Description
Book SynopsisA Step-by-Step Guide to Present and Future Uses of Microarray Technology
Microarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts in both academia and industry, Microarray Methods and Protocols is a turn-by-turn roadmap through the processes necessary to perform a successful microarray experiment.
This easy to use book addresses the fundamental aspects of preparing and processing microarrays and bead arrays, labeling, and detection. It also includes a detailed How it Works section that discusses the underlying principles of a number of techniques. Troubleshooting guides offer additional advice for the successful performance of more than 100 protocols in 10 chapters that cover work i
Trade Review
The book will be useful for researchers in protein chemistry and biochemistry, molecular biologists, and teachers and students of medical schools and universities specializing in microarray methods.
--Doctor of Biological Sciences, G. Ya. Wiederschain.
Biochemsitry - Moscow. 2010, VOL 75, No 9,1182
Table of ContentsIntroduction to Microarray Technologies. Nucleic Acid Sample Preparation. Solid-Phase Substrates for Nucleic Acid Microarrays. Protein Sample Preparation for Microarrays. Solid-Phase Chemistries for Protein Microarrays. Protein Microarrays: The Link between Genomics and Proteomics. Bead Arrays: An Introduction to Multiplexed Bead-Based Assays for Proteins. Carbohydrate Arrays. Lectin Microarrays. Printing Methods. Appendix A: Microarray Reagent, Materials, and Equipment Sources. Appendix B: Image Analysis. Index.
