and Chia-Lin Hsu
16. Analysis of Mitochondrial Dysfunction during Cell Death
Vladimir Gogvadze and Boris Zhivotovsky
17. Modified Blue-Native Gel Approach for Analysis of Respiratory Super-Complexes
Sergiy M. Nadtochiy, Megan Ngai, and Paul S. Brookes
18. Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane
Piotr Bednarczyk, Rafał P. Kampa, Shur Kucman, Aleksandra Sęk, Agnieszka Walewska, and Piotr Koprowski
19. Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway
Ana S. Almeida, Cláudia Figueiredo-Pereira, and Helena L.A. Vieira
20. 3D Optical Cryo-Imaging Method: A Novel Approach To Quantify Renal Mitochondrial Bioenergetics Dysfunction
Shima Mehrvar, Amadou K. S. Camara, and Mahsa Ranji
21. Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology
Liping Yu, Brian D. Fink, and William I. Sivitz
22. High-Throughput Image Analysis of Lipid-Droplet-Bound Mitochondria
Nathanael Miller, Dane Wolf, Nour Alsabeeh, Kiana Mahdaviani, Mayuko Segawa, Marc Liesa, and Orian Shirihai
23. Cell Energy Budget Platform for Multi-Parametric Assessment of Cell and Tissue Metabolism
Dmitri B. Papkovsky and Alexander V. Zhdanov
24. Fluorescence-based Assay for Measuring OMA1 Activity
Julia Tobacyk and Lee Ann MacMillan-Crow
25. Studying Mitochondrial Network Formation by in vivo and in vitro Reconstitution Assay
Wanqing Du, Xiangjun Di, and Qian Peter Su
26. Extraction of Functional Mitochondria Based on the Membrane Stiffness
Md Habibur Rahman, Qinru Xiao, Shirui Zhao, An-Chi Wei, and Yi-Ping Ho
27. A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing
Amanda L. Souza and Gary J. Patti
28. A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled With Mass Spectrometry
Matej Kohutiar and Adam Eckhardt
29. In vivo Visualization And Quantification of Mitochondrial Morphology in C. elegans
R. de Boer, R. L. Smith, W. H. De Vos, E. M. M. Manders, andH. van der Spek
30. Assessing Impact of Platinum Complexes on Mitochondrial Functions
Suxing Jin and Xiaoyong Wang
31. In-silico Modeling of the Mitochondrial Pumping Complexes with Markov State Models
Roger Springett
32. Monitoring the Mitochondrial Presequence Import Pathway in Living Mammalian Cells with a New Molecular Biosensor
Maxime Jacoupy, Emeline Hamon-Keromen, and Olga Corti
