Description
Book SynopsisThis second edition is thoroughly revised, updated and enlarged, for the readers. In this second edition, six new chapters have been added: Bone marrow trephine biopsy; Tru-cut biopsy or Core needle biopsy; Diagnostic use of immunhistochemistry in surgical pathology; Microarrays and DNA sequencing; Microdissection and different types of polymerase chain reaction; and Quality control in histopathology.
The chapter on Frozen Section has been fully revised and more practical information have been included, keeping in mind the problems faced by the users during cryostat/frozen sections. Molecular diagnostic changes at a glance in different diseases, tumors and hereditary diseases have been added in the Appendix in Tabular form (almost like a new chapter!) and hope it will be useful for different examinations as last minute revision.
Most of these new chapters have been added in this second edition at the request of readers, especially postgraduate students who are pursuing an MD in Pathology.
Table of Contents1. Tissue Fixation and Fixatives
2. Tissue Processing, Microtomy and Paraffin Sections
3. Cryostat and Frozen Section
4. Staining of Tissues—Basic Concept
5. Routine Hematoxylin and Eosin Stain
6. Carbohydrates
7. Lipids
8. Proteins and Nucleic Acid Staining
9. Connective Tissue Staining
10. Pigments and Minerals
11. Bone and Decalcification
12. Nervous System
13. Some Special Stains
14. Enzyme Histochemistry and Histochemical Stains
15. Immunohistochemistry
16. Cell Block and Diagnostic Cytopathology
17. Plastic/Resin Embedding Media
18. Electron Microscopy
19. Immunofluorescence Techniques
20. Museum Technique
21. Light Microscopy
22. Molecular Diagnostic Techniques and Its Applications
23. In Situ Hybridization and Fluorescent In Situ Hybridization
24. Bone Marrow Trephine Biopsy
25. Tru-cut Biopsy or Core Needle Biopsy
26. Diagnostic Use of Immunohistochemistry in Surgical Pathology
27. Microarrays and DNA Sequencing
28. Microdissection and Different Types of Polymerase Chain Reaction
29. Quality Control in Histopathology
Appendices
Glossary
Index
