Description
Book SynopsisThe recent explosion in the use of analytical chemistry, particularly in the biological sciences, has led to a need for fast, reliable and highly sensitive tools able to handle small sample sizes. This book illustrates how microfluidics and lab-on-a-chip devices can satisfy the growing need for miniaturized and enhanced analysis. They lend themselves well to mass spectrometric detection as they use samples in the low microlitre range and are handled on a chip. Miniaturization and Mass Spectrometry focuses on one particular technique, mass spectrometry, whose popularity has increased dramatically in the last two decades with the increase in use of biological analysis and the development of two soft ionization techniques, ESI and MALDI. These enable the analysis of large but fragile biological molecules such as DNA, proteins and oligosaccharides. The book starts with an introduction to the coupling of microfluidics to mass spectrometry techniques. It then goes on demonstrate the advantag
Table of ContentsChapter 1. Introduction; Chapter 2. The coupling of microfabricated fluidic devices with electrospray ionization mass spectrometers; Chapter 3. A silicon-based microfabricated nozzle with integrated counterelectrode and its applications combined with mass spectrometry; Chapter 4. PDMS ESI emitter tips; Chapter 5. Microfabricated nanoelectrospray emitter tips based on a microfluidic capillary slot; Chapter 6. Microfabricated parylene electrospray tips Integrated with cyclo-olefin microchips for ESI-MS; Chapter 7. Microfluidic bioanalytical platforms with mass spectrometry detection for biomarker discovery and screening; Chapter 8. Modular microfluidics devices combining multi-dimensional separations; Applications to targeted proteomics analyses of complex cellular extracts; Chapter 9. Simple chip-based interfaces for on-line nanospray mass spectrometry; Chapter 10. On-line and off-line MALDI from a microfluidic device; Chapter 11. Microfluidic platforms enabling on-line monitoring of chemical reactions by mass spectrometry; Chapter 12. MALDI-TOF mass spectrometry and digital microfluidics for the investigation of pre-steady state enzyme kinetics; Chapter 13. Development of miniaturized MALDI time-of-flight mass spectrometers for homeland security and clinical diagnostics.
