Description
Book SynopsisThis detailed book explores examples of current
in vitro and
in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer
in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful
Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Table of Contents1. Methods for Forming Giant Unilamellar Fatty Acid Vesicles
Lauren A. Lowe, Daniel W.K. Loo, and Anna Wang
2. Preparing Ion Channel Switch Membrane-Based Biosensors
Amani Alghalayini, Charles G. Cranfield, Bruce A. Cornell, and Stella M. Valenzuela
3. Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid Bilayer
Charles G. Cranfield, Anton Le Brun, Alvaro Garcia, Bruce A. Cornell, and Stephen A. Holt
4. Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes
Tadas Penkauskas, Filipas Ambrulevičius, and Gintaras Valinčius
5. Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes
Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster
6. Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs)
Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster
7. Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes
Lissy M. Hartmann, Alvaro Garcia, Evelyne Deplazes, and Charles G. Cranfield
8. De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer
Kan Shoji
9. Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations
Sheikh I. Hossain, Mohammad Z. Islam, Suvash C. Saha, and Evelyne Deplazes
10. Establishing a Lipid Bilayer for Molecular Dynamics Simulations
Robby Manrique
11. Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins
Amanda Buyan and Ben Corry
12. Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling
Kathleen Wood
13. Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation
Michael H.L. Nguyen, Mitchell DiPasquale, Stuart R. Castillo, and Drew Marquardt
14. Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering
Mitchell DiPasquale, Michael H.L. Nguyen, Stuart R. Castillo, Frederick A. Heberle, and Drew Marquardt
15. Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers
Stephen A. Holt, Tara E. Oliver, and Andrew R.J. Nelson
16. Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs) Kinga Burdach, Damian Dziubak, and Slawomir Sek
17. Manipulation of Lipid Membranes with Thermal Stimuli
Karolina Spustova, Lin Xue, Ruslan Ryskulov, Aldo Jesorka, and Irep Gözen
18. Analyzing Morphological Properties of Early-Stage Toxic Amyloid β Oligomers by Atomic Force Microscopy
Dusan Mrdenovic, Jacek Lipkowski, and Piotr Pieta
19. Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains
Romina F. Vázquez, Erasmo Ovalle-García, Armando Antillón, Iván Ortega-Blake, Carlos Muñoz-Garay, and Sabina M. Maté
20. Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR
Elvis Pandzic, Renee Whan, and Alex Macmillan
21. Bacterial Dye Release Measures in Response to Antimicrobial Peptides
Srikanth Dumpati and Debarun Dutta
22. Quantitative Measurements of Membrane Lipid Order in Yeast and Fungi
Maria Makarova and Dylan M. Owen
